The trypanosome rab-related proteins RabX1 and RabX2 play no role in intracellular trafficking but may be involved in fly infectivity

PLoS ONE

Volumn 4, Issue 9, 2009, Pages

  Natesan, Senthil Kumar A ^a   Peacock, Lori ^b   Leung, Kai Fai ^a   Matthews, Keith R ^c   Gibson, Wendy ^b   Field, Mark C ^a  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^b UNIVERSITY OF BRISTOL   (United Kingdom)

^c UNIVERSITY OF EDINBURGH   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

GENE PRODUCT; PROTEIN RABX1; PROTEIN RABX2; RAB PROTEIN; RNA; UNCLASSIFIED DRUG; PROTOZOAL PROTEIN;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; CELL COMPARTMENTALIZATION; CELL DIFFERENTIATION; CELL LINE; CELL SURVIVAL; COMPARATIVE GENOMIC HYBRIDIZATION; CONTROLLED STUDY; ENDOCYTOSIS; EXOCYTOSIS; FEMALE; INTRACELLULAR TRANSPORT; KNOCKOUT GENE; MALE; MOUSE; NONHUMAN; PROTEIN FUNCTION; PROTEIN LOCALIZATION; PROTOZOAL INFECTION; RNA INTERFERENCE; TRYPANOSOMA; ANIMAL; CELL MEMBRANE; ENDOPLASMIC RETICULUM; GENETIC PROCEDURES; GOLGI COMPLEX; METABOLISM; PHYSIOLOGY; PROTEIN TRANSPORT; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TRYPANOSOMA BRUCEI; TRYPANOSOMIASIS; TSETSE FLY;

PROTOZOA; TRYPANOSOMA BRUCEI; TRYPANOSOMATIDAE;

ANIMALS; CELL DIFFERENTIATION; CELL MEMBRANE; ENDOPLASMIC RETICULUM; FEMALE; GENETIC TECHNIQUES; GOLGI APPARATUS; MALE; MICE; PROTEIN TRANSPORT; PROTOZOAN PROTEINS; RAB GTP-BINDING PROTEINS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA INTERFERENCE; TRYPANOSOMA BRUCEI BRUCEI; TRYPANOSOMIASIS; TSETSE FLIES;

EID: 70349640626     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0007217     Document Type: Article

References (55)

