Western blot analysis using metal-nitrilotriacetate conjugated CdSe/ZnS quantum dots

Analytical Biochemistry

Volumn 379, Issue 1, 2008, Pages 124-126

  Kim, Myung Jin ^a   Park, Hye Young ^a   Kim, Junwon ^a   Ryu, Jiyoung ^a   Hong, Sukmin ^a   Han, Sung Jun ^a   Song, Rita ^a  

^a Institute Pasteur Korea   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; CADMIUM COMPOUNDS; ELECTROPHORESIS; II-VI SEMICONDUCTORS; METAL ANALYSIS; NANOCRYSTALS; OPTICAL PROPERTIES;

CDSE/ZNS QUANTUM DOTS; DETECTION ABILITY; PROTEIN DETECTION; WESTERN-BLOT ANALYSIS;

SEMICONDUCTOR QUANTUM DOTS;

CADMIUM SELENIDE; COBALT; NICKEL; NITRILOTRIACETIC ACID; QUANTUM DOT; ZINC SULFIDE;

ARTICLE; CONTROLLED STUDY; PRIORITY JOURNAL; PROTEIN ANALYSIS; SENSITIVITY ANALYSIS; WESTERN BLOTTING;

EID: 46549089870     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2008.04.032     Document Type: Article

References (14)

1. Renart J., Reiser J., and Stark G.R. Transfer of proteins from gels to diazobenzyloxymethyl-paper and detection with antisera: A method for studying antibody specificity and antigen structure. Proc. Natl. Acad. Sci. USA 76 (1979) 3116-3120
2. Towbin H., Staehelin T., and Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc. Natl. Acad. Sci. USA 76 (1979) 4350-4354
3. Porath J., Carlsson J., Olsson I., and Belfrage G. Metal chelate affinity chromatography, a new approach to protein fractionation. Nature 258 (1975) 598-599
4. Alivisatos P. The use of nanocrystals in biological detection. Nat. Biotechnol. 22 (2004) 47-52
5. Michalet X., Pinaud F.F., Bentolila L.A., Tsay J.M., Doose S., Li J.J., Sundaresan G., Wu A.M., Gambhir S.S., and Weiss S. Quantum dots for live cells, in vivo imaging, and diagnostics. Science 307 (2005) 538-544
6. Bakalova R., Zhelev Z., Ohba H., and Baba Y. Quantum dot-based Western blot technology for ultrasensitive detection of tracer proteins. J. Am. Chem. Soc. 127 (2005) 9328-9329
7. Lata S., Reichel A., Brock R., Tampé R., and Piehler J. High-affinity adaptors for switchable recognition of histidine-tagged proteins. J. Am. Chem. Soc. 127 (2005) 10205-10215
8. Guignet E.G., Hovius R., and Vogel H. Reversible site-selective labeling of membrane proteins in live cells. Nat. Biotechnol. 22 (2004) 440-444
9. Tortiglione C., Quarta A., Tino A., Manna L., Cingolani R., and Pellegrino T. Synthesis and biological assay of GSH functionalized fluorescent quantum dots for staining Hydra vulgaris. Bioconj. Chem. 18 (2007) 829-835
10. Wulfing C., Lombardero J., and Pluckthun A. An Escherichia coli protein consisting of a domain homologous to FK506-binding proteins (FKBP) and a new metal binding motif. J. Biol. Chem. 269 (1994) 2895-2901
11. Cho S.G., Kim J.W., Lee Y.H., Hwang H.S., Kim M.S., Ryoo K., Kim M.J., Noh K.T., Kim E.K., Cho J.H., Yoon K.W., Cho E.G., Park H.S., Chi S.W., Lee M.J., Kang S.S., Ichijo H., and Choi E.J. Identification of a novel antiapoptotic protein that antagonizes ASK1 and CAD activities. J. Cell. Biol. 163 (2003) 71-81
12. Jamieson T., Bakhshi R., Petrova D., Pocock R., Imani M., and Seifalian A.M. Biological applications of quantum dots. Biomaterials 28 (2007) 4717-4732
13. Azzazy H.M., Mansour M.M., and Kazmierczak S.C. From diagnostics to therapy: Prospects of quantum dots. Clin. Biochem. 40 (2007) 917-927
14. Makrides S.C., Gasbarro C., and Bello J.M. Bioconjugation of quantum dot luminescent probes for Western blot analysis. BioTechniques 39 (2005) 501-506