Optimization of bacterial whole cell bioreporters for toxicity assay of environmental samples

Environmental Science and Technology

Volumn 43, Issue 20, 2009, Pages 7931-7938

  Song, Yizhi ^a,b,c   Li, Guanghe ^a,c   Thornton, Steven F ^b   Thompson, Ian P ^d   Banwart, Steven A ^b   Lerner, David N ^b   Huang, Wei E ^b  

^a TSINGHUA UNIVERSITY   (China)

^b UNIVERSITY OF SHEFFIELD   (United Kingdom)

^c State Key Joint Laboratory of Environment Simulation and Pollution Control SKLESPC   (China)

^d UNIVERSITY OF OXFORD   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ACINETOBACTERS; CYTOXICITY; DETECTION LIMITS; E. COLI; ENVIRONMENTAL CONTAMINANT; ENVIRONMENTAL SAMPLE; ETHIDIUM BROMIDE; FALSE NEGATIVES; GENOTOXICITIES; HOST STRAIN; IDEAL MODEL; INDUCTION TIME; METHANESULFONATES; MITOMYCIN C; NANO-AU; NANOCARBON TUBES; NATURAL ENVIRONMENTS; PHENOLIC COMPOUNDS; REPORTER GENE; SENSING SYSTEMS; SINGLE-WALL; SOIL BACTERIUM; TOXIC COMPOUNDS; TOXICITY ASSAYS; TOXICITY DETECTION; UV LIGHT; WHOLE CELL; WHOLE-CELL BIOSENSORS;

BACTERIOLOGY; BIOLUMINESCENCE; BIOSENSORS; CHEMICAL COMPOUNDS; ESCHERICHIA COLI; GENES; GEOLOGIC MODELS; GROUNDWATER; GROUNDWATER POLLUTION; HYDROGEOLOGY; LIGHT EMISSION; METALLIC MATRIX COMPOSITES; NUCLEIC ACIDS; OPTIMIZATION; PHENOLS; PHOSPHORESCENCE; SENSORS; TOLUENE;

TOXICITY;

BENZO[A]PYRENE; DNA; ETHIDIUM BROMIDE; GOLD; GROUND WATER; HEAVY METAL; HYDROGEN PEROXIDE; MESYLIC ACID METHYL ESTER; MITOMYCIN C; PHENOL DERIVATIVE; PYRENE; SINGLE WALLED NANOTUBE; TOLUENE;

ANTIBIOTICS; BACTERIUM; BIOASSAY; BIOLUMINESCENCE; CHEMICAL ANALYSIS; ENVIRONMENTAL INDICATOR; GENOTOXICITY; GROUNDWATER POLLUTION; PHENOLIC COMPOUND; POLLUTION MONITORING; TOXIC SUBSTANCE; TOXICITY TEST;

ACINETOBACTER; ARTICLE; BIOLUMINESCENCE; BIOSENSOR; CONTROLLED STUDY; DNA DAMAGE; DNA SEQUENCE; ESCHERICHIA COLI; GENE EXPRESSION; NONHUMAN; PHOTORHABDUS LUMINESCENS; POLYMERASE CHAIN REACTION; REPORTER GENE; SOIL MICROFLORA; SOIL POLLUTION; ULTRAVIOLET IRRADIATION; WATER CONTAMINATION;

ACINETOBACTER; BENZO(A)PYRENE; BIOSENSING TECHNIQUES; DNA DAMAGE; GENES, REPORTER; HAZARDOUS SUBSTANCES; MITOMYCIN; TOXICITY TESTS; WATER POLLUTION, CHEMICAL;

ACINETOBACTER BAYLYI; BACTERIA (MICROORGANISMS); PHOTORHABDUS LUMINESCENS;

EID: 70449956719     PISSN: 0013936X     EISSN: 15205851     Source Type: Journal    
DOI: 10.1021/es901349r     Document Type: Article

References (52)

