Immobilization of the cross-linked para-nitrobenzyl esterase of Bacillus subtilis aggregates onto magnetic beads

Process Biochemistry

Volumn 45, Issue 2, 2010, Pages 259-263

  Park, Jae Min ^a   Kim, Mina ^a   Park, Ju Yong ^b   Lee, Dong Hun ^a   Lee, Kyu Ho ^c   Min, Jiho ^b   Kim, Yang Hoon ^a  

^a Chungbuk National University   (South Korea)

^b Chonbuk National University   (South Korea)

^c Hankuk University of Foreign Studies   (South Korea)

Author keywords

Bacillus subtilis; Cross linked enzyme aggregates; Immobilization; Magnetic beads; Para nitrobenzyl esterase

Indexed keywords

ACTIVITY RECOVERY; AQUEOUS MEDIUM; BACILLUS SUBTILIS; CROSS-LINKED ENZYME AGGREGATES; FREEFORMS; FUNCTIONALIZED; GLUTARALDEHYDES; INITIAL ACTIVITY; MAGNETIC BEADS; MAGNETIC SUPPORT; REACTION TIME; RESIDUAL ENZYMES; TEMPERATURE SETTING;

AGGREGATES; ALDEHYDES; AMINES; BACTERIOLOGY; COUPLING AGENTS; ENZYME ACTIVITY; ENZYMES; MAGNETISM; REUSABILITY;

ENZYME IMMOBILIZATION;

BACILLUS SUBTILIS;

EID: 71249098174     PISSN: 13595113     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.procbio.2009.09.015     Document Type: Article

References (14)

1. Chen Y.R., Usui S., Queener S.W., and Yu C.A. Purification and properties of p-nitrobenzyl esterase from Bacillus subtilis. J Ind Microbiol Biotechnol 15 (1995) 10-18
2. Zock J., Cantwell C., Swartling J., Hodges R., Pohl T., Sutton K., et al. The Bacillus subtilis pnbA gene encoding p-nitrobenzyl esterase: cloning, sequence and high-level expression in Escherichia coli. Gene 151 (1994) 37-43
3. Brannon D.R., Mabe J.A., and Fukuda D.S. De-esterification of cephalosporin para-nitrobenzyl esters by microbial enzymes. J Antibiot 29 (1976) 121-124
4. Moore J.C., and Arnold F.H. Directed evolution of a para-nitrobenzyl esterase for aqueous-organic solvents. Nat Biotechnol 14 (1996) 458-467
5. Giver L., Gershenson A., Freskgard P.O., and Arnold F.H. Directed evolution of a thermostable esterase. Proc Natl Acad Sci USA 95 (1998) 12809-12813
6. Moore J.C., Jin H.M., Kuchner O., and Arnold F.H. Strategies for the in vitro evolution of protein function: enzyme evolution by random recombination of improved sequences. J Mol Biol 272 (1997) 336-347
7. Hanefeld U., Gardossi L., and Magner E. Understanding enzyme immobilisation. Chem Soc Rev 38 (2009) 453-468
8. Schoevaart R., Wolbers M.W., Golubovic M., Ottens M., Kieboom A.P., van Rantwijk F., et al. Preparation, optimization, and structures of cross-linked enzyme aggregates (CLEAs). Biotechnol Bioeng 87 (2004) 754-762
9. Aytar B.S., and Bakir U. Preparation of cross-linked tyrosinase aggregates. Process Biochem 43 (2008) 125-131
10. Wilson L., Illanes A., Soler L., and Henri{dotless}́quez. Effect of the degree of cross-linking on the properties of different CLEAs of penicillin acylase. Process Biochem 44 (2009) 322-326
11. Bílková Z., Slováková M., Horák D., Lenfeld J., and Churácek J. Enzymes immobilized on magnetic carriers: efficient and selective system for protein modification. J Chromatogr B: Anal Technol Biomed Life Sci 770 (2002) 177-181
12. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72 (1976) 248-254
13. Kim Y.H., Lee J.W., and Moon S.H. Uniqueness of microbial cutinases in hydrolysis of p-nitrophenyl esters. J Microbiol Biotechnol 13 (2003) 57-63
14. Broun G.H. Chemically aggregated enzymes. In: Mosbach K. (Ed). Methods in enzymology (1997), Academic Press, New York 263-269