Expression of recombinant antibacterial lactoferricin-related peptides from Pichia pastoris expression system

Journal of Agricultural and Food Chemistry

Volumn 57, Issue 20, 2009, Pages 9509-9515

  Chen, Gen Hung ^a   Chen, Wei Ming ^b   Huang, Guo Ting ^a   Chen, Yu Wen ^a   Jiang, Shann Tzong ^a,b  

^a PROVIDENCE UNIVERSITY   (Taiwan)

^b NATIONAL TAIWAN OCEAN UNIVERSITY   (Taiwan)

Author keywords

Antimicrobial peptides; Goat lactoferricin; Pichia pastoris

Indexed keywords

ANTIMICROBIAL CATIONIC PEPTIDE; HYBRID PROTEIN; LACTOFERRICIN B; LACTOFERRIN;

ANIMAL; ARTICLE; BACTERIUM; DRUG EFFECT; GENE EXPRESSION; GENETICS; GOAT; ISOLATION AND PURIFICATION; METABOLISM; PICHIA; BACTERIA; DRUG EFFECTS;

ANIMALS; ANTIMICROBIAL CATIONIC PEPTIDES; BACTERIA; GENE EXPRESSION; GOATS; LACTOFERRIN; PICHIA; RECOMBINANT FUSION PROTEINS;

BACILLUS CEREUS; BACTERIA (MICROORGANISMS); CAPRA HIRCUS; ESCHERICHIA; LISTERA; PICHIA PASTORIS; PSEUDOMONAS AERUGINOSA; STAPHYLOCOCCUS AUREUS;

EID: 70350326023     PISSN: 00218561     EISSN: None     Source Type: Journal    
DOI: 10.1021/jf902611h     Document Type: Article

References (50)