1. Schwarzenbach, R. P.; Escher, B. I.; Fenner, K.; Hofstetter, T. B.; Johnson, C. A.; von Gunten, U.; Wehrli, B. The challenge of micropollutants in aquatic systems. Science 2006, 313 (5790), 1072-1077.
2. Belkin, S. Microbial whole-cell sensing systems of environmental pollutants. Curr. Opin. Microbiol. 2003, 6 (3), 206-212. (Pubitemid 36841662)
3. Ames, B. N.; McCann, J.; Yamasaki, E. Methods for detecting carcinogens and mutagens with Salmonella-mammalian-microsome mutagenicity test. Mutat. Res. 1975, 31 (6), 347-363.
4. Maron, D. M.; Ames, B. N. Revised methods for the Salmonella mutagenicity test. Mutat. Res. 1983, 113 (3-4), 173-215.
5. McCann, J.; Choi, E.; Yamasaki, E.; Ames, B. N. Detection of carcinogens as mutagens in Salmonella microsome test - assay of 300 chemicals. Proc. Natl. Acad. Sci. U.S.A. 1975, 72 (12), 5135-5139.
6. Ames, B. N.; Durston, W. E.; Yamasaki, E.; Lee, F. D. Carcinogens are mutagens - simple test system combining liver homogenates for activation and bacteria for detection. Proc. Natl. Acad. Sci. U.S.A. 1973, 70 (8), 2281-2285.
7. Mortelmans, K.; Zeiger, E. The Ames Salmonella/microsome mutagenicity assay. Mutat. Res. 2000, 455 (1-2), 29-60.
8. Ron, E. Z. Biosensing environmental pollution. Curr. Opin. Biotechnol. 2007, 18 (3), 252-256.
9. Sorensen, S. J.; Burmolle, M.; Hansen, L. H. Making bio-sense of toxicity: new developments in whole-cell bioreporters. Curr. Opin. Biotechnol. 2006, 17 (1), 11-16.
10. Dreier, J.; Breitmaier, E. B.; Gocke, E.; Apfel, C. M.; Page, M. G. P. Direct influence of S9 liver homogenate on fluorescence signals: impactonpractical applications in a bacterial genotoxicity assay. Mutat. Res. 2002, 513 (1-2), 169-182.
11. Heitzer, A.; Malachowsky, K.; Thonnard, J. E.; Bienkowski, P. R.; White, D. C.; Sayler, G. S. Optical Bioreporter For Environmental Online Monitoring of Naphthalene and Salicylate Bioavailability With an Immobilized Bioluminescent Catabolic Reporter Bacterium. Appl. Environ. Microbiol. 1994, 60 (5), 1487-1494.
12. Huang, W. E.; Huang, L. F.; Preston, G. M.; Naylor, M.; Carr, J. P.; Li, Y. H.; Singer, A. C.; Whiteley, A. S.; Wang, H. Quantitative in situ assay of salicylic acid in tobacco leaves using a genetically modified bioreporter strain of Acinetobacter sp ADP1. Plant J. 2006, 46 (6), 1073-1083. (Pubitemid 43909266)
13. Huang, W. E.; Singer, A. C.; Spiers, A. J.; Preston, G. M.; Whiteley, A. S. Characterizing the regulation of the Pu promoter in Acinetobacter baylyi ADP1. Environ. Microbiol. 2008, 10 (7), 1668-1680. (Pubitemid 351793609)
14. Huang, W. E.; Wang, H.; Huang, L. F.; Zheng, H. J.; Singer, A. C.; Thompson, I. P.; Whiteley, A. S. Chromosomally located gene fusions constructed in Acinetobacter sp. ADP1 for the environmental detection of salicylate. Environ. Microbiol. 2005, 7 (9), 1339-1348.
15. King, J. M. H.; Digrazia, P. M.; Applegate, B.; Burlage, R.; Sanseverino, J.; Dunbar, P.; Larimer, F.; Sayler, G. S. Rapid, Sensitive Bioluminescent Reporter Technology For Naphthalene Exposure and Biodegradation. Science 1990, 249 (4970), 778-781. (Pubitemid 20259113)
16. Davidov, Y.; Rozen, R.; Smulski, D. R.; Van Dyk, T. K.; Vollmer, A. C.; Elsemore, D. A.; LaRossa, R. A.; Belkin, S. Improved bacterial SOS promoter:: lux fusions for genotoxicity detection. Mutat. Res. 2000, 466 (1), 97-107. (Pubitemid 30177144)
17. Norman, A.; Hansen, L. H.; Sorensen, S. J. A flow cytometry-optimized assay using an SOS-green fluorescent protein (SOSGFP) whole-cell bioreporter for the detection of genotoxins in complex environments. Mutat. Res. 2006, 603 (2), 164-172.
18. Rogers, K. R. Recent advances in bioreporter techniques for environmental monitoring. Anal. Chim. Acta 2006, 568 (1-2), 222-231. (Pubitemid 43729735)
19. Rusling, J. F.; Hvastkovs, E. G.; Schenkman, J. B. Toxicity screening using bioreporters that measure DNA damage. Curr. Opin. Drug Discovery Dev. 2007, 10 (1), 67-73.
