Novel in vitro protein fragment complementation assay applicable to high-throughput screening in a 1536-well format

Journal of Biomolecular Screening

Volumn 14, Issue 8, 2009, Pages 970-979

  Hashimoto, Junko ^a   Watanabe, Taku ^a,b   Seki, Tatsuya ^a,b   Karasawa, Satoshi ^a,c   Izumikawa, Miho ^a   Seki, Tomoe ^a   Iemura, Shun Ichiro ^d   Natsume, Tohru ^d   Nomura, Nobuo ^d   Goshima, Naoki ^d   Miyawaki, Atsushi ^c   Takagi, Motoki ^a   Shin Ya, Kazuo ^d  

^a Koto ku   (Japan)

^b Amalgaam Co Ltd   (Japan)

^c Brain Science Institute   (Japan)

^d NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY AIST   (Japan)

Author keywords

Bimolecular fluorescence complementation; High throughput screening; Monomeric Kusabira Green protein; Protein fragment complementation assay; Protein protein interaction

Indexed keywords

ANTINEOPLASTIC AGENT; BETA CATENIN; DUAL SPECIFICITY PHOSPHATASE 2; GREEN FLUORESCENT PROTEIN; HOMODIMER; JBIR 22; LEUCANICIDIN; LINOLEIC ACID; LINOLENIC ACID; MONOMERIC KUSABIRA GREEN FLUORESCENT PROTEIN; NATURAL PRODUCT; OLEIC ACID; PROTEASOME; PROTEASOME ASSEMBLING CHAPERONE 2; PROTEASOME ASSEMBLING CHAPERONE 3; PROTEASOME INHIBITOR; SOYASAPONIN I; SOYASAPONIN II; T CELL FACTOR PROTEIN; T CELL FACTOR PROTEIN 7; TAN 1323D; TB 1; TN 01; UNCLASSIFIED DRUG;

ARTICLE; CANCER CELL; CANCER GROWTH; CONCENTRATION RESPONSE; CONTROLLED STUDY; DRUG PROTEIN BINDING; FRAGMENTATION REACTION; HIGH THROUGHPUT SCREENING; IC 50; IN VITRO STUDY; IN VIVO STUDY; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN FRAGMENT COMPLEMENTATION ASSAY; PROTEIN PROTEIN INTERACTION; PROTEIN SYNTHESIS; PROTEIN TARGETING; STRUCTURE ACTIVITY RELATION;

ANTINEOPLASTIC AGENTS; BINDING, COMPETITIVE; CELL-FREE SYSTEM; DRUG SCREENING ASSAYS, ANTITUMOR; HIGH-THROUGHPUT SCREENING ASSAYS; LUMINESCENT MEASUREMENTS; MODELS, BIOLOGICAL; PEPTIDE FRAGMENTS; PEPTIDES; PROTEIN BINDING; PROTEIN INTERACTION DOMAINS AND MOTIFS; PROTEIN INTERACTION MAPPING; SMALL MOLECULE LIBRARIES;

EID: 70349646101     PISSN: 10870571     EISSN: 1552454X     Source Type: Journal    
DOI: 10.1177/1087057109341406     Document Type: Article

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