1. Khardori, N. Antibiotics-past, present, and future. Med. Clin. North. Am. 2006, 90, 1049-1076.
2. Huey, W. H. Action of antimicrobial peptides: two-state model. Biochemistry 2000, 39, 8347-8352
3. Kamysz, W.; Okroj, M.; Lukasiak, J. Novel properties of antimicrobial peptides. Acta Biochim. Pol. 2002, 50, 461-469.
4. Otvos, L. Jr. Antibacterial peptides and proteins with multiple cellular targets. J. Pept. Sci. 2005, 11, 697-706.
5. (5) Toke, O. Antimicrobial peptides: new candidates in the fight against bacterial infections. Biopolymers 2005, 80, 717-735. (Pubitemid 43266580)
6. Dubin, A.; Mak, P.; Dubin, G.; Rzychon, M.; Stec-Niemczyk, J.; Wladyka, B.; Maziarka, K.; Climiel, D. New generation of peptide antibiotics. Acta. Biochim. Pol. 2005, 52, 633-638.
7. Gentilucci, L.; Tolomelli, A.; Squassabia, F. Peptides and peptidomimetics in medicine, surgery and biotechnology. Curr. Med. Chem. 2006, 13, 2449-2466.
8. Jenssen, H.; Hamill, P.; Hancock, R. E. Peptide antimicrobial agents. Clin. Microbiol. Rev. 2006, 19, 491-511.
9. Marr, A. K.; Gooderham, W. J.; Hancock, R. E. Antibacterial peptides for therapeutic use: obstacles and realistic outlook. Curr. Opin. Pharmacol. 2006 6 468-472.
10. Andreu, D.; Rivas, L. Animal antimicrobial peptides: an overview. Biopolymers 1998, 47, 415-433.
11. Hancock, R. E. W.; Diamond, G. The role of cationic antimicrobial peptides in innate host defences. Trends Microbiol 2000, 8, 402-410.
12. Andres, E.; Dimarcq, J. L. Cationic antimicrobial, peptides: from innate immunity study to drug development. Med. Mol. Infect. 2007, 37, 194-199.
13. Ooi, C. E.; Weiss, J.; Doerfler, M. E.; Elsbach, P. Endotoxinneutralizing properties of the 25 kDa N-terminal fragment and a newly isolated 30 kDa C-terminal fragment of the 55-60 kDa bactericidal/permeability-increasing protein of human neutrophils. J. Exp. Med. 1991, 174, 649-655.
14. Lee, K. H.; Hong, S. Y.; Oh, J. E.; Kwon, M. Y.; Yoon, J. H.; Lee, J. H.; Lee, B. L.; Moon, H. M. Identification and characterization of the antimicrobial peptide corresponding to C-terminal β-sheet domain of tenecin 1, an antibacterial protein of larvae of Tenebrio molitor. Biochem. J. 1998, 334, 99-105.
15. Fernandes, J. M.; Saint, N.; Kemp, G. D.; Smith, V. J. Oncorhyncin III: a potent antimicrobial peptide derived from the non-histonechromosomal protein H6 of rainbow trout, Oncorhynchus mykiss. Biochem. J. 2003, 373, 621-628.
16. (16) Oren, Z.; Shai, Y. Mode of action of linear amphipathic a-helical antimicrobial peptides. Biopolymers 1998, 47, 451-463. (Pubitemid 128637353)
17. Shai, Y. Mode of action of membrane active antimicrobial peptides. Biopolymers 2002, 66, 236-248.
18. (18) Strom, M. B.; Rekdal, O.; Svendsen, J. S. Antibacterial activity of 15-residue lactoferricin derivatives. J. Pept. Res. 2000, 56, 265-274. (Pubitemid 30800256)
19. (19) Haug, B. E.; Svendsen, J. S. The role of tryptophan in the antibacterial activity of a 15-residue bovine lactoferricin peptide. J. Pept. Sci. 2001, 7, 190-196. (Pubitemid 32422063)
20. (20) Strom, M. B.; Rekdal, O.; Svendsen, J. S. Antimicrobial activity of short arginine- and tryptophan-rich peptides. J. Pept. Sci. 2002, 8, 431-437. (Pubitemid 34893655)
21. (21) Orsi, N. The antimicrobial activity of lactoferrin: current status and perspectives. Biometals 2004, 17, 189-196. (Pubitemid 38987136)
22. Gifford, J. L.; Hunter, H. N.; Vogel, H. J. Lactoferricin: a lactoferrin-derived peptide with antimicrobial, antiviral, antitumor and immunological properties. Cell Mol. Life Sci. 2005, 62, 2588-2598.
23. Ling, J. M.; Schryvers, A. B. Perspectives on interactions between lactoferrin and bacteria. Biochem. Cell Biol. 2006, 84, 275-281.
24. Munoz, A.; Marcos, J. F. Activity and mode of action against fungal phytopathogens of bovine lactoferricin-derived peptides. J. Appl. Microbiol. 2006, 101, 1199-1207.
25. Bellamy, W.; Takase, M.; Wakabayashi, H.; Kawase, K.; Tomita, M. Antibacterial spectrum of lactoferricin B, a potent bactericidal peptide derived from the N-terminal region of bovine lactoferrin. J. Appl. Bacteriol. 1992, 73 472-479.
26. Bellamy, W.; Takase, M.; Yamauchi, K.; Wakabayashi, H.; Kawase, K.; Tomita, M. Identification of the bactericidal domain of lactoferrin. Biochim. Biophys. Acta 1992, 1121, 130-136.
27. Vogel, H. J.; Schibli, D. J.; Jing, W.; Lohmeier-Vogel, E. M.; Epand, R. F.; Epand, R. M. Towards a structure-function analysis of bovine lactoferricin and related tryptophan- and arginine-containing peptides. Biochem. Cell Biol. 2002, 80, 49-63.
