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Volumn 37, Issue 18, 2009, Pages

TrimerDimer: An oligonucleotide-based saturation mutagenesis approach that removes redundant and stop codons

Author keywords

[No Author keywords available]

Indexed keywords

9 FLUORENYLMETHYL CHLOROFORMATE; OLIGONUCLEOTIDE; GREEN FLUORESCENT PROTEIN; GREEN FLUORESCENT PROTEIN, AEQUOREA VICTORIA; ORGANOPHOSPHORUS COMPOUND; PHOSPHORAMIDOUS ACID;

EID: 70350699428     PISSN: 03051048     EISSN: 13624962     Source Type: Journal    
DOI: 10.1093/nar/gkp602     Document Type: Article
Times cited : (16)

References (28)
  • 1
    • 2542563521 scopus 로고    scopus 로고
    • Chemical and biochemical strategies for the randomization of protein encoding DNA sequences: Library construction methods for directed evolution
    • Neylon,C. (2004) Chemical and biochemical strategies for the randomization of protein encoding DNA sequences: Library construction methods for directed evolution. Nucleic Acids Res, 32, 1448-1459.
    • (2004) Nucleic Acids Res , vol.32 , pp. 1448-1459
    • Neylon, C.1
  • 2
    • 85045502002 scopus 로고
    • Randomization of genes by PCR mutagenesis
    • Cadwell,R.C. and Joyce,G.F. (1992) Randomization of genes by PCR mutagenesis. PCR Methods Appl., 2, 28-33.
    • (1992) PCR Methods Appl , vol.2 , pp. 28-33
    • Cadwell, R.C.1    Joyce, G.F.2
  • 3
    • 0022912910 scopus 로고
    • A simple and efficient procedure for saturation mutagenesis using mixed oligo-deoxynucleotides
    • Derbyshire,K.M., Salvo,J.J. and Grindley,N.D. (1986) A simple and efficient procedure for saturation mutagenesis using mixed oligo-deoxynucleotides. Gene, 46, 145-152.
    • (1986) Gene , vol.46 , pp. 145-152
    • Derbyshire, K.M.1    Salvo, J.J.2    Grindley, N.D.3
  • 4
    • 0022462026 scopus 로고
    • A complete library of point substitution mutations in the glucocorticoid response element of mouse mammary tumor virus
    • Hutchison,C.A. III, Nordeen,S.K., Vogt,K. and Edgell,M.H. (1986) A complete library of point substitution mutations in the glucocorticoid response element of mouse mammary tumor virus. Proc. Natl Acad. Sci. USA, 83, 710-714.
    • (1986) Proc. Natl Acad. Sci. USA , vol.83 , pp. 710-714
    • Hutchison III, C.A.1    Nordeen, S.K.2    Vogt, K.3    Edgell, M.H.4
  • 5
    • 0023037143 scopus 로고
    • Advantages to mutagenesis techniques generating populations containing the complete spectrum of single codon changes
    • Sirotkin,K. (1986) Advantages to mutagenesis techniques generating populations containing the complete spectrum of single codon changes. J. Theor. Biol., 123, 261-279.
    • (1986) J. Theor. Biol , vol.123 , pp. 261-279
    • Sirotkin, K.1
  • 6
    • 34248567845 scopus 로고    scopus 로고
    • Iterative saturation mutagenesis (ISM) for rapid directed evolution of functional enzymes
    • Reetz,M.T. and Carballeira,J.D. (2007) Iterative saturation mutagenesis (ISM) for rapid directed evolution of functional enzymes. Nat. Protoc., 2, 891-903.
    • (2007) Nat. Protoc , vol.2 , pp. 891-903
    • Reetz, M.T.1    Carballeira, J.D.2
  • 7
    • 0033280672 scopus 로고    scopus 로고
    • Exploring nonnatural evolutionary pathways by saturation mutagenesis: Rapid improvement of protein function
    • Miyazaki,K. and Arnold,F.H. (1999) Exploring nonnatural evolutionary pathways by saturation mutagenesis: Rapid improvement of protein function. J. Mol. Evol., 49, 716-720.
    • (1999) J. Mol. Evol , vol.49 , pp. 716-720
    • Miyazaki, K.1    Arnold, F.H.2
  • 8
    • 0032532156 scopus 로고    scopus 로고
    • Structural basis of spectral shifts in the yellow-emission variants of green fluorescent protein
