Use of protein-acrylamide copolymer hydrogels for measuring protein concentration and activity

Analytical Biochemistry

Volumn 329, Issue 2, 2004, Pages 180-189

  Brueggemeier, Shawn B ^a   Kron, Stephen J ^b   Palecek, Sean P ^a  

^a University of Wisconsin Madison   (United States)

^b UNIVERSITY OF CHICAGO   (United States)

Author keywords

c Src; Copolymerization; Enzymes; Immunoassay; Polyacrylamide; Protein array; Tyrosine kinase

Indexed keywords

ACRYLIC MONOMERS; AMIDES; AMINO ACIDS; BINDING ENERGY; CHEMICAL DETECTION; COPOLYMERIZATION; ENZYME IMMOBILIZATION; ENZYME KINETICS; ENZYMES; FLUORESCENCE; KINETICS; PLANNING; POLYACRYLATES; PROTEOMICS; RATE CONSTANTS;

CONCENTRATION DETECTIONS; GLUTATHIONE S-TRANSFERASES; GREEN FLUORESCENT PROTEIN; IMMUNOASSAY; MICHAELIS-MENTEN KINETIC; PROTEIN ARRAYS; PROTEIN IMMOBILIZATION; TYROSINE KINASE;

SURFACE REACTIONS;

ACRYLAMIDE; ACRYLIC ACID; ANTIBODY; COPOLYMER; GLASS; GLUTATHIONE TRANSFERASE; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; POLYACRYLAMIDE; PROTEIN TYROSINE KINASE;

ARTICLE; BINDING AFFINITY; CHEMICAL REACTION; ENZYME KINETICS; ENZYME PHOSPHORYLATION; ENZYME SUBSTRATE; HYDROGEL; MICHAELIS CONSTANT; POLYMERIZATION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN DETERMINATION; PROTEIN IMMOBILIZATION;

EID: 2542505709     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ab.2004.02.012     Document Type: Article

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