Recombinant interferon-γ secreted by Chinese hamster ovary-320 cells cultivated in suspension in protein-free media is protected against extracellular proteolysis by the expression of natural protease inhibitors and by the addition of plant protein hydrolysates to the culture medium

In Vitro Cellular and Developmental Biology - Animal

Volumn 41, Issue 3-4, 2005, Pages 83-91

  Mols, J ^a   Peeters Joris, C ^a   Wattiez, R ^b   Agathos, S N ^a   Schneider, Y J ^a  

^a UNIVERSITÉ CATHOLIQUE DE LOUVAIN   (Belgium)

^b UNIVERSITY OF MONS   (Belgium)

Author keywords

Chinese hamster ovary cells; Ectopeptidases; Matrix metalloproteinases; Plant peptones; Plasminogen activators

Indexed keywords

DIPEPTIDYL PEPTIDASE IV; GELATINASE A; GELATINASE B; INTERSTITIAL COLLAGENASE; MATRIX METALLOPROTEINASE; MATRIX METALLOPROTEINASE 14; PEPTIDASE; PEPTONE; PLASMINOGEN ACTIVATOR INHIBITOR 1; PROTEIN PRECURSOR; PROTEINASE INHIBITOR; RECOMBINANT GAMMA INTERFERON; RECOMBINANT PROTEIN; TISSUE INHIBITOR OF METALLOPROTEINASE 1; TISSUE INHIBITOR OF METALLOPROTEINASE 2; TISSUE PLASMINOGEN ACTIVATOR; TRIPEPTIDYL PEPTIDASE II; UNCLASSIFIED DRUG; UROKINASE;

AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; CELL CULTURE; CHO CELL; CULTURE MEDIUM; GENE EXPRESSION; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMINOGEN ACTIVATION; POLYMERASE CHAIN REACTION; PROTEIN DEGRADATION; PROTEIN SECRETION;

ANIMALS; CHO CELLS; CRICETINAE; CULTURE MEDIA; HYDROLYSIS; INTERFERON-GAMMA, RECOMBINANT; KINETICS; PEPTIDE HYDROLASES; PLANT PROTEINS; PROTEASE INHIBITORS;

ANIMALIA; CRICETULUS GRISEUS; MAMMALIA;

EID: 22344454469     PISSN: 10712690     EISSN: None     Source Type: Journal    
DOI: 10.1290/0411075.1     Document Type: Article

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