In vitro and in vivo relaxation of corpus cavernosum smooth muscle by the selective myosin ii inhibitor, blebbistatin

Journal of Sexual Medicine

Volumn 6, Issue 10, 2009, Pages 2661-2671

  Zhang, Xin hua ^a,b   Aydin, Memduh ^a   Kuppam, Dwaraka ^a   Melman, Arnold ^a   DiSanto, Michael E ^a  

^a ALBERT EINSTEIN COLLEGE OF MEDICINE   (United States)

^b FIRST AFFILIATED HOSPITAL OF GUANGXI MEDICAL UNIVERSITY   (China)

Author keywords

Cross bridge cycling; Endothelin; Erectile function; Erectogenic agent; Myosin; Smooth muscle contraction

Indexed keywords

BLEBBISTATIN; ENDOTHELIN 1; MYOSIN II; PHENYLEPHRINE; POTASSIUM CHLORIDE; FUSED HETEROCYCLIC RINGS;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; AORTA; ARTICLE; BLADDER; CONTROLLED STUDY; CORPUS CAVERNOSUM; DOSE RESPONSE; DRUG ACTIVITY; DRUG BLOOD LEVEL; DRUG DOSE INCREASE; DRUG DOSE REDUCTION; DRUG MEGADOSE; DRUG PROTEIN BINDING; DRUG STRUCTURE; ERECTILE DYSFUNCTION; IN VITRO STUDY; IN VIVO STUDY; INTRACAVERNOUS PRESSURE; MALE; MALE GENITAL SYSTEM SURGERY; MEAN ARTERIAL PRESSURE; NONHUMAN; PENIS ERECTION; PRIORITY JOURNAL; RAT; SMOOTH MUSCLE RELAXATION; SMOOTH MUSCLE TONE; TISSUE PRESSURE; ADULT; AGED; ANIMAL; ANTAGONISTS AND INHIBITORS; DRUG EFFECTS; HUMAN; MIDDLE AGED; MUSCLE CONTRACTION; MUSCLE RELAXATION; PENIS; SMOOTH MUSCLE; SPRAGUE DAWLEY RAT;

ADULT; AGED; ANIMALS; AORTA; DOSE-RESPONSE RELATIONSHIP, DRUG; HETEROCYCLIC COMPOUNDS WITH 4 OR MORE RINGS; HUMANS; IN VITRO TECHNIQUES; MALE; MIDDLE AGED; MUSCLE CONTRACTION; MUSCLE RELAXATION; MUSCLE, SMOOTH; MYOSIN TYPE II; PENIS; PHENYLEPHRINE; RATS; RATS, SPRAGUE-DAWLEY; URINARY BLADDER;

EID: 70649101495     PISSN: 17436095     EISSN: 17436109     Source Type: Journal    
DOI: 10.1111/j.1743-6109.2009.01424.x     Document Type: Article

References (35)

