Estimation of peptide concentration by a modified bicinchoninic acid assay

Analytical Biochemistry

Volumn 393, Issue 1, 2009, Pages 138-140

  Kapoor, Krishan N ^a   Barry, Danielle T ^a   Rees, Robert C ^a   Anthony Dodi, I ^a   McArdle, Stephanie E B ^a   Creaser, Colin S ^b   Bonner, Philip L R ^a  

^a NOTTINGHAM TRENT UNIVERSITY   (United Kingdom)

^b LOUGHBOROUGH UNIVERSITY   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

SODIUM HYDROXIDE;

BICINCHONINIC ACIDS; ECONOMICAL ESTIMATION; HEAT DENATURATION; PROTEIN ASSAYS;

PEPTIDES;

BICINCHONINIC ACID; QUINOLINE DERIVATIVE; SODIUM HYDROXIDE; UNCLASSIFIED DRUG;

ACCURACY; ANIMAL TISSUE; ARTICLE; CHEMOREACTIVITY; CONTROLLED STUDY; HYDROPHOBICITY; INCUBATION TIME; NONHUMAN; PRIORITY JOURNAL; PROTEIN DENATURATION; PROTEIN DETERMINATION;

EID: 67650991809     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2009.06.016     Document Type: Article

References (10)

1. Stoscheck C.M. Quantitation of protein. Methods Enzymol. 182 (1990) 50-69
2. Schiltz E., Schnackerz D.K., and Gracy R.W. Comparison of ninhydrin fluorescamine, o-phthaldialdehyde for the detection of amino acids, peptides, their effects on the recovery, composition of peptides from thin-layer fingerprints. Anal Biochem. 79 (1977) 33-41
3. You W.W., Haughland R.P., Ryan D.K., and Haughland R.P. 3-(4-Carboxybenzoyl)quinoline-2-carboxaldehyde, a reagent with broad dynamic range for the assay of proteins and lipoproteins in solution. Anal Biochem. 244 (1997) 277-282
4. Coghlan D.R., Mackintosh J.A., and Karuso P. Mechanism of reversible fluorescent staining of protein with epicocconone. Org. Lett. 7 (2005) 2401-2404
5. Lowry D., Rosebrough N.J., Farr A.R., and Randall R.J. Protein measurement with Folin phenol reagent. J. Biol. Chem. 193 (1951) 265-275
6. Smith P.K., Krohn R.I., Hermanson G.T., Mallia A.K., Gartner F.H., Provenzano M.D., Fujimoto E.K., Goeke N.M., Olson R.J., and Klenk D.C. Measurement of protein using bicinchoninic acid. Anal. Biochem. 150 (1985) 76-85
7. Hua Y., and Scheller R.H. Three SNARE complexes cooperate to mediate membrane fusion. Proc. Natl. Acad. Sci. USA 98 (2001) 8065-8070
8. Faber C., Stallmann H.P., Lyaruu D.M., de Blieck J.M.A., Bervoets Th.J.M., van Nieuw Amerongen A., and Wuisman P.I.J.M. Release of antimicrobial peptide Dhvar-5 from polymethylmethacrylate beads. J. Antimicrob. Chemother. 51 (2003) 1359-1364
9. Brown R.E., Jarvis K.L., and Hyland K.J. Protein measurement using bicinchoninic acid: elimination of interfering substances. Anal Biochem. 180 (1989) 136-139
10. Akins R.E., and Tuan R.S. Measurement of protein in 20 s using a microwave BCA assay. Biotechniques 12 (1992) 496-499