20. Ptitsyn, L. R.; Horneck, G.; Komova, O.; Kozubek, S.; Krasavin, E. A.; Bonev, M.; Rettberg, P. A bioreporter for environmental genotoxin screening based on an SOS lux assay in recombinant Escherichia coli cells. Appl. Environ. Microbiol. 1997, 63 (11), 4377-4384. (Pubitemid 27485764)
21. Pickup, R. W.; Mallinson, H. E. H.; Rhodes, G.; Alamillo, M. L.; Thornton, S. F.; Lerner, D. N. In A phenol-contaminated groundwater site: a microbiological perspective, 3rd International Conference on Groundwater Quality, Sheffield, England, Jun 18-21, 2001; Thornton, S. F.; Oswald, S. E., Eds.; Sheffield, England, 2001; pp 251-256.
22. Thornton, S. F.; Quigley, S.; Spence, M. J.; Banwart, S. A.; Bottrell, S.; Lerner, D. N. Processes controlling the distribution and natural attenuation of dissolved phenolic compounds in a deep sandstone aquifer. J. Contam. Hydrol. 2001, 53 (3-4), 233-267. (Pubitemid 33129995)
23. Merschsundermann, V.; Mochayedi, S.; Kevekordes, S. Genotoxicity of polycyclic aromatic-hydrocarbons in Escherichia-coli PQ37. Mutat. Res. 1992, 278 (1), 1-9.
24. Thornton, S. F.; Quigley, S.; Spence, M. J.; Banwart, S. A.; Bottrell, S.; Lerner, D. N. Processes controlling the distribution and natural attenuation of dissolved phenolic compounds in a deep sandstone aquifer. J. Contam. Hydrol. 2001, 53, 233-267. (Pubitemid 33129995)
25. Tuxen, N.; Albrechtsen, H.-J.; Bjerg, P. L. Identification of a reactive fringe zone at a landfill leachate plume fringe using high-resolution sampling and incubation techniques. J. Contam. Hydrol. 2006, 85, 179-194.
26. Lerner, D. N.; Thornton, S. F.; Spence, M. J.; Banwart, S. A.; Bottrell, S. H.; Higgo, J. J.; Mallinson, H. E. H.; Pickup, R. W.; Williams, G. M. Ineffective natural attenuation of degradable organic compounds in a phenol-contaminated aquifer. Ground Water 2000, 38 (6), 922-928. (Pubitemid 32450119)
27. Brambilla, G.; Martelli, A. Update on genotoxicity and carcinogenicity testing of 472 marketed pharmaceuticals. Mutat. Res. 2009, 281 (2-3), 209-229.
28. Mohn, G. R. Bacterial systems for carcinogenicity testing. Mutat. Res. 1981, 87 (2), 191-210.
29. Rauch, P. J. G.; Palmen, R.; Burds, A. A.; GreggJolly, L. A.; vanderZee, J. R.; Hellingwerf, K. J. The expression of the Acinetobacter calcoaceticus recA gene increases in response to DNA damage independently of RecA and of development of competence for natural transformation. Microbiology (Reading, U. K.) 1996, 142, 1025-1032.
30. Hare, J. M.; Perkins, S. N.; Gregg-Jolly, L. A. A constitutively expressed, truncated umuDC operon regulates the recA-dependent DNA damage induction of a gene in Acinetobacter baylyi strain ADP1. Appl. Environ. Microbiol. 2006, 72 (6), 4036-4043.
31. Greggjolly, L. A.; Ornston, L. N. Properties of Acinetobacter- Calcoaceticus Reca and Its Contribution to Intracellular Gene Conversion. Mol. Microbiol. 1994, 12 (6), 985-992.
32. Barbe, V.; Vallenet, D.; Fonknechten, N.; Kreimeyer, A.; Oztas, S.; Labarre, L.; Cruveiller, S.; Robert, C.; Duprat, S.; Wincker, P.; Ornston, L. N.; Weissenbach, J.; Marliere, P.; Cohen, G. N.; Medigue, C. Unique features revealed by the genome sequence of Acinetobacter sp ADP1, a versatile and naturally transformation competent bacterium. Nucleic Acids Res. 2004, 32 (19), 5766-5779. (Pubitemid 39619160)
33. Lee, H. J.; Villaume, J.; Cullen, D. C.; Kim, B. C.; Gu, M. B. In Monitoring and classification of PAH toxicity using an immobilized bioluminescent bacteria, 7th World Congress on Bioreporters, Kyoto, Japan, May 15-17, 2002; Kyoto, Japan, 2002; pp 571-577.
34. Lee, J. H.; Mitchell, R. J.; Kim, B. C.; Cullen, D. C.; Gu, M. B. A cell array bioreporter for environmental toxicity analysis. Biosens. Bioelectron. 2005, 21 (3), 500-507. (Pubitemid 41074938)
35. Min, J. H.; Kim, E. J.; LaRossa, R. A.; Gu, M. B. Distinct responses of a recA:: luxCDABE Escherichia coli strain to direct and indirect DNA damaging agents. Mutat. Res. 1999, 442 (2), 61-68.