    • Wachter,R.M., Elsliger,M.A., Kallio,K., Hanson,G.T. and Remington,S.J. (1998) Structural basis of spectral shifts in the yellow-emission variants of green fluorescent protein. Structure, 6, 1267-1277.
    • (1998) Structure , vol.6 , pp. 1267-1277
    • Wachter, R.M.1    Elsliger, M.A.2    Kallio, K.3    Hanson, G.T.4    Remington, S.J.5
  • 10
    • 0028559783 scopus 로고
    • Trinucleotide phosphoramidites: Ideal reagents for the synthesis of mixed oligonucleotides for random mutagenesis
    • Virnekas,B., Ge,L., Pluckthun,A., Schneider,K.C., Wellnhofer,G. and Moroney,S.E. (1994) Trinucleotide phosphoramidites: Ideal reagents for the synthesis of mixed oligonucleotides for random mutagenesis. Nucleic Acids Res., 22, 5600-5607.
    • (1994) Nucleic Acids Res , vol.22 , pp. 5600-5607
    • Virnekas, B.1    Ge, L.2    Pluckthun, A.3    Schneider, K.C.4    Wellnhofer, G.5    Moroney, S.E.6
  • 11
    • 0029810446 scopus 로고    scopus 로고
    • A convenient approach to the synthesis of trinucleotide phosphoramidites-synthons for the generation of oligonucleotide/peptide libraries
    • Kayushin,A.L., Korosteleva,M.D., Miroshnikov,A.I., Kosch,W., Zubov,D. and Piel,N. (1996) A convenient approach to the synthesis of trinucleotide phosphoramidites-synthons for the generation of oligonucleotide/peptide libraries. Nucleic Acids Res., 24, 3748-3755.
    • (1996) Nucleic Acids Res , vol.24 , pp. 3748-3755
    • Kayushin, A.L.1    Korosteleva, M.D.2    Miroshnikov, A.I.3    Kosch, W.4    Zubov, D.5    Piel, N.6
  • 12
    • 0028865576 scopus 로고
    • The synthesis of blocked triplet-phosphoramidites and their use in mutagenesis
    • Ono,A., Matsuda,A., Zhao,J. and Santi,D.V. (1995) The synthesis of blocked triplet-phosphoramidites and their use in mutagenesis. Nucleic Acids Res., 23, 4677-4682.
    • (1995) Nucleic Acids Res , vol.23 , pp. 4677-4682
    • Ono, A.1    Matsuda, A.2    Zhao, J.3    Santi, D.V.4
  • 13
    • 0028978348 scopus 로고
    • Mutagenesis using trinucleotide beta-cyanoethyl phosphoramidites
    • Lyttle,M.H., Napolitano,E.W., Calio,B.L. and Kauvar,L.M. (1995) Mutagenesis using trinucleotide beta-cyanoethyl phosphoramidites. Biotechniques, 19, 274-281.
    • (1995) Biotechniques , vol.19 , pp. 274-281
    • Lyttle, M.H.1    Napolitano, E.W.2    Calio, B.L.3    Kauvar, L.M.4
  • 14
    • 37748999981 scopus 로고    scopus 로고
    • Trinucleotide cassettes increase diversity of T7 phage-displayed peptide library
    • Krumpe,L.R., Schumacher,K.M., McMahon,J.B., Makowski,L. and Mori,T. (2007) Trinucleotide cassettes increase diversity of T7 phage-displayed peptide library. BMC Biotechnol., 7, 65.
    • (2007) BMC Biotechnol , vol.7 , pp. 65
    • Krumpe, L.R.1    Schumacher, K.M.2    McMahon, J.B.3    Makowski, L.4    Mori, T.5
  • 15
    • 0032030286 scopus 로고    scopus 로고
    • Codon-based mutagenesis using dimer-phosphoramidites
    • Neuner,P., Cortese,R. and Monaci,P. (1998) Codon-based mutagenesis using dimer-phosphoramidites. Nucleic Acids Res., 26, 1223-1227.
    • (1998) Nucleic Acids Res , vol.26 , pp. 1223-1227
    • Neuner, P.1    Cortese, R.2    Monaci, P.3
  • 17
    • 0035260476 scopus 로고    scopus 로고
    • Orthogonal combinatorial mutagenesis: A codon-level combinatorial mutagenesis method useful for low multiplicity and amino acid-scanning protocols
    • Gaytan,P., Yanez,J., Sanchez,F. and Soberon,X. (2001) Orthogonal combinatorial mutagenesis: A codon-level combinatorial mutagenesis method useful for low multiplicity and amino acid-scanning protocols. Nucleic Acids Res., 29, E9.
    • (2001) Nucleic Acids Res , vol.29
    • Gaytan, P.1    Yanez, J.2    Sanchez, F.3    Soberon, X.4
  • 18
    • 19244376986 scopus 로고    scopus 로고
    • Novel ceftazidime-resistance beta-lactamases generated by a codon-based mutagenesis method and selection
