Mariner MOS1 transposase optimization by rational mutagenesis

Genetica

Volumn 137, Issue 3, 2009, Pages 265-276

  Germon, Stéphanie ^a,d   Bouchet, Nicolas ^a,b   Casteret, Sophie ^a,b   Carpentier, Guillaume ^a,b   Adet, Jérémy ^a,b   Bigot, Yves ^b,c   Augé Gouillou, Corinne ^a,b  

^a UNIVERSITÉ DE TOURS   (France)

^b CNRS   (France)

^c UNIVERSITY HOSPITAL OF TOURS   (France)

^d CEMAGREF   (France)

Author keywords

Hyperactive transposases; Mariner transposases; Mos1 element; Phosphorylation; Rational mutagenesis; Transposon tools

Indexed keywords

DNA BINDING PROTEIN; MARINER TRANSPOSASES; MUTANT PROTEIN; TRANSPOSASE;

ARTICLE; CALIBRATION; ENZYME ACTIVATION; GENE EXPRESSION REGULATION; GENETICS; HELA CELL; HUMAN; METABOLISM; METHODOLOGY; PHYSIOLOGY; PROTEIN DOMAIN; PROTEIN ENGINEERING; PROTEIN MOTIF; PROTEIN MULTIMERIZATION; STANDARD;

AMINO ACID MOTIFS; CALIBRATION; DNA-BINDING PROTEINS; ENZYME ACTIVATION; HELA CELLS; HUMANS; MUTAGENESIS, INSERTIONAL; MUTANT PROTEINS; PROTEIN ENGINEERING; PROTEIN INTERACTION DOMAINS AND MOTIFS; PROTEIN MULTIMERIZATION; TRANSPOSASES;

ANIMALIA; BACTERIA (MICROORGANISMS); HEXAPODA; MAMMALIA;

EID: 70350707498     PISSN: 00166707     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10709-009-9375-x     Document Type: Article

References (32)

