Construction and analysis of luxCDABE-based plasmid sensors for investigating N-acyl homoserine lactone-mediated quorum sensing

FEMS Microbiology Letters

Volumn 163, Issue 2, 1998, Pages 185-192

  Winson, Michael K ^a,b   Swift, Simon ^a,b   Fish, Leigh ^a,b   Throup, John P ^a,b   Jørgensen, Frieda ^a   Chhabra, Siri Ram ^b   Bycroft, Barrie W ^b   Williams, Paul ^b   Stewart, Gordon S A B ^a  

^a Food Microbiology Section   (United Kingdom)

^b UNIVERSITY OF NOTTINGHAM   (United Kingdom)

Author keywords

Bioluminescence; Lux; N Acyl homoserine lactone; Quorum sensing

Indexed keywords

HOMOSERINE; LACTONE;

ANALYTIC METHOD; ARTICLE; BACTERIAL VIRULENCE; BACTERIUM EXAMINATION; BIOLUMINESCENCE; CELL POPULATION; LIGAND BINDING; NONHUMAN; PLASMID; PRIORITY JOURNAL; SENSOR; STRUCTURE ACTIVITY RELATION;

4-BUTYROLACTONE; BACTERIAL PROTEINS; CONJUGATION, GENETIC; DNA-BINDING PROTEINS; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, BACTERIAL; GENES, REPORTER; GENETIC VECTORS; LUMINESCENT MEASUREMENTS; PLASMIDS; REPRESSOR PROTEINS; SIGNAL TRANSDUCTION; STRUCTURE-ACTIVITY RELATIONSHIP; TRANS-ACTIVATORS;

BACTERIA (MICROORGANISMS); NEGIBACTERIA;

EID: 0032526689     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(98)00172-4     Document Type: Article

References (20)

1. Swift, S., Throup, J.P., Salmond, G.P.C., Williams, P. and Stewart, G.S.A.B. (1996) Quorum sensing: a population-density component in the determination of bacterial phenotype. Trends Biochem. Sci. 21, 214-219.
2. Chhabra, S.R., Stead, P., Bainton, N.J., Salmond, G.P.C., Williams, P., Stewart, G.S.A.B. and Bycroft, B.W. (1993) Autoregulation of carbapenem biosynthesis in Erwinia carotovora ATCC 39048 by analogues of N-(3-oxohexanoyl)-L-homoserine lactone. J. Antibiot. 46, 441-454.
3. Schaefer, A.L., Hanzelka, B.L., Eberhard, A. and Greenberg, E.P. (1996) Quorum sensing in Vibrio fischeri: probing autoinducer-LuxR interactions with autoinducer analogs. J. Bacteriol. 178, 2897-2901.
4. Passador, L., Tucker K.D., Guertin, K.R., Journet, M.P., Kende, A.S. and Iglewski B.H. (1996) Functional analysis of the Pseudomonas aeruginosa autoinducer PAI. J. Bacteriol. 178, 5995-6000.
5. Winson, M.K., Camara, M., Latifi, A., Foglino, M., Chhabra, S.R., Daykin, M., Chapon, V., Bycroft, B.W., Salmond, G.P.C., Lazdunski, A., Stewart, G.S.A.B. and Williams, P. (1995) Multiple N-acyl-L-homoserine lactone signal molecules regulate production of virulence determinants and secondary metabolites in Pseudomonas aeruginosa. Proc. Natl. Acad. Sci. USA 92, 9427-9431.
6. Latifi, A., Winson, M.K., Foglino, M., Bycroft, B.W., Stewart, G.S.A.B., Lazdunski, A. and Williams, P. (1995) Multiple homologues of LuxR and LuxI control expression of virulence determinants and secondary metabolites through quorum sensing in Pseudomonas aeruginosa PA01. Mol. Microbiol. 17, 333-343.
7. Swift, S., Winson, M.K., Chan, P.F., Bainton, N.J., Birdsall, M., Reeves, P.J., Rees, C.E.D., Chhabra, S.R., Hill, P.J., Throup, J.P., Bycroft, B.W., Salmond, G.P.C., Williams, P. and Stewart, G.S.A.B. (1993) A novel strategy for the isolation of luxI homologues: evidence for the widespread distribution of LuxR:LuxI superfamily in enteric bacteria. Mol. Microbiol. 10, 511-520.
8. Throup, J.P., Camara, M., Briggs, G.S., Winson, M.K., Chhabra, S.R., Bycroft, B.W., Williams, P. and Stewart, G.S.A.B. (1995) Characterization of the yenI/yenR locus from Yersinia entercolitica mediating the synthesis of two N-acylhomoserine lactone signal molecules. Mol. Microbiol. 17, 345-356.
9. Eberl, L., Winson, M.K., Sternberg, C., Stewart, G.S.A.B., Christiansen, G., Chhabra, S.R., Bycroft, B.W., Williams, P., Molin, S. and Givskov, M. (1996) Involvement of N-acyl-L-homoserine lactone autoinducers in controlling the multicellular behaviour of Serratia liquefaciens. Mol. Microbiol. 20, 127-136.
10. Swift, S., Karlyshev, A.V., Fish, L., Durant, E.L., Winson, M.K., Chhabra, S.R., Williams, P., Macintyre, S. and Stewart, G.S.A.B. (1997) Quorum sensing in Aeromonas hydrophila and Aeromonas salmonicida: Identification of the LuxRI homologues AhyRI and AsaRI and their cognate N-acylhomoserine lactone signal molecules. J. Bacteriol. 179, 5271-5281.
11. Piper, K.R., Beck von Bodman, S. and Farrand, S.K. (1993) Conjugation factor of Agrobacterium tumefaciens regulates Ti plasmid transfer by autoinduction. Nature 362, 448-450.
12. Pesci, E.P., Pearson, J.P., Seed, P.C. and Iglewski, B.H. (1997) Regulation of las and rhl quorum sensing in Pseudomonas aeruginosa. J. Bacteriol. 179, 3127-3132.
13. Shaw, P.D., Ping, G., Daly, S.L., Cha, C., Cronan Jr., J.E., Rinehart, K.L. and Farrand, S.K. (1997) Detecting and characterizing N-acyl-homoserine lactone signal molecules by thin layer chromatography. Proc. Natl. Acad. Sci. USA 94, 6036-6041.
14. Winson, M.K., Swift, S., Hill, P.J., Sims, C.M., Griesmayr, G., Bycroft, B.W., Williams, P. and Stewart, G.S.A.B. Engineering the luxCDABE genes from Photorhabdus luminescens to provide a bioluminescent reporter for constitutive and promoter probe plasmids and mini-Tn5 constructs. Submitted.
15. Yanisch-Peron, C., Vieira, J. and Messing, J. (1985) Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33, 103-109.
16. Herrero, M., de Lorenzo, V. and Timmis, K.N. (1990) Transposon vectors containing non-antibiotic resistance selection markers for cloning and stable chromosomal insertion of foreign genes in Gram-negative bacteria. J. Bacteriol. 172, 6557-6567.
17. Simon, R., Priefer, U. and Pühler, A. (1983) A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in Gram-negative bacteria. Biotechnology 1, 784-791.
18. Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A. and Struhl, K. (1989) Short Protocols in Molecular Biology. John Wiley, Chichester.
19. Keen, N.T., Tamaki, S., Kobayashi, D. and Trollinger, D. (1988) Improved broad-host range plasmids for DNA cloning in Gram-negative bacteria. Gene 70, 191-197.
20. Schweizer, H.P. (1991) Escherichia-Pseudomonas shuttle vectors derived from pUC18/19. Gene 97, 109-112.