28. Jenssen, H. Anti herpes simplex virus activity of lactoferrin/ lactoferricin-an example of antiviral activity of antimicrobial, protein/ peptide. Cell. Mol. Life Sci. 2005, 62, 3002-3013.
29. (29) Mader, J. S.; Salsman, J.; Conrad, D. M.; Hoskin, D. W. Bovine lactoferricin selectively induces apoptosis in human leukemia and carcinoma cell lines. Mol. Cancer Ther. 2005, 4, 612-624 (Pubitemid 40601843)
30. Centeno, J. M.; Burguete, M. C.; Castello-Ruiz, M.; Enrique, M.; Valles, S.; Salom, J. B.; Torregrosa, G.; Marcos, J. F.; Alborch, E.; Manzanares, P. Lactoferricin-related peptides with inhibitory effects on ACE-dependent vasoconstriction. J. Agric. Food Chem. 2006, 54, 5323-5329.
31. Sambrook, J.; Fritsch, E. F.; Maniatis, T. Plasmid vector. In Molecular cloning: a laboratory mamuti, 2nd ed.; Sambrook, J., Fritsch, E. F., Maniatis, T., Eds.; Cold Spring Harbor Laboratory Press: New York, 1989; pp 72-73.
32. Paramasivam, M.; Saravanan, K.; Uma, K.; Sharma, S.; Singh, T. P.; Srinivasan, A. Expression, purification, and characterization of equine lactoferrin in Pichia pastoris. Protein Expression Purif. 2002, 26, 28-34.
33. (33) Holm, C.; Meeks-wagner, D. W.; Fangman, W. L.; Botstein, D. A rapid, efficient method for isolating DNA from yeast. Gene 1986, 42, 169-173 (Pubitemid 16090809)
34. Kapoor, K. N.; Barry, D. T.; Rees, R. C.; Dodi, I. A.; McArdle, S. E. B.; Creaser, C. S.; Bonner, P. L. R. Estimation of peptide concentration by a modified bicinchoninic acid assay. Anal. Bioehem. 2009, 595, 138-140.
35. Edman, P. Sequence determination (Review). Mol. Biol. Biochem. Biophys. 1970, 8, 211-255.
36. Piddock, L. J. V. Techniques used for the determination of antimicrobial resistance and sensitivity in bacteria. J. Appl. Bacterol. 1990, 68, 307-318.
37. Daly, R.; Hearn, M. T. W. Review Expression of heterologous proteins in Pichia pastoris: a useful experimental tool in protein engineering and production. J. Mol. Recognit. 2005, 18, 119-138.
38. Chen, G. H.; Yin, L. J.; Chiang, I. H.; Jiang, S. T. Cloning and Expression of Antibacterial Goat Lactoferricin from Escherichia coli AD494(DE3)pLysS Expression System. J. Food Prot. 2008, 71, 2523-2525.
39. Feng, X. J.; Wang, J. H.; Shan, A. S.; Teng, D.; Yang, Y. L.; Yao, Y.; Yang, G. P.; Shao, Y. C.; Liu, S.; Zhang, F. Fusion expression of bovine lactoferricin in Escherichia coli, Protein Expression Purif. 2006, 47, 110-117.
40. Xu, X.; Jin, F.; Yu, X.; Ji, S.; Wang, J.; Cheng, H.; Wang, C.; Zhang, W. Expression and puriWcation of a recombinant antibacterial peptide, cecropin, from Escherichia coli. Protein Expression Purif. 2007, 53, 293-301.
41. Xu, X.; Jin, F.; Yu, X.; Ren, S.; Hu, J.; Zhang, W. High-level expression of the recombinant hybrid peptide cecropinA(1-8)-magainin2(1-12) with an ubiquitin fusion partner in Escherichia coli. Protein Expression Purif. 2007, 55, 175-182.
42. Guerreiro, C. I.P.D.; Fontes, C. M.G.A.; Gama, M.; Domingues, L. Escherichia coli expression and purification of four antimicrobial peptides fused to a family 3 carbohydrate-binding module (CBM) from Clostridium thermocellum. Protein Expression Purif. 2008, 59, 161-168.
43. Park, T. J.; Kim, J. S.; Choi, S. S.; Kim, Y. Cloning, expression, isotope labeling, purification, and characterization of bovine antimicrobial peptide, lactophoricin in Escherichia coll Protein Exnression Purif. 2009, 65, 23-29.
44. Zorko, M.; Japelj, B.; Hafner-Bratkovič, I.; Jerala, R. Expression, purification and structural studies of a short antimicrobial peptide. Biochim. Biophys. Acta. Biomembr. 2009, 1788, 314-323.
45. Zhang, J.; Zhang, S. Q.; Wu, X.; Chen, Y. Q.; Diao, Z. Y. Expression and characterization of antimicrobial peptide ABP-CM4 in methylotrophic yeast Pichia pastoris, Process Biochem. 2006, 41, 251-256.
46. Jin, F. L.; Xu, X. X.; Yu, X. Q.; Ren, S. X. Expression and characterization of antimicrobial peptide CecropinAD in the methylotrophic yeast Pichia pastoris. Process Biochem. 2009, 44, 11-16.
47. Yamada, K.; Nakajima, Y.; Natori, S. Production of reombinant sarcotoxin IA in Bombyx mori cells. Biochem. J. 1990, 272, 633-636.
48. Fiordalisi, J. J.; Fetter, C. H.; TenHarmsel, A.; Gigowski, R.; Chiappinelli, V. A.; Grant, G. A. Synthesis and expression in Escherichia coli of a gene for κ-bungarotoxin. Biochemistry 1991, 30, 10337-10343.
49. Latham, P. W. Therapeutic peptides revisited. Nat. Biotechnol. 1999, 77, 755-777.
50. Kang, J. H.; Lee, M. K.; Kim, K. L.; Hahm, K. S. Structurebiological activity relationships of 11-residue highly basic peptide segment of bovine lactoferrin. Int. J. Pent. Protein Res. 1996, 48, 357-363.