Cloning, high expression and purification of recombinant human intereferon-β-1b in escherichia coli

Applied Biochemistry and Biotechnology

Volumn 158, Issue 1, 2009, Pages 140-154

  Krishna Rao, Dasari Venkata ^a   Tulasi Ramu, Chatadi ^a   Venkateswara Rao, Joginapally ^b   Lakshmi Narasu, Mangamoori ^b   Bhujanga Rao, Adibhatla Kali Satya ^a  

^a NATCO   (India)

^b JAWAHARLAL NEHRU TECHNOLOGICAL UNIVERSITY   (India)

Author keywords

High purity; High level expression; Recombinant human IFN 1b; Site directed mutagenesis; Specific product yield

Indexed keywords

BED HEIGHT; CELLULAR PROTEINS; CHROMATOGRAPHY COLUMNS; CODON SUBSTITUTIONS; CONCENTRATION OF; CULTURE CONDITIONS; DEOXYRIBONUCLEIC ACIDS; DIAFILTRATION; HIGH LEVEL EXPRESSION; HIGH PURITY; HOST CELL PROTEIN; IMPURITY PROFILE; KEY PARAMETERS; LIPOPOLYSACCHARIDES; N-TERMINALS; OPTICAL DENSITIES; PROCESS CONTROL STRATEGIES; PROTEIN REFOLDING; PURIFIED PROTEIN; REFOLDING; SITE DIRECTED MUTAGENESIS; SPECIFIC PRODUCT YIELD; THEORETICAL PLATES; TRITON X-100;

CELL CULTURE; CHROMATOGRAPHIC ANALYSIS; CLONING; COLUMN CHROMATOGRAPHY; DNA; ESCHERICHIA COLI; GENES; MUTAGENESIS; ORGANIC ACIDS; PURIFICATION; SULFUR COMPOUNDS; UREA;

PROTEINS;

BOVINE SERUM ALBUMIN; CELL PROTEIN; DNA; INTERFERON BETA SERINE; LIPOPOLYSACCHARIDE; PLASMID VECTOR; TRITON X 100; UREA; BETA INTERFERON; GLUCOSE; INTERFERON BETA-1B; ISOPROPYL THIOGALACTOSIDE; RECOMBINANT PROTEIN; RIFAMPICIN;

ARTICLE; CHROMATOGRAPHY; CODON; DIAFILTRATION; ESCHERICHIA COLI; GEL FILTRATION CHROMATOGRAPHY; GENE EXPRESSION SYSTEM; HUMAN; HYPERBARISM; MOLECULAR CLONING; NONHUMAN; PROCESS CONTROL; PURIFICATION; REVERSED PHASE LIQUID CHROMATOGRAPHY; SOLUBILIZATION; SUBSTITUTION REACTION; TOXICOLOGY; BIOSYNTHESIS; CHEMISTRY; CULTURE MEDIUM; CYTOLOGY; DOSE RESPONSE; DRUG EFFECT; GEL CHROMATOGRAPHY; GENE EXPRESSION; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; PRESSURE; PROTEIN RENATURATION; SOLUBILITY;

ESCHERICHIA COLI;

CHROMATOGRAPHY, GEL; CLONING, MOLECULAR; CULTURE MEDIA; DOSE-RESPONSE RELATIONSHIP, DRUG; ESCHERICHIA COLI; GENE EXPRESSION; GLUCOSE; HUMANS; INTERFERON-BETA; ISOPROPYL THIOGALACTOSIDE; PRESSURE; PROTEIN RENATURATION; RECOMBINANT PROTEINS; RIFAMPIN; SOLUBILITY;

EID: 70349575006     PISSN: 02732289     EISSN: None     Source Type: Journal    
DOI: 10.1007/s12010-008-8318-9     Document Type: Article

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