1. Barrett MP, Burchmore RJ, Stich A, Lazzari JO, Frasch AC, et al. (2003) The trypanosomiases. Lancet 362: 1469-1480.
2. Natesan SK, Peacock L, Matthews K, Gibson W, Field MC (2007) Activation of endocytosis as an adaptation to the mammalian host by trypanosomes. Eukaryot Cell 6: 2029-2037.
3. Reuner B, Vassella E, Yutzy B, Boshart M (1997) Cell density triggers slender to stumpy differentiation of Trypanosoma brucei bloodstream forms in culture. Mol Biochem Parasitol 90: 269-280.
4. Vassella E, Reuner B, Yutzy B, Boshart M (1997) Differentiation of African trypanosomes is controlled by a density sensing mechanism which signals cell cycle arrest via the cAMP pathway. J Cell Sci 110: 2661-2671.
5. Matthews KR (2005) The developmental cell biology of Trypanosoma brucei. J Cell Sci 118: 283-290.
6. Vickerman K, Tetley L, Hendry KA, Turner CM (1988) Biology of African trypanosomes in the tsetse fly. Biol Cell 64: 109-119.
7. Welburn SC, Maudlin I (1999) Tsetse-trypanosome interactions: rites of passage. Parasitol Today 15: 399-403.
8. Herder S, Votypka J, Jirku M, Radrova J, Janzen CJ, et al. (2007) Trypanosoma brucei 29-13 strain is inducible in but not permissive for the tsetse fly vector. Exp Parasitol 117: 111-114.
9. Maudlin I (1991) Transmission of African trypanosomiasis: Interactions among tsetse immune system, symbionts and parasties. Advances in Disease Vector Research 7: 117-148.
10. Peacock L, Ferris V, Bailey M, Gibson W (2008) Fly transmission and mating of Trypanosoma brucei brucei strain 427. Mol Biochem Parasitol 160: 100-106.
11. Field MC, Carrington M (2004) Intracellular membrane transport systems in Trypanosoma brucei. Traffic 5: 905-913.
12. Morgan GW, Allen CL, Jeffries TR, Hollinshead M, Field MC (2001) Developmental and morphological regulation of clathrin-mediated endocytosis in Trypanosoma brucei. J Cell Sci 114: 2605-2615.
13. Barry JD (1979) Capping of variable antigen on Trypanosoma brucei, and its immunological and biological significance. J Cell Sci 37: 287-302.
14. Engstler M, Pfohl T, Herminghaus S, Boshart M, Wiegertjes G, et al. (2007) Hydrodynamic flow-mediated protein sorting on the cell surface of trypanosomes. Cell 131: 505-515.
15. Pal A, Hall BS, Nesbeth DN, Field HI, Field MC (2002) Differential endocytic functions of Trypanosoma brucei Rab5 isoforms reveal a glycosylphosphatidylinositol-specific endosomal pathway. J Biol Chem 277: 9529-9539.
16. Morgan GW, Hall BS, Denny PW, Carrington M, Field MC (2002) The kinetoplastida endocytic apparatus. Part I: a dynamic system for nutrition and evasion of host defences. Trends Parasitol 18: 491-496.
17. Morgan GW, Hall BS, Denny PW, Field MC, Carrington M (2002) The endocytic apparatus of the kinetoplastida. Part II: machinery and components of the system. Trends Parasitol 18: 540-546.
18. Ackers JP, Dhir V, Field MC (2005) A bioinformatic analysis of the RAB genes of Trypanosoma brucei. Mol Biochem Parasitol 141: 89-97.
19. Field MC (2005) Signalling the genome: the Ras-like small GTPase family of trypanosomatids. Trends Parasitol 21: 447-450.
20. Dacks JB, Peden AA, Field MC (2009) Evolution of specificity in the eukaryotic endomembrane system. Int J Biochem Cell Biol 41: 330-340.
21. Berriman M, Ghedin EE, Hertz-Fowler C, Blandin G, Renauld H, et al. (2005) The genome of the African trypanosome Trypanosoma brucei. Science 309: 416-422.
22. Field MC, Gabernet-Castello C, Dacks JB (2007) Reconstructing the evolution of the endocytic system: insights from genomics and molecular cell biology. Adv Exp Med Biol 607: 84-96.
23. Dhir V, Goulding D, Field MC (2004) TbRAB1 and TbRAB2 mediate trafficking through the early secretory pathway of Trypanosoma brucei. Mol Biochem Parasitol 137: 253-265.
24. Field H, Farjah M, Pal A, Gull K, Field MC (1998) Complexity of trypanosomatid endocytosis pathways revealed by Rab4 and Rab5 isoforms in Trypanosoma brucei. J Biol Chem 273: 32102-32110.
25. Jeffries TR, Morgan GW, Field MC (2001) A developmentally regulated rab11 homologue in Trypanosoma brucei is involved in recycling processes. J Cell Sci 114: 2617-2626.
26. Denny PW, Lewis S, Tempero JE, Goulding D, Ivens AC, et al. (2002) Leishmania RAB7: characterisation of terminal endocytic stages in an intracellular parasite. Mol Biochem Parasitol 123: 105-113.
27. Seabra MC, Mules EH, Hume AN (2002) Rab GTPases, intracellular traffic and disease. Trends Mol Med 8: 23-30.
28. Field H, Field MC (1997) Tandem duplication of rab genes followed by sequence divergence and acquisition of distinct functions in Trypanosoma brucei. J Biol Chem 272: 10498-10505.
