EdU incorporation is an alternative non-radioactive assay to [3H]thymidine uptake for in vitro measurement of mice T-cell proliferations

Journal of Immunological Methods

Volumn 350, Issue 1-2, 2009, Pages 29-35

  Yu, Yongmao ^a   Arora, Alka ^a   Min, Weixian ^a   Roifman, Chaim M ^a   Grunebaum, Eyal ^a  

^a HOSPITAL FOR SICK CHILDREN   (Canada)

Author keywords

5 ethynyl 2 deoxyuridine; Click; Function; Immunodeficiency; Purine nucleoside phosphorylase; T lymphocytes; Thymidine

Indexed keywords

5 ETHYNYLDEOXYURIDINE; CD3 ANTIGEN; CD4 ANTIGEN; CUPROUS ION; DEOXYURIDINE DERIVATIVE; IMMUNOGLOBULIN M; PURINE NUCLEOSIDE PHOSPHORYLASE; THYMIDINE; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CELL ASSAY; CONTROLLED STUDY; DNA SYNTHESIS; FLOW CYTOMETRY; IN VITRO STUDY; LYMPHOCYTE PROLIFERATION; MOUSE; NONHUMAN; NUCLEIC ACID TRANSPORT; PRIORITY JOURNAL; PROTEIN EXPRESSION; SPLEEN CELL; T LYMPHOCYTE; T LYMPHOCYTE ACTIVATION;

ANIMALS; ANTIBODIES; ANTIGENS, CD3; CELL PROLIFERATION; COPPER; DEOXYURIDINE; FLOW CYTOMETRY; MICE; MICE, KNOCKOUT; PURINE-NUCLEOSIDE PHOSPHORYLASE; SPLEEN; T-LYMPHOCYTES; THYMIDINE; TRITIUM;

MURINAE; MUS;

EID: 70349729707     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2009.07.008     Document Type: Article

References (20)

1. Arpaia E., Benveniste P., Di Cristofano A., Gu Y., Dalal I., Kelly S., Hershfield M., Pandolfi P.P., Roifman C.M., and Cohen A. Evidence from purine nucleoside phosphorylase-deficient micenucleoside phosphorylase-deficient mice. J. Exp. Med. 191 (2000) 2197
2. Buck S.B., Bradford J., Gee K.R., Agnew B.J., Clarke S.T., and Salic A. Detection of S-phase cell cycle progression using 5-ethynyl-2′-deoxyuridine incorporation with click chemistry, an alternative to using 5-bromo-2′-deoxyuridine antibodies. Biotechniques 44 (2008) 927
3. Cappella P., Gasparri F., Pulici M., and Moll J. A novel method based on click chemistry, which overcomes limitations of cell cycle analysis by classical determination of BrdU incorporation, allowing multiplex antibody staining. Cytometry A 73 (2008) 626
4. Chehrehasa F., Meedeniya A.C., Dwyer P., Abrahamsen G., and Mackay-Sim A. EdU, a new thymidine analogue for labeling proliferating cells in the nervous system. J. Neurosci. Methods 177 (2009) 122
5. Goodell V., dela Rosa C., Slota M., MacLeod B., and Disis M.L. Sensitivity and specificity of tritiated thymidine incorporation and ELISPOT assays in identifying antigen specific T cell immune responses. BMC Immunol. 8 (2007) 21
6. Gratzner H.G. Monoclonal antibody to 5-bromo- and 5-iododeoxyuridine: a new reagent for detection of DNA replication. Science 218 (1982) 474
7. Grunebaum E., Mazzolari E., Porta F., Dallera D., Atkinson A., Reid B., Notarangelo L.D., and Roifman C.M. Bone marrow transplantation for severe combined immune deficiency. JAMA 295 (2006) 508
8. Kramer A., Jacobson S., Reuben J.F., Murphy E.L., Wiktor S.Z., Cranston B., Figueroa J.P., Hanchard B., McFarlin D., and Blattner W.A. Spontaneous lymphocyte proliferation in symptom-free HTLV-I positive Jamaicans. Lancet 2 (1989) 923
9. Liao P., Toro A., Min W., Lee S., Roifman C.M., and Grunebaum E. Lentivirus gene therapy for purine nucleoside phosphorylase deficiency. J. Gene Med. 10 (2008) 1282
10. Macallan D.C., Fullerton C.A., Neese R.A., Haddock K., Park S.S., and Hellerstein M.K. Measurement of cell proliferation by labeling of DNA with stable isotope-labeled glucose: studies in vitro, in animals, and in humans. Proc. Natl. Acad. Sci. U. S. A. 95 (1998) 708
11. Markert M.L. Purine nucleoside phosphorylase deficiency. Immunodefic. Rev. 3 (1991) 45
12. 3H]thymidine incorporation assay. Clin. Diagn. Lab. Immunol. 7 (2000) 687
13. Rakic P. Neurogenesis in adult primate neocortex: an evaluation of the evidence. Nat. Rev. Neurosci. 3 (2002) 65
14. Salic A., and Mitchison T.J. A chemical method for fast and sensitive detection of DNA synthesis in vivo. Proc. Natl. Acad. Sci. U. S. A. 105 (2008) 2415
15. Sawada S., Asakura S., Daimon H., and Furihata C. Comparison of autoradiography, liquid scintillation counting and immunoenzymatic staining of 5-bromo-2′-deoxyuridine for measurement of unscheduled DNA synthesis and replicative DNA synthesis in rat liver. Mutat. Res. 344 (1995) 109
16. Stone K.D., Feldman H.A., Huisman C., Howlett C., Jabara H.H., and Bonilla F.A. Analysis of in vitro lymphocyte proliferation as a screening tool for cellular immunodeficiency. Clin. Immunol. 131 (2009) 41
17. Taghon T., Van de Walle I., De Smet G., De Smedt M., Leclercq G., Vandekerckhove B., and Plum J. Notch signaling is required for proliferation but not for differentiation at a well-defined {beta}-selection checkpoint during human T cell development. Blood 113 (2009) 3254
18. Toro A., and Grunebaum E. TAT-mediated intracellular delivery of purine nucleoside phosphorylase corrects its deficiency in mice. J. Clin. Invest. 116 (2006) 2717
19. Waldman F.M., Chew K., Ljung B.M., Goodson W., Hom J., Duarte L.A., Smith H.S., and Mayall B. A comparison between bromodeoxyuridine and 3H thymidine labeling in human breast tumors. Mod. Pathol. 4 (1991) 718
20. Yu Y., Arura A., Min W., and Grunebaum E. EdU-Click iT flow cytometry as an alternative to [3H]Thymidine for measuring proliferation of human T lymphocytes. Allergy Asthma Clin. Immunol. S1 (2008) 22