1. Andersson KE, Wagner G. Physiology of penile erection [review]. Physiol Rev 1995, 75:191-236.
2. DiSanto ME. Contractile mechanisms in diabetes-related erectile dysfunction. Curr Pharm Des 2005, 11:3995-4010.
3. Zhang XH, Filippi S, Morelli A, Vignozzi L, Luconi M, Donati S, Forti G, Maggi M. Testosterone restores diabetes-induced erectile dysfunction and sildenafil responsiveness in two distinct animal models of chemical diabetes. J Sex Med 2006, 3:253-64.
4. Zhang XH, Morelli A, Luconi M, Vignozzi L, Filippi S, Marini M, Vannelli GB, Mancina R, Forti G, Maggi M. Testosterone regulates PDE5 expression and in vivo responsiveness to tadalafil in rat corpus cavernosum. Eur Urol 2005, 47:409-16.
5. Adelstein RS, Eisenberg E. Regulation and kinetics of the actin-myosin-ATP interaction [review]. Annu Rev Biochem 1980, 49:921-56.
6. Gu H, Martin H, Barsotti RJ, Labelle EF. Rapid increase in inositol phosphate levels in norepinephrine-stimulated vascular smooth muscle. Am J Physiol Cell Physiol 1991, 261:C17-22.
7. Morelli A, Filippi S, Vignozzi L, Mancina R, Maggi M. Physiology of erectile function: An update on intracellular molecular processes. EAU-EBU Update Ser 2006, 4:96-108.
8. Wingard C, Fulton D, Husain S. Altered penile vascular reactivity and erection in the Zucker obese-diabetic rat. J Sex Med 2007, 4:348-62.
9. DiSanto ME, Wang Z, Menon C, Zheng Y, Chacko T, Hypolite J, Broderick G, Wein AJ, Chacko S. Expression of myosin isoforms in smooth muscle cells in the corpus cavernosum penis. Am J Physiol 1998, 275:C976-87.
10. Sward K, Mita M, Wilson DP, Deng JT, Susnjar M, Walsh MP. The role of RhoA and Rho-associated kinase in vascular smooth muscle contraction. Curr Hypertens Rep 2003, 5:66-72.
11. Ghalayini IF. Nitric oxide-cyclic GMP pathway with some emphasis on cavernosal contractility. Int J Impot Res 2004, 16:459-69.
12. Babij P, Kelly C, Periasamy M. Characterization of a mammalian smooth muscle myosin heavy-chain gene: Complete nucleotide and protein coding sequence and analysis of the 5′ end of the gene. Proc Natl Acad Sci USA 1991, 88:10676-80.
13. DiSanto ME, Cox RH, Wang Z, Chacko S. NH2-terminal-inserted myosin II heavy chain is expressed in smooth muscle of small muscular arteries. Am J Physiol 1997, 272:C1532-42.
14. Hypolite JA, DiSanto ME, Zheng YM, Chang SH, Wein AJ, Chacko S. Regional variation in myosin isoforms and phosphorylation at the resting tone in urinary bladder smooth muscle. Am J Physiol-Cell Physiol 2001, 280:C254-64.
15. Kelley CA, Takahashi M, Yu JH, Adelstein RS. An insert of seven amino acids confers functional differences between smooth muscle myosins from the intestines and vasculature. J Biol Chem 1993, 268:12848-54.
16. Straight AF, Cheung A, Limouze J, Chen I, Westwood NJ, Sellers JR, Mitchison TJ. Dissecting temporal and spatial control of cytokinesis with a myosin II inhibitor. Science 2003, 299:1743-7.
17. Limouze J, Straight AF, Mitchison T, Sellers JR. Specificity of blebbistatin, an inhibitor of myosin II. J Muscle Res Cell Motil 2004, 25:337-41.
18. Allingham JS, Smith R, Rayment I. The structural basis of blebbistatin inhibition and specificity for myosin II. Nat Struct Mol Biol 2005, 12:378-9.
19. Eddinger TJ, Meer DP, Miner AS, Meehl J, Rovner AS, Ratz PH. Potent inhibition of arterial smooth muscle tonic contractions by the selective myosin II inhibitor, blebbistatin. J Pharmacol Exp Ther 2007, 320:865-70.
20. Granchi S, Vannelli GB, Vignozzi L, Crescioli C, Ferruzzi P, Mancina R, Vinci MC, Forti G, Filippi S, Luconi M, Ledda F, Maggi M. Expression and regulation of endothelin-1 and its receptors in human penile smooth muscle cells. Mol Hum Reprod 2002, 8:1053-64.
21. Yamamoto M, Harm SC, Grasser WA, Thiede MA. Parathyroid hormone-related protein in the rat urinary bladder: A smooth muscle relaxant produced locally in response to mechanical stretch. Proc Natl Acad Sci USA 1992, 89:5326-30.
22. Ghasemi M, Sadeghipour H, Shafaroodi H, Nezami BG, Gholipour T, Hajrasouliha AR, Tavakoli S, Nobakht M, Moore KP, Mani AR, Dehpour AR. Role of the nitric oxide pathway and the endocannabinoid system in neurogenic relaxation of corpus cavernosum from biliary cirrhotic rats. Br J Pharmacol 2007, 151:591-601.
23. Ren J, Wang GJ, Petrovski P, Ren BH, Brown RA. Influence of hypertension on acetaldehyde-induced vasorelaxation in rat thoracic aorta. Pharmacol Res 2002, 45:195-9.
24. Sandhu KS, Chua RG, Zhang X, Kanika ND, Collins SA, Mikhail M, Melman A, DiSanto ME. Regional heterogeneity in expression of the sphingosine-1-phosphate pathway in the female rat lower urinary tract. Am J Obstet Gynecol 2009, 200:576.
25. Melman A, Biggs G, Davies K, Zhao W, Tar MT, Christ GJ. Gene transfer with a vector expressing Maxi-K from a smooth muscle-specific promoter restores erectile function in the aging rat. Gene Ther 2008, 15:364-70.
26. Yarnitsky D, Sprecher E, Barilan Y, Vardi Y. Corpus cavernosum electromyogram: Spontaneous and evoked electrical activities. J Urol 1995, 153:653-4.
27. Holmquist F, Andersson KE, Hedlund H. Effects of pinacidil on isolated human corpus cavernosum penis. Acta Physiol Scand 1990, 138:463-9.
28. Christ GJ, Maayani S, Valcic M, Melman A. Pharmacological studies of human erectile tissue: Characteristics of spontaneous contractions and alterations in alpha-adrenoceptor responsiveness with age and disease in isolated tissues. Br J Pharmacol 1990, 101:375-81.
29. Karasu C. Time course of changes in endothelium-dependent and -independent relaxation of chronically diabetic aorta: Role of reactive oxygen species. Eur J Pharmacol 2000, 392:163-73.
30. Francavilla S, Properzi G, Bellini C, Marino G, Ferri C, Santucci A. Endothelin-1 in diabetic and nondiabetic men with erectile dysfunction. J Urol 1997, 158:1770-4.
31. Khan MA, Thompson CS, Sullivan ME, Dashwood MR, Jeremy JY, Morgan RJ, Mikhailidis DP. Endothelin and erectile dysfunction: A target for pharmacological intervention?. Expert Opin Investig Drugs 1998, 7:1759-67.
32. Chang S, Hypolite JA, Changolkar A, Wein AJ, Chacko S, DiSanto ME. Increased contractility of diabetic rabbit corpora smooth muscle in response to endothelin is mediated via Rho-kinase beta. Int J Impot Res 2003, 15:53-62.
33. Carneiro FS, Nunes KP, Giachini FR, Lima VV, Carneiro ZN, Nogueira EF, Leite R, Ergul A, Rainey WE, Clinton WR, Tostes RC. Activation of the ET-1/ETA pathway contributes to erectile dysfunction associated with mineralocorticoid hypertension. J Sex Med 2008, 5:2793-807.
34. Sato Y, Zhao W, Christ GJ. Central modulation of the NO/cGMP pathway affects the MPOA-induced intracavernous pressure response. Am J Physiol Regul Integr Comp Physiol 2001, 281:R269-78.
35. Abber JC, Lue TF, Orvis BR, McClure RD, Williams RD. Diagnostic tests for impotence: A comparison of papaverine injection with the penile-brachial index and nocturnal penile tumescence monitoring. J Urol 1986, 135:923-5.