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Volumn 81, Issue 19, 2009, Pages 7908-7916

Effective enhancement of fluorescence detection efficiency in protein microarray assays: Application of a highly fluorinated organosilane as the blocking agent on the background surface by a facile vapor-phase deposition process

Author keywords

[No Author keywords available]

Indexed keywords

BACKGROUND NOISE; BINDING EFFICIENCY; BLOCKING AGENTS; BLOCKING PROCESS; BLOCKING TIME; CROSS CONTAMINATION; ENABLING TECHNOLOGIES; FLUORESCENCE DETECTION; MODIFIED SURFACES; ORGANOSILANES; PROTEIN BINDING; PROTEIN MICROARRAY; S/N RATIO; SELF-ASSEMBLED; SURFACE COATINGS; VAPOR PHASE; VAPOR PHASE DEPOSITION;

EID: 70349631662     PISSN: 00032700     EISSN: None     Source Type: Journal    
DOI: 10.1021/ac900552v     Document Type: Article
Times cited : (31)

References (45)
  • 37
    • 70349619250 scopus 로고    scopus 로고
    • note
    • During the measurements of FTIR spectra, we need to measure absorbance at the same position after each silanization modification and subtract the absorbance obtained before modification. Otherwise, even the slight surface roughness within 1 nm or disordered surface modification quality will interfere with the measurements. Since the self-assembled coating merely has several nanometers in thickness, it shows relative weak absorbance signal compared to that from the substrate. If the measurement is done by scanning at different locations on the same sample surface or on different silane-modified substrates, the fine change corresponding to each surface modification may not be recognized in the spectra.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.