Purification, characterization and n-terminal amino acid sequencing of sakacin 1, A bacteriocin produced by Lactobacillus sakei 1

Journal of Food Safety

Volumn 29, Issue 4, 2009, Pages 636-649

  D'Angelis, C E M ^a,b   Polizello, A C M ^a   Nonato, M C ^a   Spadaro, A C C ^a   De Martinis, E C P ^a  

^a UNIVERSITY OF SÃO PAULO   (Brazil)

^b Faculdades Integradas Pitágoras de Montes Claros   (Brazil)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIA (MICROORGANISMS); LACTOBACILLUS SAKEI; LISTERIA MONOCYTOGENES;

EID: 70449130791     PISSN: 01496085     EISSN: 17454565     Source Type: Journal    
DOI: 10.1111/j.1745-4565.2009.00183.x     Document Type: Article

References (28)

1. ALVES, V.F., LAVRADOR, M.A.S. DE MARTINIS, E.C.P. 2003. Bacteriocin exposure and food ingredients influence on growth and virulence of Listeria monocytogenes in a model meat gravy system. J. Food Saf. 23, 201 217.
2. ALVES, V.F., MARTINEZ, R.C.R., LAVRADOR, M.A.S. DE MARTINIS, E.C.P. 2006. Antilisterial activity of lactic acid bacteria inoculated on cooked ham. Meat Sci. 74, 623 627.
3. BRADFORD, M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248 254.
4. CAROLISSEN-MACKAY, V., ARENDSE, G. HASTINGS, J.W. 1997. Purification of bacteriocins of lactic acid bacteria: Problems and pointers. Int. J. Food Microbiol. 34, 1 16.
5. CHUMCHALOVÁ, J., STILES, J., JOSEPHSEN, J. PLOCKOVÁ, M. 2004. Characterization of acidocin CH5, a bacteriocin produced by Lactobacillus acidophilus CH5. J. Appl. Microbiol. 96, 1082 1089.
6. COTTER, P.D., HILL, C. ROSS, R.P. 2005. Bacteriocins: Developing innate immunity for food. Nat. Rev. Microbiol. 3, 777 788.
7. DE MARTINIS, E.C.P. 2002. Identification of meat isolated bacteriocin-producing lactic acid bacteria using biotyping and ribotyping. Arq. Bra. Med. Vet. Zootec. 54, 659 661.
8. DE MARTINIS, E.C.P., PÚBLIO, M.R.P., SANTAROSA, P.R. FREITAS, F.Z. 2001. Antilisterial activity of lactic acid bacteria isolated from vacuum-packaged Brazilian meat and meat products. Braz. J. Microbiol. 32, 32 37.
9. DE MARTINIS, E.C.P., ALVES, V.F. FRANCO, B.D.G. 2002. Fundamentals and perspectives for the use of bacteriocins produced by lactic acid bacteria in meat products. Food Rev. Int. 18, 191 208.
10. DE MARTINIS, E.C.P., SANTAROSA, P.R. FREITAS, F.Z. 2003. Caracterização preliminar de bacteriocinas produzidas por seis cepas de bactérias láticas isoladas de produtos cárneos embalados a vácuo. Ciência e Tecnologia de Alimentos 23, 195 199.
11. EIJSINK, V.G.H., SKEIE, M., MIDDELHOVEN, P.H., BRURBERG, M.B. NES, I.F. 1998. Comparative studies of class IIa bacteriocins of lactic acid bacteria. Appl. Environ. Microbiol. 64, 3275 3281.
12. ENNAHAR, S., SASHIHARA, T., SONOMOTO, K. ISHIZAKI, A. 2000. Class IIa bacteriocins: Biosynthesis, structure and activity. FEMS Microbiol. Rev. 24, 85 106.
13. GIBBS, G.M., DAVIDSON, B.E. HILLIER, A.J. 2004. Novel expression system for large-scale production and purification of recombinant class IIa bacteriocins and its application to piscicolin 126. Appl. Environ. Microbiol. 70, 3292 3297.
