A cDNA library functional screening strategy based on fluorescent protein complementation assays to identify novel components of signaling pathways

Methods

Volumn 32, Issue 4, 2004, Pages 381-388

  Remy, Ingrid ^a   Michnick, Stephen W ^a  

^a UNIVERSITÉ DE MONTRÉAL   (Canada)

Author keywords

cDNA library screening; Green fluorescent protein; Protein fragment complementation assay; Protein protein interactions

Indexed keywords

CELL PROTEIN; COMPLEMENTARY DNA; GREEN FLUORESCENT PROTEIN; REGULATOR PROTEIN;

ANALYTIC METHOD; ANIMAL CELL; ARTICLE; CONTROLLED STUDY; DNA LIBRARY; DNA SCREENING; ENZYME SPECIFICITY; FLUORESCENCE; GENE IDENTIFICATION; GENETIC COMPLEMENTATION; HUMAN; HUMAN CELL; NONHUMAN; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; REPRODUCIBILITY; SCREENING TEST; SIGNAL TRANSDUCTION; STATISTICAL SIGNIFICANCE; VALIDATION PROCESS;

1-PHOSPHATIDYLINOSITOL 3-KINASE; ANDROSTADIENES; ANIMALS; CELL LINE, TUMOR; CELL MEMBRANE; CERCOPITHECUS AETHIOPS; COS CELLS; CYTOSOL; GENE LIBRARY; GENES, REPORTER; GENETIC VECTORS; HUMANS; INSULIN; MICROSCOPY, FLUORESCENCE; PEPTIDE FRAGMENTS; PROTEIN BINDING; PROTEIN-SERINE-THREONINE KINASES; PROTEINS; SIGNAL TRANSDUCTION; SPECTROMETRY, FLUORESCENCE; TRANSFORMATION, GENETIC; TWO-HYBRID SYSTEM TECHNIQUES;

EID: 1542375250     PISSN: 10462023     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ymeth.2003.10.011     Document Type: Article

References (35)

