Construction of a Monascuspurpureas mutant showing lower citrinin and higher pigment production by replacement of ctnA with pks1 without using vector and resistance gene

Journal of Agricultural and Food Chemistry

Volumn 57, Issue 20, 2009, Pages 9764-9768

  Xu, Min Jun ^a,b   Yang, Ze Liang ^a   Liang, Zhi Zhou ^a   Zhou, Shi Ning ^a  

^a SUN YAT SEN UNIVERSITY   (China)

^b SOUTH CHINA AGRICULTURAL UNIVERSITY   (China)

Author keywords

Citrinin; Gene replacement; Monascus; Pigments; PksCT; Pksi 1

Indexed keywords

BACILLUS SUBTILIS; MONASCUS;

ANTIINFECTIVE AGENT; CITRININ; FUNGAL PROTEIN; PIGMENT;

ARTICLE; BIOSYNTHESIS; DRUG EFFECT; EVALUATION; GENE VECTOR; GENETIC ENGINEERING; GENETICS; METABOLISM; METHODOLOGY; MONASCUS; MUTATION;

ANTI-BACTERIAL AGENTS; CITRININ; FUNGAL PROTEINS; GENETIC ENGINEERING; GENETIC VECTORS; MONASCUS; MUTATION; PIGMENTS, BIOLOGICAL;

EID: 70350314815     PISSN: 00218561     EISSN: None     Source Type: Journal    
DOI: 10.1021/jf9023504     Document Type: Article

References (29)