14. HEUKESHOVEN, J. DERNICK, R. 1985. Simplified method for silver staining of proteins in polyacrylamide gels and the mechanism of silver staining. Electrophoresis 6, 103 112.
15. JACK, R.W. TAGG, J.R. 1992. Factors affecting production of the group A streptococcus bacteriocin SA-FF22. J. Microbiol. 30, 132 138.
16. LAEMMLI, U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680 685.
17. LARSEN, A.G., VOGENSEN, F.K. JOSEPHSEN, J. 1993. Antimicrobial activity of lactic acid bacteria isolated from sourdoughs: Purification and characterization of bavaricin A, a bacteriocin produced by Lactobacillus bavaricus MI401. J. Appl. Bacteriol. 75, 113 122.
18. MATARAGAS, M., DROSINOS, E.H., TSAKALIDOU, E. METAXOPOULOS, J. 2004. Influence of nutrients on growth and bacteriocin production by Leuconostoc mesenteroides L124 and Lactobacillus curvatus L442. Antonie van Leeuwenhoek 85, 191 198.
19. MAYR-HARTING, A., HEDGES, A.J. BERKELEY, R.C.W. 1972. Methods for studying bacteriocins. In Methods in Microbiology (J.R. Norris D.W. Ribbons, eds.) pp. 313 342. Academic Press, New York, NY.
20. MØRTVEDT, C.I., NISSEN-MEYER, J., SLETTEN, K. NES, I.F. 1991. Purification and amino acid sequence of lactocin S, a bacteriocin produced by Lactobacillus sakei L45. Appl. Environ. Microbiol. 57, 1829 1834.
21. NILSEN, T., NES, I.F. HOLO, H. 1998. An exported inducer peptide regulates bacteriocin production in Enterococcus faecium CTC 492. J. Bacteriol. 180, 1848 1854.
22. PARENTE, E. RICCIARDI, A. 1999. Production of bacteriocins from lactic acid bacteria. Appl. Microbiol. Biotechnol. 52, 628 638.
23. ROSA, C.M., FRANCO, B.D.G.M., MONTVILLE, T.J. CHIKINDAS, M.L. 2002. Purification and mechanistic action of a bacteriocin produced by a Brazilian sausage isolate, Lactobacillus sakei 2a. J. Food Saf. 22, 39 54.
24. UTENG, M., HAUGE, H.H., BRONDZ, I., NISSEN-MEYER, J. FIMLAND, G. 2002. Rapid two-step procedure for large-scale purification of pediocin-like bacteriocins and other cationic antimicrobial peptides from complex culture medium. Appl. Environ. Microbiol. 68, 952 956.
25. UTENG, M., HAUGE, H.H., MARKWICK, P.R.L., FIMLAND, G., MANTZILAS, D., NISSEN-MEYER, J. MUHLE-GOLL, C. 2003. Three-dimensional structure in lipid micelles of the pediocin-like antimicrobial peptide sakacin P and sakacin P variant that is structurally stabilized by an inserted C-terminal disulfide bridge. Biochemistry 42, 11417 11426.
26. VAUGHAN, A., EIJSINK, V.G.H., O'SULLVIAN, T.F., O'HANLON, K. VAN SIDEREN, D. 2001. An analysis of bacteriocins produced by lactic acid bacteria from malted barley. J. Appl. Microbiol. 91, 131 138.
27. YAMAZAKI, K., SUZUKI, M., KAWAI, Y., INOUE, N. MONTVILLE, T.J. 2005. Purification and characterization of a novel class IIa bacteriocin, piscicocin CS526, from surimi-associated Carnobacterium piscicola CS526. Appl. Environ. Microbiol. 71, 554 557.
28. YANG, R., JOHNSON, M.C. RAY, B. 1992. Novel method to extract large amounts of bacteriocins from lactic acid bacteria. Appl. Environ. Microbiol. 58, 3355 3359.