1. Auge-Gouillou C, Hamelin MH et al (2001) The ITR binding domain of the mariner Mos-1 transposase. Mol Genet Genomics 265(1):58-65. doi:10.1007/s004380000386.
2. Auge-Gouillou C, Brillet B et al (2005a) Mariner Mos1 transposase dimerizes prior to ITR binding. J Mol Biol 351(1):117-130. doi:10.1016/j.jmb.2005.05.019.
3. Auge-Gouillou C, Brillet B et al (2005b) Assembly of the mariner Mos1 synaptic complex. Mol Cell Biol 25(7):2861-2870. doi:10.1128/MCB.25.7.2861-2870.2005.
4. Baus J, Liu L et al (2005) Hyperactive transposase mutants of the sleeping beauty transposon. Mol Ther 12(6):1148-1156. doi:10.1016/j.ymthe.2005.06.484.
5. Beall EL, Mahoney MB et al (2002) Identification and analysis of a hyperactive mutant form of Drosophila P-element transposase. Genetics 162(1):217-227.
6. Bischerour J, Chalmers R (2007) Base-flipping dynamics in a DNA hairpin processing reaction. Nucleic Acids Res 35(8):2584-2595. doi:10.1093/nar/gkm186.
7. Blom N, Gammeltoft S et al (1999) Sequence and structure-based prediction of eukaryotic protein phosphorylation sites. J Mol Biol 294(5):1351-1362. doi:10.1006/jmbi.1999.3310.
8. Blom N, Sicheritz-Ponten T et al (2004) Prediction of post-translational glycosylation and phosphorylation of proteins from the amino acid sequence. Proteomics 4(6):1633-1649. doi:10.1002/pmic.200300771.
9. Brillet B (2006) Activité de la transposase de l'élément mariner Mos1: mise en place du complexe synaptique et régulation par phosphorylation. PhD of the University of Tours.
10. Brillet B, Bigot Y et al (2007) Assembly of the Tc1 and mariner transposition initiation complexes depends on the origins of their transposase DNA binding domains. Genetica 130(2):105-120. doi:10.1007/s10709-006-0025-2.
11. Butler MG, Chakraborty SA et al (2006) The N-terminus of Himar1 mariner transposase mediates multiple activities during transposition. Genetica 127(1-3):351-366. doi:10.1007/s10709-006-6250-x.
12. Dai J, Xie W et al (2007) Phosphorylation regulates integration of the yeast Ty5 retrotransposon into heterochromatin. Mol Cell 27(2):289-299. doi:10.1016/j.molcel.2007.06.010.
13. Dalsgaard T, Moldt B et al (2009) Shielding of sleeping beauty DNA transposon-delivered transgene cassettes by heterologous insulators in early embryonal cells. Mol Ther 17(1):121-130. doi:10.1038/mt.2008.224.
14. Dodd IB, Egan JB (1990) Improved detection of helix-turn-helix DNA-binding motifs in protein sequences. Nucleic Acids Res 18(17):5019-5026. doi:10.1093/nar/18.17.5019.
15. Garrison BS, Yant SR et al (2007) Postintegrative gene silencing within the sleeping beauty transposition system. Mol Cell Biol 27(24):8824-8833. doi:10.1128/MCB.00498-07.
16. Keravala A, Liu D et al (2006) Hyperactive Himar1 transposase mediates transposition in cell culture and enhances gene expression in vivo. Hum Gene Ther 17(10):1006-1018. doi:10.1089/hum.2006.17.1006.
17. Lampe DJ, Akerley BJ et al (1999) Hyperactive transposase mutants of the Himar1 mariner transposon. Proc Natl Acad Sci USA 96(20):11428-11433. doi:10.1073/pnas.96.20.11428.
18. Pledger DW, Coates CJ (2005) Mutant Mos1 mariner transposons are hyperactive in Aedes aegypti. Insect Biochem Mol Biol 35(10):1199-1207. doi:10.1016/j.ibmb.2005.06.002.
19. Reznikoff WS (2006) Tn5 transposition: a molecular tool for studying protein structure-function. Biochem Soc Trans 34(Pt 2):320-323. doi:10.1042/BST20060320.
20. Richardson JM, Zhang L et al (2004) Expression, purification and preliminary crystallographic studies of a single-point mutant of Mos1 mariner transposase. Acta Crystallogr D Biol Crystallogr 60(Pt 5):962-964. doi:10.1107/S0907444904003798.
21. Richardson JM, Dawson A et al (2006) Mechanism of Mos1 transposition: insights from structural analysis. EMBO J 25(6):1324-1334. doi:10.1038/sj.emboj.7601018.
22. Robert V, Bessereau JL (2007) Targeted engineering of the Caenorhabditis elegans genome following Mos1-triggered chromosomal breaks. EMBO J 26(1):170-183. doi:10.1038/sj.emboj.7601463.
23. Shao H, Tu Z (2001) Expanding the diversity of the IS630-Tc1-mariner superfamily: discovery of a unique DD37E transposon and reclassification of the DD37D and DD39D transposons. Genetics 159(3):1103-1115.
24. Sinzelle L, Jegot G et al (2008) Factors acting on Mos1 transposition efficiency. BMC Mol Biol 9(1):106. doi:10.1186/1471-2199-9-106.
25. Twining SS, Goryshin IY et al (2001) Functional characterization of arginine 30, lysine 40, and arginine 62 in Tn5 transposase. J Biol Chem 276(25):23135-23143. doi:10.1074/jbc.M010748200.
26. Williams DC, Boulin T et al (2005) Characterization of Mos1-mediated mutagenesis in Caenorhabditis elegans: a method for the rapid identification of mutated genes. Genetics 169(3):1779-1785. doi:10.1534/genetics.104.038265.
27. Wu SC, Meir YJ et al (2006) piggyBac is a flexible and highly active transposon as compared to sleeping beauty, Tol2, and Mos1 in mammalian cells. Proc Natl Acad Sci USA 103(41):15008-15013. doi:10.1073/pnas.0606979103.
28. Xue Y, Zhou F et al (2005) GPS: a comprehensive www server for phosphorylation sites prediction. Nucleic Acids Res 33(Web Server issue):W184-187.
29. Yant SR, Park J et al (2004) Mutational analysis of the N-terminal DNA-binding domain of sleeping beauty transposase: critical residues for DNA binding and hyperactivity in mammalian cells. Mol Cell Biol 24(20):9239-9247. doi:10.1128/MCB.24.20.9239-9247.2004.
30. Zayed H, Izsvak Z et al (2004) Development of hyperactive sleeping beauty transposon vectors by mutational analysis. Mol Ther 9(2):292-304. doi:10.1016/j.ymthe.2003.11.024.
31. Zhang L, Dawson A et al (2001) DNA-binding activity and subunit interaction of the mariner transposase. Nucleic Acids Res 29(17):3566-3575. doi:10.1093/nar/29.17.3566.
32. Zhou FF, Xue Y et al (2004) GPS: a novel group-based phosphorylation predicting and scoring method. Biochem Biophys Res Commun 325(4):1443-1448. doi:10.1016/j.bbrc.2004.11.001.