A novel cytology-based, two-hybrid screen for bacteria applied to protein-protein interaction studies of a Type IV secretion system

Journal of Bacteriology

Volumn 184, Issue 20, 2002, Pages 5572-5582

  Ding, Zhiyong ^a   Zhao, Zhenming ^a   Jakubowski, Simon J ^a   Krishnamohan, Atmakuri ^a   Margolin, William ^a   Christie, Peter J ^a  

^a UNIVERSITY OF TEXAS MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; GREEN FLUORESCENT PROTEIN; LEUCINE ZIPPER PROTEIN;

ARTICLE; BACILLUS SUBTILIS; CELL SECRETION; CYTOLOGY; ESCHERICHIA COLI; MOLECULAR DYNAMICS; NONHUMAN; PRIORITY JOURNAL; PROTEIN LOCALIZATION; PROTEIN PROTEIN INTERACTION; PROTEIN TARGETING; RHIZOBIUM RADIOBACTER; TWO HYBRID SYSTEM;

BACILLUS SUBTILIS; BACTERIAL PROTEINS; CELL CYCLE PROTEINS; CELL DIVISION; CYTOSKELETAL PROTEINS; DNA, BACTERIAL; DNA-BINDING PROTEINS; ESCHERICHIA COLI; LEUCINE ZIPPERS; MEMBRANE PROTEINS; PROTEIN BINDING; PROTEIN KINASES; RECOMBINANT FUSION PROTEINS; RHIZOBIUM RADIOBACTER; SACCHAROMYCES CEREVISIAE PROTEINS; TWO-HYBRID SYSTEM TECHNIQUES;

AGROBACTERIUM; AGROBACTERIUM TUMEFACIENS; BACILLUS SUBTILIS; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; HOMO; NEGIBACTERIA; POSIBACTERIA;

EID: 0036785309     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.184.20.5572-5582.2002     Document Type: Article

References (55)