    • Gaytan,P., Osuna,J. and Soberon,X. (2002) Novel ceftazidime-resistance beta-lactamases generated by a codon-based mutagenesis method and selection. Nucleic Acids Res., 30, e84.
    • (2002) Nucleic Acids Res , vol.30
    • Gaytan, P.1    Osuna, J.2    Soberon, X.3
  • 19
    • 0024520745 scopus 로고
    • Site-directed mutagenesis by overlap extension using the polymerase chain reaction
    • Ho,S.N., Hunt,H.D., Horton,R.M., Pullen,J.K. and Pease,L.R. (1989) Site-directed mutagenesis by overlap extension using the polymerase chain reaction. Gene, 77, 51-59.
    • (1989) Gene , vol.77 , pp. 51-59
    • Ho, S.N.1    Hunt, H.D.2    Horton, R.M.3    Pullen, J.K.4    Pease, L.R.5
  • 20
    • 33947441649 scopus 로고
    • The kinetics of diffusion controlled molecular and ionic reactions in solution as determined by measurements of the quenching of fluorescence
    • Umberger,J.Q. and LaMer,V.K. (1945) The kinetics of diffusion controlled molecular and ionic reactions in solution as determined by measurements of the quenching of fluorescence. J. Am. Chem. Soc., 67, 1099-1109.
    • (1945) J. Am. Chem. Soc , vol.67 , pp. 1099-1109
    • Umberger, J.Q.1    LaMer, V.K.2
  • 21
    • 1842586731 scopus 로고    scopus 로고
    • Efficient and flexible access to fully protected trinucleotides suitable for DNA synthesis by automated phosphoramidite chemistry
    • Zehl,A., Starke,A., Cech,D., Hartsch,T., Merkl,R. and Fritz,H. (1996) Efficient and flexible access to fully protected trinucleotides suitable for DNA synthesis by automated phosphoramidite chemistry. Chem. Commun., 2677-2678.
    • (1996) Chem. Commun , pp. 2677-2678
    • Zehl, A.1    Starke, A.2    Cech, D.3    Hartsch, T.4    Merkl, R.5    Fritz, H.6
  • 24
    • 0029973636 scopus 로고    scopus 로고
    • FACS-optimized mutants of the green fluorescent protein (GFP)
    • Cormack,B.P., Valdivia,R.H. and Falkow,S. (1996) FACS-optimized mutants of the green fluorescent protein (GFP). Gene, 173, 33-38.
    • (1996) Gene , vol.173 , pp. 33-38
    • Cormack, B.P.1    Valdivia, R.H.2    Falkow, S.3
  • 25
    • 0032905565 scopus 로고    scopus 로고
    • Spectral variants of green fluorescent protein
    • Palm,G.J. and Wlodawer,A. (1999) Spectral variants of green fluorescent protein. Methods Enzymol., 302, 378-394.
    • (1999) Methods Enzymol , vol.302 , pp. 378-394
    • Palm, G.J.1    Wlodawer, A.2
  • 26
    • 34447304863 scopus 로고    scopus 로고
    • Flores-Ramirez,G., Rivera,M., Morales-Pablos,A., Osuna,J., Soberon,X. and Gaytan,P. (2007) The effect of amino acid deletions and substitutions in the longest loop of GFP. BMC Chem. Biol., 7 1.
    • Flores-Ramirez,G., Rivera,M., Morales-Pablos,A., Osuna,J., Soberon,X. and Gaytan,P. (2007) The effect of amino acid deletions and substitutions in the longest loop of GFP. BMC Chem. Biol., 7 1.
  • 27
    • 0347364647 scopus 로고    scopus 로고
    • Generating a synthetic genome by whole genome assembly: PhiX174 bacteriophage from synthetic oligonucleotides
    • Smith,H.O., Hutchison,C.A. III, Pfannkoch,C. and Venter,J.C. (2003) Generating a synthetic genome by whole genome assembly: PhiX174 bacteriophage from synthetic oligonucleotides. Proc. Natl Acad. Sci. USA, 100, 15440-15445.
    • (2003) Proc. Natl Acad. Sci. USA , vol.100 , pp. 15440-15445
    • Smith, H.O.1    Hutchison III, C.A.2    Pfannkoch, C.3    Venter, J.C.4
  • 28
    • 0031663369 scopus 로고    scopus 로고
    • The green fluorescent protein
    • Tsien,R.Y. (1998) The green fluorescent protein. Annu. Rev. Biochem. 67, 509-544.
    • (1998) Annu. Rev. Biochem , vol.67 , pp. 509-544
    • Tsien, R.Y.1


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.