1. D'Andrea A.D., Lodish H.F., Wong G.G. Cell. 57:1989;277-285.
2. Lin H.Y., Wang X.F., Ng-Eaton E., Weinberg R.A., Lodish H.F. Cell. 68:1992;775-785.
3. Aruffo A., Seed B. Proc. Natl. Acad. Sci. USA. 84:1987;8573-8577.
4. Sako D., Chang X.J., Barone K.M., Vachino G., White H.M., Shaw G., Veldman G.M., Bean K.M., Ahern T.J., Furie B. Cell. 75:1993;1179-1186.
5. Pawson T., Nash P. Genes. Dev. 14:2000;1027-1047.
6. Weston C.R., Davis R.J. Science. 292:2001;2439-2440.
7. Drees B.L. Curr. Opin. Chem. Biol. 3:1999;64-70.
8. Vidal M., Legrain P. Nucleic Acids Res. 27:1999;919-929.
9. Walhout A.J., Sordella R., Lu X., Hartley J.L., Temple G.F., Brasch M.A., Thierry-Mieg N., Vidal M. Science. 287:2000;116-122.
10. Uetz P., Giot L., Cagney G., Mansfield T.A., Judson R.S., Knight J.R., Lockshon D., Narayan V., Srinivasan M., Pochart P., Qureshi-Emili A., Li Y., Godwin B., Conover D., Kalbfleisch T., Vijayadamodar G., Yang M., Johnston M., Fields S., Rothberg J.M. Nature. 403:2000;623-627.
11. Fields S., Song O. Nature. 340:1989;245-246.
12. Ito T., Tashiro K., Muta S., Ozawa R., Chiba T., Nishizawa M., Yamamoto K., Kuhara S., Sakaki Y., Giaever G., Chu A.M., Ni L., Connelly C., Riles L., Veronneau S., Dow S., Lucau-Danila A., Anderson K., Andre B., Arkin A.P., Astromoff A., El-Bakkoury M., Bangham R., Benito R., Brachat S., Campanaro S., Curtiss M., Davis K., Deutschbauer A., Entian K.D., Flaherty P., Foury F., Garfinkel D.J., Gerstein M., Gotte D., Guldener U., Hegemann J.H., Hempel S., Herman Z., Jaramillo D.F., Kelly D.E., Kelly S.L., Kotter P., LaBonte D., Lamb D.C., Lan N., Liang H., Liao H., Liu L., Luo C., Lussier M., Mao R., Menard P., Ooi S.L., Revuelta J.L., Roberts C.J., Rose M., Ross-Macdonald P., Scherens B., Schimmack G., Shafer B., Shoemaker D.D., Sookhai-Mahadeo S., Storms R.K., Strathern J.N., Valle G., Voet M., Volckaert G., Wang C.Y., Ward T.R., Wilhelmy J., Winzeler E.A., Yang Y., Yen G., Youngman E., Yu K., Bussey H., Boeke J.D., Snyder M., Philippsen P., Davis R.W., Johnston M. Proc. Natl. Acad. Sci. USA. 97:2000;1143-1147.
13. Ito T., Chiba T., Ozawa R., Yoshida M., Hattori M., Sakaki Y., Zhang J., Ma Y., Taylor S.S., Tsien R.Y. Proc. Natl. Acad. Sci. USA. 98:2001;4569-4574.
14. Kaufman R.J., Davies M.V., Pathak V.K., Hershey J.W. Mol. Cell. Biol. 9:1989;946-958.
15. Michnick S.W., Remy I., Valois F.X.C., Belisle A.V., Pelletier J.N. Abelson J.N., Emr S.D., Thorner J. Methods in Enzymology. vol. 328:2000;208-230 Academic Press, New York.
16. Remy I., Pelletier J.N., Galarneau A., Michnick S.W. Golemis E.A. Protein-Protein Interactions: A Molecular Cloning Manual. 2001;449-475 Cold Spring Harbor Laboratory Press.
17. Ghosh I., Hamilton A.D., Regan L. J. Am. Chem. Soc. 2000;5658-5659.
18. Hu C.D., Chinenov Y., Kerppola T.K. Mol. Cell. 9:2002;789-798.
19. Remy I., Michnick S.W. Proc. Natl. Acad. Sci. USA. 98:2001;7678-7683.
20. Remy I., Michnick S.W. Proc. Natl. Acad. Sci. USA. 96:1999;5394-5399.
21. Remy I., Wilson I.A., Michnick S.W. Science. 283:1999;990-993.
22. Subramaniam R., Desveaux D., Spickler C., Michnick S.W., Brisson N. Nat. Biotechnol. 19:2001;769-772.
23. Brazil D.P., Hemmings B.A. Trends Biochem. Sci. 26:2001;657-664.
24. Datta S.R., Brunet A., Greenberg M.E. Genes Dev. 13:1999;2905-2927.
25. Kandel E.S., Hay N. Exp. Cell Res. 253:1999;210-229.
26. Lawlor M.A., Alessi D.R. J. Cell Sci. 114:2001;2903-2910.
27. Brazil D.P., Park J., Hemmings B.A. Cell. 111:2002;293-303.
28. van der Hoeven F., Schimmang T., Volkmann A., Mattei M.G., Kyewski B., Ruther U. Development. 120:1994;2601-2607.
29. Lesche R., Peetz A., van der Hoeven F., Ruther U. Mamm. Genome. 8:1997;879-883.
30. Belham C., Wu S., Avruch J. Curr. Biol. 9:1999;R93-R96.
31. Peterson R.T., Schreiber S.L. Curr. Biol. 9:1999;R521-R524.
32. Vanhaesebroeck B., Alessi D.R. Biochem. J. 3:2000;561-576.
33. Johnsson N., Varshavsky A. Proc. Natl. Acad. Sci. USA. 91:1994;10340-10344.
34. Laser H., Bongards C., Schuller J., Heck S., Johnsson N., Lehming N. Proc. Natl. Acad. Sci. USA. 97:2000;13732-13737.
35. Zhang J., Lautar S. Anal. Biochem. 242:1996;68-72.