1. Addinall, S. G., and J. Lutkenhaus. 1996. FtsZ-spirals and -arcs determine the shape of the invaginating septa in some mutants of Escherichia coli. Mol. Microbiol. 22:231-237.
2. Bagdasarian, M., R. Lurz, B. Ruckert, F. C. Franklin, M. M. Bagdasarian, J. Frey, and K. N. Timmis. 1981. Specific-purpose plasmid cloning vectors. II. Broad host range, high copy number, RSF1010-derived vectors, and a host-vector system for gene cloning in Pseudomonas. Gene 16:237-247.
3. Banta, L. M., J. Bohne, S. D. Lovejoy, and K. Dostal. 1998. Stability of the Agrobacterium tumefaciens VirB10 protein is modulated by growth temperature and periplasmic osmoadaption. J. Bacteriol. 180:6597-6606.
4. Baron, C., N. Domke, M. Beinhofer, and S. Hapfelmeier. 2001. Elevated temperature differentially affects virulence, VirB protein accumulation, and T-pilus formation in different Agrobacterium tumefaciens and Agrobacterium vitis strains. J. Bacteriol. 183:6852-6861.
5. Beaupré, C. E., J. Bohne, E. M. Dale, and A. N. Binns. 1997. Interactions between VirB9 and VirB10 membrane proteins involved in movement of DNA from Agrobacterium tumefaciens into plant cells. J. Bacteriol. 179:78-89.
6. Beech, P. L., T. Nheu, T. Schultz, S. Herbert, T. Lithgow, P. R. Gilson, and G. I. McFadden. 2000. Mitochondrial FtsZ in a chromophyte alga. Science 287:1276-1279.
7. Berger, B. R., and P. J. Christie. 1994. Genetic complementation analysis of the Agrobacterium tumefaciens virB operon: virB2 through virB11 are essential virulence genes. J. Bacteriol. 176:3646-3660.
8. Bi, E. F., and J. Lutkenhaus. 1991. FtsZ ring structure associated with division in Escherichia coli. Nature 354:161-164.
9. Chen, J. C., D. S. Weiss, J. M. Ghigo, and J. Beckwith. 1999. Septal localization of FtsQ, an essential cell division protein in Escherichia coli. J. Bacteriol. 181:521-530.
10. Christie, P. J. 2001. Type IV secretion: intercellular transfer of macromolecules by systems ancestrally related to conjugation machines. Mol. Microbiol. 40:294-305.
11. Dang, T. A. T., and P. J. Christie. 1997. The VirB4 ATPase of Agrobacterium tumefaciens is a cytoplasmic membrane protein exposed at the periplasmic surface. J. Bacteriol. 179:453-462.
12. Das, A., and Y.-H. Xie. 2000. The Agrobacterium T-DNA transport pore proteins VirB8, VirB9, and VirB10 interact with one another. J. Bacteriol. 182:758-763.
13. Deng, W., L. Chen, W.-T. Peng, X. Liang, S. Sekiguchi, M. P. Gordon, and E. W. Nester. 1999. VirE1 is a specific molecular chaperone for the exported single-stranded-DNA-binding protein VirE2 in Agrobacterium. Mol. Microbiol. 31:1795-1807.
14. Dziejman, M., H. Kolmar, H. J. Fritz, and J. J. Mekalanos. 1999. ToxR co-operative interactions are not modulated by environmental conditions or periplasmic domain conformation. Mol. Microbiol. 31:305-317.
15. Dziejman, M., and J. J. Mekalanos. 1994. Analysis of membrane protein interaction: ToxR can dimerize the amino terminus of phage lambda repressor. Mol. Microbiol. 13:485-494.
16. Edwards, D. H., and J. Errington. 1997. The Bacillus subtilis DivIVA protein targets to the division septum and controls the site specificity of cell division. Mol. Microbiol. 24:905-915.
17. Edwards, D. H., H. B. Thomaides, and J. Errington. 2000. Promiscuous targeting of Bacillus subtilis cell division protein DivIVA to division sites in Escherichia coli and fission yeast. EMBO J. 19:2719-2727.
18. Fernandez, D., T. A. T. Dang, G. M. Spudich, X.-R. Zhou, B. R. Berger, and P. J. Christie. 1996. The Agrobacterium tumefaciens virB7 gene product, a proposed component of the T-complex transport apparatus, is a membrane-associated lipoprotein exposed at the periplasmic surface. J. Bacteriol. 178: 3156-3167.
19. Fernandez, D., G. M. Spudich, X.-R. Zhou, and P. J. Christie. 1996. The Agrobacterium tumefaciens VirB7 lipoprotein is required for stabilization of VirB proteins during assembly of the T-complex transport apparatus. J. Bacteriol. 178:3168-3176.
20. Hale, C. A., and P. A. de Boer. 1997. Direct binding of FtsZ to ZipA, an essential component of the septal ring structure that mediates cell division in E. coli. Cell 88:175-185.
21. Hale, C. A., and P. A. de Boer. 1999. Recruitment of ZipA to the septal ring of Escherichia coli is dependent on FtsZ and independent of FtsA. J. Bacteriol. 181:167-176.
22. Hays, L. B., Y. S. Chen, and J. C. Hu. 2000. Two-hybrid system for characterization of protein-protein interactions in E. coli. BioTechniques 29:288-290, 292, 294.
23. Hu, Chang-Deng, Y. Chinenov, and T. K. Kerppola. 2002. Visualization of interactions among bZIP and Rel family proteins in living cells using bimolecular fluorescence complementation. Mol. Cell 9:789-798.
24. Hu, J., M. Kornacker, and A. Hochschild. 2000. Escherichia coli one- and two-hybrid systems for the analysis and identification of protein-protein interactions. Methods 20:80-94.
25. Hu, J. C., E. K. O'Shea, P. S. Kim, and R. T. Sauer. 1990. Sequence requirements for coiled-coils: analysis with lambda repressor-GCN4 leucine zipper fusions. Science 250:1814-1822.
26. Karimova, G., A. Ullmann, and D. Ladant. 2000. A bacterial two-hybrid system that exploits a cAMP signaling cascade in Escherichia coli. Methods Enzymol. 328:59-73.