1. Demain, A. L. Pharmaceutically active secondary metabolites of micro-organisms. Appl. Microbiol. Biotechnol. 1999, 52, 455-463.
2. Blanc, P. J.; Loret, M. O.; Santerre, A. L.; Pareilleux, A.; Prome, D.; Laussac, J. P.; Goma, G. Pigments of Monascus. J. Food Sci. 1994, 59, 862-865.
3. Lee, C. L.; Chen, W. P.; Wang, J. J.; Pan, T. M. A simple and rapid approach for removing citrinin while retaining monacolin K in red. mold rice. J. Agric. Food Chem. 2007, 55, 11101-11108.
4. Sweeney, M. J.; Dobson, A. D. Mycotoxin production by Aspergillus, Fusarium and Penicillium species. Int. J. Food Microbiol. 1998, 43, 141-158.
5. Blanc, P. J.; Laussac, J. P.; Le, B. J.; Le, B. P.; Loret, M. O.; Pareilleux, A.; Prome, D.; Prome, J. C.; Santerre, A. L.; Goma, G. Characterization of monascidin A from Monascus as citrinin. Int. J. Food. Microbiol. 1995, 27, 201-213.
6. Wang, J. J.; Lee, C. L.; Pan, T. M. Improvement of monacolin K, y-aminobutyric acid and citrinin production ratio as a function of environmental conditions of Monascus purpurem NTU 601. J. Ind. Microbiol. Biotechol. 2003, 30, 669-676.
7. Pattanagul, P.; Pinthong, R.; Phianmongkhol, A.; Tharatha, S. Mevinolin, citrinin and pigments of adlay angkak fermented by Monascus sp. Int. J. Food Microbiol. 2008, 126, 20-23.
8. Orozco, S. F.; Kilikian, B. V. Effect of pH on citrinin and red pigments production by Monascus purpureus CCT3802. World J. Microbiol. Biotechnol. 2008, 24, 263-268.
9. Babitha, S.; Carvahlo, J. C.; Soccol, C. R.; Pandey, A. Effect of light on growth, pigment production and culture morphology of Monasem purpureus in solid-state fermentation. World J. Microbiol. Biotechnol. 2008, 24, 2671-2675.
10. Shimizu, T.; Kinoshita, H.; Ishihara, S.; Sakai, K.; Nagai, S.; Nihira, T. Polyketide synthase gene responsible for citrinin biosynthesis in Monascus purpureus. Appl. Environ Microbiol. 2005, 71, 3453-3457.
11. (11) Shimizu, T.; Kinoshita, H.; Nihira, T. Development of transformation system in Monascus purpureus using an autonomous replication vector with, aureobasidin A. resistance gene. Biotechnol. Lett. 2006, 25, 115-120. (Pubitemid 41821482)
12. Shimizu, T.; Kinoshita, H.; Nihira, T. Identification and in vivo functional analysis by gene disruption of ctnR, an activator gene involved in citrinin biosynthesis in Monascus purpureus. Appl. Environ. Microbiol. 2007, 73, 5097-5103.
13. Sakai, K.; Kinoshita, H.; Shimizu, T.; Nihira, T. Construction of a citrinin gene cluster expression system in heterologous Aspergillus oryzae. J. Biosci. Bioeng. 2008, 106, 466-472.
14. Chen, Y. P.; Tseng, C. P.; Chien, I. L.; Wang, W. Y.; Liaw, L. L.; Yuan, G. F. Exploring the distribution of citrinin biosynthesis related genes among Monascus species. J. Agric. Food Chem. 2008, 56, 11767-11772.
15. Wang, Y. Z.; Ju, X. L.; Zhou, Y. G. The variability of citrinin production in Monascus type cultures. Food Microbiol. 2005, 22, 145-148.
16. Fu, G. M.; Xu, Y.; Li, Y. P.; Tan, W. H. Construction of a replacement vector to disrupt pksCT gene for the mycotoxin citrinin biosynthesis in Monascus aurantiacus and maintain food red pigment production. Asia Pac. J. Clin. Nutr. 2007, 16 (supplement 1), 137-142.
17. Takano, Y.; Kubo, Y.; Shimizu, K.; Mise, K.; Okuno, T.; Furusawa, I. Structural analysis of PKS1, a polyketide synthase gene involved, in melanin biosynthesis in Colletotrichum lagenarium. Mol. Gen. Genet. 2007, 249, 162-167.
18. Peter, L.; Philippe, T.; Colette, B. Isolation and disruption of the melanin pathway polyketide synthase gene of the softwood deep stain fungus Ceratocystis resinífera. Fungal Genet. Biol. 2004, 41, 33-41.
19. Walton, J. D. Horizontal gene transfer and the evolution of secondary metabolite gene clusters in fungi, a hypothesis. Funeal Genet. Biol. 2000, 30, 167-171.
20. Horton, R. M.; Hunt, H. D.; Ho, S. N.; Pullen, J. K.; Pease, L. R. Engineering hybrid genes without the use of restriction enzymes, gene splicing by overlap extension. Gene 1989, 77, 61-68.
21. Bölker, M.; Bohnert, H. U.; Braun, K. H.; Görl, J.; Kallmann, R. Tagging pathogenicity genes in Ustilago maydis by restriction enzyme-mediated integration (REMI). Mol. Gen. Genet. 1995, 248, 547-552.
22. Wang, J. J.; Lee, C. L.; Pan, T. M. Modified mutation method for screening low citrinin-producing strains of Monascus purpureus on rice culture. J. Agric. Food Chem. 2004, 52, 6977-16782
23. Yang, X. C.; Hu, W. L.; Xie, F. J.; Wang, M. J. Cirinin control in processing Monascus red. Chin. Food Addit. 2007, 1, 209-212.
24. Bingle, L. E.; Simpson, T. J.; Lazarus, C. M. Ketosynthase domain probes identify two subclasses of fungal polyketide synthase genes. Funeal Genet. Biol. 1999, 26, 209-223.
25. Chen, S. Y.; Cao, J. W. Fonnation and regeneration of protoplasts from several filamentous fungi. Mycosystema 1986, 5, 117-123.
26. Zhou, L. H.; Li, G. Q.; Wang, Z. X.; Zhu, G. J. Preparation and. regeneration of protoplasts from Monascus purpureus and genetic transformation system. Hereditas 2005, 27, 423-428.
27. 13C nuclear magnetic resonance. Appl, Environ, Microbol, 1999, 65, 311-314.
28. (28) Vrabcheva, T.; Usleber, E.; Dietrich, R.; Märtlbauer, E. Co-occurrence of ochratoxin A and citrinin in cereals from Bulgarian villages with a history of Balkan endemic nephropathy. J. Agric. Food Chem. 2000, 48, 2483-2488. (Pubitemid 30431262)
29. Li, F. Q.; Xu, G. R.; Li, Y. W.; Chen, Y.; Ji, R. Natural occurrence of citrinin in Monascus products. J. Hyg, Res. 2005, 34, 451-454.