29. Field H, Sherwin T, Smith AC, Gull K, Field MC (2000) Cell-cycle and developmental regulation of TbRAB31 localisation, a GTP-locked Rab protein from Trypanosoma brucei. Mol Biochem Parasitol 106: 21-35.
30. Brun R, Schonenberger (1979) Cultivation and in vitro cloning or procyclic culture forms of Trypanosoma brucei in a semi-defined medium. Short communication. Acta Trop 36: 289-292.
31. Hirumi H, Hirumi K (1994) Axenic culture of African trypanosome bloodstream forms. Parasitol Today 10: 80-84.
32. Herbert WJ, Lumsden WH (1976) Trypanosoma brucei: a rapid "matching" method for estimating the host's parasitemia. Exp Parasitol 40: 427-431.
33. Macleod ET, Darby AC, Maudlin I, Welburn SC (2007) Factors affecting trypanosome maturation in tsetse flies. PLoS ONE 2: e239.
34. Redmond S, Vadivelu J, Field MC (2003) RNAit: an automated web-based tool for the selection of RNAi targets in Trypanosoma brucei. Mol Biochem Parasitol 128: 115-118.
35. Quijada, L, Guerra-Giraldez C, Drozdz M, Hartmann C, Irmer H, et al. (2002) Expression of the human RNA-binding protein HuR in Trypanosoma brucei increases the abundance of mRNAs containing AU-rich regulatory elements. Nucleic Acids Res 30: 4414-4424.
36. Medina-Acosta E, Cross GA (1993) Rapid isolation of DNA from trypanosomatid protozoa using a simple 'mini-prep' procedure. Mol Biochem Parasitol 59: 327-329.
37. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular Cloning, a Laboratory Manual. Cold Spring Harbour, New York: Cold Spring Harbour Laboratory Press.
38. Field MC, Allen CL, Dhir V, Goulding D, Hall BS, et al. (2004) New approaches to the microscopic imaging of Trypanosoma brucei. Microsc Microanal 10: 621-636.
39. Skepper JN (2000) Immunocytochemical strategies for electron microscopy: choice or compromise. J Microsc 199: 1-36.
40. Allen CL, Goulding D, Field MC (2003) Clathrin-mediated endocytosis is essential in Trypanosoma brucei. EMBO J 22: 4991-5002.
41. Field H, Ali BR, Sherwin T, Gull K, Croft SL, et al. (1999) TbRab2p, a marker for the endoplasmic reticulum of Trypanosoma brucei, localises to the ERGIC in mammalian cells. J Cell Sci 112 (Pt 2): 147-156.
42. Field MC, O'Reilly AJ (2008) How complex is GTPase signaling in trypanosomes? Trends Parasitol 24: 253-257.
43. Hall B, Allen CL, Goulding D, Field MC (2004) Both of the Rab5 subfamily small GTPases of Trypanosoma brucei are essential and required for endocytosis. Mol Biochem Parasitol 138: 67-77.
44. Dean S, Marchetti R, Kirk K, Matthews KR (2009) A surface transporter family conveys the trypanosome differentiation signal. Nature 459: 213-7.
45. Schell D, Borowy NK, Overath P (1991) Transferrin is a growth factor for the bloodstream form of Trypanosoma brucei. Parasitol Res 77: 558-560.
46. Steverding D, Stierhof YD, Fuchs H, Tauber R, Overath P (1995) Transferrin-binding protein complex is the receptor for transferrin uptake in Trypanosoma brucei. J Cell Biol 131: 1173-1182.
47. Hall BS, Pal A, Goulding D, Field MC (2004) Rab4 is an essential regulator of lysosomal trafficking in trypanosomes. J Biol Chem 279: 45047-45056.
48. Hall BS, Smith E, Langer W, Jacobs LA, Goulding D, et al. (2005) Developmental variation in Rab11-dependent trafficking in Trypanosoma brucei. Eukaryot Cell 4: 971-980.
49. Field MC, Boothroyd JC (1995) Trypanosoma brucei: molecular cloning of homologues of small GTP-binding proteins involved in vesicle trafficking. Exp Parasitol 81: 313-320.
50. Hendriks EF, Matthews KR (2005) Disruption of the developmental programme of Trypanosoma brucei by genetic ablation of TbZFP1, a differentiation-enriched CCCH protein. Mol Microbiol 57: 706-716.
51. Alexander DL, Schwartz KJ, Balber AE, Bangs JD (2002) Developmentally regulated trafficking of the lysosomal membrane protein p67 in Trypanosoma brucei. J Cell Sci 115: 3253-3263.
52. Bangs JD, Brouch EM, Ransom DM, Roggy JL (1996) A soluble secretory reporter system in Trypanosoma brucei. Studies on endoplasmic reticulum targeting. J Biol Chem 271: 18387-18393.
53. Bangs JD, Uyetake L, Brickman MJ, Balber AE, Boothroyd JC (1993) Molecular cloning and cellular localization of a BiP homologue in Trypanosoma brucei. Divergent ER retention signals in a lower eukaryote. J Cell Sci 105 (Pt 4): 1101-1113.
54. Triggs VP, Bangs JD (2003) Glycosylphosphatidylinositol-dependent protein trafficking in bloodstream stage Trypanosoma brucei. Eukaryot Cell 2: 76-83.
55. Szoor B, Wilson J, McElhinney H, Tabernero L, Matthews KR (2006) Protein tyrosine phosphatase TbPTP1: A molecular switch controlling life cycle differentiation in trypanosomes. J Cell Biol 175: 293-303.