An in vivo imaging-based assay for detecting protein interactions over a wide range of binding affinities

Analytical Biochemistry

Volumn 395, Issue 2, 2009, Pages 166-177

  Edwards, A Nicole ^a   Fowlkes, Jason D ^a   Owens, Elizabeth T ^a   Standaert, Robert F ^a   Pelletier, Dale A ^a   Hurst, Gregory B ^a   Doktycz, Mitchel J ^a   Morrell Falvey, Jennifer L ^a  

^a OAK RIDGE NATIONAL LABORATORY   (United States)

Author keywords

Binding affinity; DivIVA; E. coli; Live cell imaging; Protein interaction; Rhodopseudomonas palustris

Indexed keywords

BACTERIOLOGY; BINDING ENERGY; CELL PROLIFERATION; CLONE CELLS; ESCHERICHIA COLI; MASS SPECTROMETRY;

BINDING AFFINITIES; DIVIVA; E. COLI; LIVE-CELL IMAGING; PROTEIN INTERACTION; RHODOPSEUDOMONAS PALUSTRIS;

PROTEINS;

GREEN FLUORESCENT PROTEIN; KARYOPHERIN ALPHA;

AMINO TERMINAL SEQUENCE; ARTICLE; BACILLUS SUBTILIS; BINDING AFFINITY; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; IMAGE ANALYSIS; IN VIVO STUDY; MASS SPECTROMETRY; MOLECULAR CLONING; NONHUMAN; NUCLEAR LOCALIZATION SIGNAL; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN INTERACTION; PROTEIN LOCALIZATION; RHODOPSEUDOMONAS PALUSTRIS; SENSITIVITY ANALYSIS;

BACILLUS SUBTILIS; EUKARYOTA; RHODOPSEUDOMONAS PALUSTRIS;

EID: 70349782303     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2009.08.015     Document Type: Article

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