36. Vollmer, A. C.; Belkin, S.; Smulski, D. R.; VanDyk, T. K.; LaRossa, R. A. Detection of DNA damage by use of Escherichia coli carrying recA'-lux, uvrA'-lux, or alkA'-lux reporter plasmids. Appl. Environ. Microbiol. 1997, 63 (7), 2566-2571.
37. Elad, T.; Benovich, E.; Magrisso, S.; Belkin, S. Toxicant Identification by a Luminescent Bacterial Bioreporter Panel: Application of Pattern Classification Algorithms. Environ. Sci. Technol. 2008, 42 (22), 8486-8491.
38. Tyo, K, E. J.; Ajikumar, K. P.; Stepanauskas, R. Stabilized gene duplication enables long-term selection-free heterologous pathway expression. Nat. Biotechnol. 2009, 27 (8), 760-765.
39. Baumann, P. Isolation of Acinetobacter from soil and water. J. Bacteriol. 1968, 96, 39-42.
40. Young, D. M.; Parke, D.; Ornston, L. N. Opportunities for Genetic Investigation Afforded by Acinetobacter baylyi, A Nutritionally Versatile Bacterial Species that is Highly Competent for Natural Transformation. Annu. Rev. Microbiol. 2005, 59, 519-551.
41. Hakkila, K.; Maksimow, M.; Karp, M.; Virta, M. Reporter genes lucFF, luxCDABE, gfp, and dsred have different characteristics in whole-cell bacterial sensors. Anal. Biochem. 2002, 301 (2), 235-242. (Pubitemid 34158188)
42. Sagi, E.; Hever, N.; Rosen, R.; Bartolome, A. J.; Premkumar, J. R.; Ulber, R.; Lev, O.; Scheper, T.; Belkin, S. Fluorescence and bioluminescence reporter functions in genetically modified bacterial sensor strains. Sens. Actuators, B 2003, 90, 2-8.
43. Winson, M. K.; Swift, S.; Fish, L.; Throup, J. P.; Jorgensen, F.; Chhabra, S. R.; Bycroft, B. W.; Williams, P.; Stewart, G. Construction and analysis of luxCDABE-based plasmid sensors for investigating N-acyl homoserine lactone-mediated quorum sensing. FEMS Microbiol. Lett. 1998, 163 (2), 185-192.
44. Winson, M. K.; Swift, S.; Hill, P. J.; Sims, C. M.; Griesmayr, G.; Bycroft, B. W.; Williams, P.; Stewart, G. Engineering the luxCDABE genes from Photorhabdus luminescens to provide a bioluminescent reporter for constitutive and promoter probe plasmids and mini-Tn5 constructs. FEMS Microbiol. Lett. 1998, 163 (2), 193-202. (Pubitemid 28289358)
45. Bjerketorp, J.; Hakansson, S.; Belkin, S.; Jansson, J. K. Advances in preservation methods: keeping bioreporter microorganisms alive and active. Curr. Opin. Biotechnol. 2006, 17 (1), 43-49.
46. Juhasz, A. L.; Naidu, R. Bioremediation of high molecular weight polycyclic aromatic hydrocarbons: a review of the microbial degradation of benzo[a]pyrene. Int. Biodeterior. Biodegrad. 2000, 45 (1-2), 57-88. (Pubitemid 30629048)
47. Sram, R. J.; Binkova, B.; Rossner, P.; Rubes, J.; Topinka, J.; Dejmek, J. In Adverse reproductive outcomes from exposure to environmental mutagens, 3rd International Conference on Environmental Mutagens in Human Populations, Bangkok, Thailand, Nov 28-Dec 04, 1998; Bangkok, Thailand, 1998; pp 203-215.
48. Shimada, T.; Fujii-Kuriyama, Y. Metabolic activation of polycyclic aromatic hydrocarbons to carcinogens by cytochromes P450 1A1 and 1B1. Cancer Sci. 2004, 95 (1), 1-6. (Pubitemid 38208399)
49. Conney, A. H. Induction of microsomal-enzymes by foreign chemicals and carcinogenesis by polycyclic aromatic hydrocarbons. Cancer Sci. 1982, 42 (12), 4875-4917.
50. Shimizu, Y.; Nakatsuru, Y.; Ichinose, M.; Takahashi, Y.; Kume, H.; Mimura, J.; Fujii-Kuriyama, Y.; Ishikawa, T. Benzo[a]pyrene carcinogenicity is lost in mice lacking the aryl hydrocarbon receptor. Proc. Natl. Acad. Sci. U.S.A. 2000, 97 (2), 779-782.
51. Juni, E.; Janik, A. Transformation of Acinetobacter calcoaceticus (Bacterium anitratum). J. Bacteriol. 1969, 98, 281-288.
52. Verschaeve, L.; Van Gompel, J.; Thilemans, L.; Regniers, L.; Vanparys, P.; van der Lelie, D. VITOTOX(R) bacterial genotoxicity and toxicity test for the rapid screening of chemicals. Environ. Mol. Mutagen. 1999, 33 (3), 240-248.