27. Kumar, R. B., Y. H. Xie, and A. Das. 2000. Subcellular localization of the Agrobacterium tumefaciens T-DNA transport pore proteins: VirB8 is essential for the assembly of the transport pore. Mol. Microbiol. 36:608-617.
28. Lai, E. M., O. Chesnokova, L. M. Banta, and C. I. Kado. 2000. Genetic and environmental factors affecting T-pilin export and T-pilus biogenesis in relation to flagellation of Agrobacterium tumefaciens. J. Bacteriol. 182:3705-3716.
29. Leeds, J. A., and J. Beckwith. 1998. Lambda repressor N-terminal DNA-binding domain as an assay for protein transmembrane segment interactions in vivo. J. Mol. Biol. 280:799-810.
30. Leeds, J. A., D. Boyd, D. R. Huber, G. K. Sonoda, H. T. Luu, D. M. Engelman, and J. Beckwith. 2001. Genetic selection for and molecular dynamic modeling of a protein transmembrane domain multimerization motif from a random Escherichia coli genomic library. J. Mol. Biol. 313:181-195.
31. Legrain, P., J. Wojcik, and J. M. Gauthier. 2001. Protein-protein interaction maps: a lead towards cellular functions. Trends Genet. 17:346-352.
32. Lutkenhaus, J., and S. G. Addinall. 1997. Bacterial cell division and the Z ring. Annu. Rev. Biochem. 66:93-116.
33. Ma, X., D. W. Ehrhardt, and W. Margolin. 1996. Colocalization of cell division proteins FtsZ and FtsA to cytoskeletal structures in living Escherichia coli cells by using green fluorescent protein. Proc. Natl. Acad. Sci. USA. 93:12998-13003.
34. Ma, X., and W. Margolin. 1999. Genetic and functional analyses of the conserved C-terminal core domain of Escherichia coli FtsZ. J. Bacteriol. 181:7531-7544.
35. Ma, X., Q. Sun, R. Wang, G. Singh, E. L. Jonietz, and W. Margolin. 1997. Interactions between heterologous FtsA and FtsZ proteins at the FtsZ ring. J. Bacteriol. 179:6788-6797.
36. Manoil, C., and J. Bailey. 1997. A simple screen for permissive sites in proteins: analysis of Escherichia coli lac permease. J. Mol. Biol. 267:250-263.
37. Margolin, W. 2000. Themes and variations in prokaryotic cell division. FEMS Microbiol. Rev. 24:531-548.
38. Marston, A. L., H. B. Thomaides, D. H. Edwards, M. E. Sharpe, and J. Errington. 1998. Polar localization of the MinD protein of Bacillus subtilis and its role in selection of the mid-cell division site. Genes Dev. 12:3419-3430.
39. McBride, K. E., and V. C. Knauf. 1988. Genetic analysis of the virE operon of the Agrobacterium Ti plasmid pTiA6. J. Bacteriol. 170:1430-1437.
40. Mileykovskaya, E., Q. Sun, W. Margolin, and W. Dowhan. 1998. Localization and function of early cell division proteins in filamentous Escherichia coli cells lacking phosphatidylethanolamine. J. Bacteriol. 180:4252-4257.
41. Rothfield, L., S. Justice, and J. Garcia-Lara. 1999. Bacterial cell division. Annu. Rev. Genet. 33:423-448.
42. Sambrook, J. E., F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
43. Struhl, K. 1989. Helix-turn-helix, zinc-finger, and leucine-zipper motifs for eukaryotic transcriptional regulatory proteins. Trends Biochem. Sci. 14:137-140.
44. Sun, Q., and W. Margolin. 1998. FtsZ dynamics during the division cycle of live Escherichia coli cells. J. Bacteriol. 180:2050-2056.
45. Sundberg, C. D., and W. Ream. 1999. The Agrobacterium tumefaciens chaperone-like protein, VirE1, interacts with VirE2 at domains required for single-stranded DNA binding and cooperative interaction. J. Bacteriol. 181: 6850-6855.
46. Toby, G. G., and E. A. Golemis. 2001. Using the yeast interaction trap and other two-hybrid-based approaches to study protein-protein interactions. Methods 24:201-217.
47. Truong, K., and M. Ikura. 2001. The use of FRET imaging microscopy to detect protein-protein interactions and protein conformational changes in vivo. Curr. Opin. Struct. Biol. 11:573-578.
48. Tucker, C. L., J. F. Gera, and P. Uetz. 2001. Towards an understanding of complex protein networks. Trends Cell Biol. 11:102-106.
49. Uetz, P., and R. E. Hughes. 2000. Systematic and large-scale two-hybrid screens. Curr. Opin. Microbiol. 3:303-308.
50. van Heeckeren, W. J., J. W. Sellers, and K. Struhl. 1992. Role of the conserved leucines in the leucine zipper dimerization motif of yeast GCN4. Nucleic Acids Res. 20:3721-3724.
51. Vitha, S., R. S. McAndrew, and K. W. Osteryoung. 2001. FtsZ ring formation at the chloroplast division site in plants. J. Cell Biol. 153:111-120.
52. Ward, J. E., Jr., E. M. Dale, E. W. Nester, and A. N. Binns. 1990. Identification of a VirB10 protein aggregate in the inner membrane of Agrobacterium tumefaciens. J. Bacteriol. 172:5200-5210.
53. Xu, Y., D. W. Piston, and C. H. Johnson. 1999. A bioluminescence resonance energy transfer (BRET) system: application to interacting circadian clock proteins. Proc. Natl. Acad. Sci. USA 96:151-156.
54. Zhao, Z., E. Sagulenko, Z. Ding, and P. J. Christie. 2001. Activities of virE1 and the VirE1 secretion chaperone in export of the multifunctional VirE2 effector via an Agrobacterium type IV secretion pathway. J. Bacteriol. 183: 3855-3865.
55. Zhou, X.-R., and P. J. Christie. 1999. Mutagenesis of Agrobacterium VirE2 single-stranded DNA-binding protein identifies regions required for self-association and interaction with VirE1 and a permissive site for hybrid protein construction. J. Bacteriol. 181:4342-4352.