Expression and characterization of antimicrobial peptide ABP-CM4 in methylotrophic yeast Pichia pastoris

Process Biochemistry

Volumn 41, Issue 2, 2006, Pages 251-256

  Zhang, Jie ^a   Zhang, Shuang Quan ^a   Wu, Xi ^a   Chen, Yu Qing ^a   Diao, Zhen Yu ^a  

^a NANJING NORMAL UNIVERSITY   (China)

Author keywords

ABP CM4; Antibacterial peptide; Antifungal; P. pastoris; pPICZaA; Recursive PCR

Indexed keywords

BIOASSAY; CHARACTERIZATION; ELECTROPHORESIS; GELS; PROTEINS; YEAST;

ABP-CM4; ANTIBACTERIAL PEPTIDE; ANTIFUNGAL; P. PASTORIS; PPICZAA; RECURSIVE PCR;

AMINO ACIDS;

PICHIA PASTORIS;

EID: 29244479264     PISSN: 13595113     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.procbio.2005.06.030     Document Type: Article

References (24)

1. H.G. Boman Antibacterial peptides: basic facts and emerging concepts J Intern Med 254 2003 197 215
2. T. Yizeng, S. Zhang, and Qu. Xianming Separation, purification of antibacterial CM4 and the research of the structure and character Sci China B 32 1989 473 480
3. S.q. Zhang, H.w. Jia, and D. Zhuying Ultrastructure observation of K562 leukemia cells treated with antibacterial peptide CM4 component Prog Biochem Biophys 24 1997 159 163
4. J. Xu, and S.q. Zhang The research of the mechanism of antibacterial peptide CM4 component against A. parasiticus Prog Nat Sci 11 2001 1263 1267
5. J. Xu, and S.q. Zhang The mechanism of antibacterial of antibacterial peptide CM4 component was observed by confocal laser scanning microscope Prog Nat Sci 10 2001 1105 1109
6. R.E. Hancock, and R. Lehrer Cationic peptides: a new source of antibiotics Trends Biotechnol 16 1998 82 88
7. R.E. Hancock, and A. Patrzykat Clinical development of cationic antimicrobial peptides: from natural to novel antibiotics Curr Drug Targets Infect Disord 2 2002 79 83
8. G. Diamond Natures antibiotics: the potential of antimicrobial peptides as new drugs Biologist (London) 48 2001 209 212
9. E. Andres, and J.L. Dimarcq Cationic anti-microbial peptides: from innate immunity study to drug development Rev Med Intern 25 2004 629 635
10. X. Li, Z.y. Dai, S.q. Zhang, and L.y. Zhang Studies on the changes of CM4 gene structure and the expression products in E. coli Acta Biochim Biophys Sinica 15 1999 387 391
11. J.L. Cereghino, and J.M. Cregg Heterologous protein expression in the methylotrophic yeast Pichia pastoris FEMS Microbiol Rev 24 2000 45 66
12. K. Sreekrishna, R.G. Brankamp, and K.E. Kropp Strategies for optimal synthesis and secretion of heterologous proteins in the methylotrophic yeast Pichia pastoris Gene 1990 1997 55 62
13. J.M. Cregg, J.L. Cereghino, J. Shi, and D.R. Higgins Recombinant protein expression in Pichia pastoris Mol Biotechnol 16 2000 23 52
14. H. Boze, L. Celine, C. Patrick, R. Fabien, V. Christine, C. Yves, and M. Guy High-level secretory production of recombinant porcine follicle-stimulating hormone by Pichia pastoris Process Biochem 36 2001 907 913
15. C.Y. Lee, S.J. Lee, K.H. Jung, S. Katoh, and E.K. Lee High dissolved oxygen tension enhances heterologous protein expression by recombinant Pichia pastoris Process Biochem 38 2003 1147 1154
16. C. Prodromou, and LH. Pearl Recursive PCR: a novel technique for total gene synthesis Protein Eng 5 1992 827 829
17. D. Hultmark, A. Engstrom, and H. Bennich Insect immunity: isolation and structure of cecropin d and four minor antibacterial components from cecropia pupae Eur J Biochem 127 1982 207 217
18. H. Schagger, and G. Von Jagow Tricine-sodium dodecyl sulfate polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa Anal Biochem 166 1987 368 379
19. D.G. Lee, Y.k. Park, H.N. Kim, H.K. KIM, P.Il. Kim, B.H. Choi, and K.S. Hahm Antifungal mechanism of an antimicrobial peptide, HP(2-20), derived from N-terminus of Helicobacter pylori ribosomal protein L1 against Candida albicans Biochem Biophys Res Commun 291 2002 1006 1013
20. M. Jahic, M. Gustavsson, A.-K. Jansen, M. Martinelle, and S.-O. Enfors Analysis and control of proteolysis of a fusion protein in Pichia pastoris fed-batch processes J Biotechnol 102 2003 45 53
21. X. Shi, T. Karkut, M. Chamankhah, M. Alting-Mees, S.M. Hemmingsen, and D. Hegedus Optimal conditions for the expression of a single-chain antibody (scFv) gene in Pichia pastoris Protein Express Purif 28 2003 321 330
22. Li S Z., F. Xiong, and Q. Lin Low-temperature increases the yield of biologically active herring antifreeze protein in Pichia pastoris Protein Express Purif 21 2001 438 445
23. M. Bencurova, D. Rendic, G. Fabini, E.M. Kopecky, F. Altmann, and I.B. Wilson Expression of eukaryotic glycosyltransferases in the yeast Pichia pastoris Biochimie 85 2003 413 422
24. J.J. Clare, M.A. Romanos, F.B. Rayment, J.E. Rowedder, M.A. Smith, M.M. Payne, K. Sreekrishna, and C.A. Henwood Production of mouse epidermal growth factor in yeast: high-level secretion using Pichia pastoris strains containing multiple gene copies Gene 105 1991 205 212