Improving quantitative real-time RT-PCR reproducibility by boosting primer-linked amplification efficiency

Biotechnology Letters

Volumn 24, Issue 24, 2002, Pages 2053-2056

  Tichopad, Ales ^a   Dzidic, Anamarija ^a   Pfaffl, Michael W ^a  

^a TECHNICAL UNIVERSITY OF MUNICH   (Germany)

Author keywords

Gene quantification; PCR efficiency; Primers; RT PCR

Indexed keywords

SIGMOIDEA;

EID: 0036955404     PISSN: 01415492     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1021319421153     Document Type: Article

References (9)

1. Gibson UE, Heid CA, Williams PM (1996) A novel method for real time quantitative RT-PCR. Genome Res. 6: 1095-1101.
2. Liu W, Saint DA (2002) A new quantitative method of real time reverse transcription polymerase chain reaction assay based on simulation of polymerase chain reaction kinetics. Anal Biochem. 302: 52-59.
3. Meijerink J, Mandigers C, van de Locht L, Tonnissen E, Goodsaid F, Raemaekers J (2001) A novel method to compensate for different amplification efficiencies between patient DNA samples in quantitative real-time PCR. J. Mol. Diagn. 3: 55-61.
4. Peccoud J, Jacob C (1996) Theoretical uncertainty of measurements using polymerase chain reaction. Biophys. J. 71: 101-108.
5. Pfaffl MW (2001) A new mathematical model for relative quantification in real-time RT-PCR. Nucl. Acids Res. 29: 2002-2007.
6. Rasmussen R (2001) Quantification on the LightCycler instrument. In: Meuer S, Wittwer C, Nakagawara K, eds. Rapid Cycle Real-time PCR: Methods and Applications. Heidelberg: Springer-Verlag Press, pp. 21-34.
7. Ririe KM, Rasmussen RT, Wittwer CT (1997) Product differentiation by analysis of DNA melting curves during the polymerase chain reaction. Anal. Biochem. 245: 154-160.
8. Schmittgen TD (2001) Real-time quantitative PCR. Methods 25: 383-385.
9. Wittwer CT, Ririe KM, Andrew RV, David DA, Gundry RA, Balls UJ (1997) The LightCycler: a microvolume multisample fluorimeter with rapid temperature control. Biotechniques 22: 176-181.