Function of host proteins in the agrobacterium-mediated plant transformation process

Agrobacterium: From Biology to Biotechnology

Volumn , Issue , 2008, Pages 483-522

  Gelvin, Stanton B ^a  

^a PURDUE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 70349507551     PISSN: None     EISSN: None     Source Type: Book    
DOI: 10.1007/978-0-387-72290-0_13     Document Type: Chapter

References (194)

1. Abuodeh RO, Orbach MJ, Mandel MA, Das A, Galgiani JN (2000) Genetic transformation of Coccidioides immitis facilitated by Agrobacterium tumefaciens. J Infect Dis 181: 2106-2110
2. Akama K, Shiraishi H, Ohta S, Nakamura K, Okada K, Shimura Y (1992) Efficient transformation of Arabidopsis thaliana: Comparison of the efficiencies with various organs, plant ecotypes and Agrobacterium strains. Plant Cell Rep 12: 7-11
3. An G, Lee S, Kim S-H, Kim S-R (2005) Molecular genetics using T-DNA in rice. Plant Cell Physiol 46: 14-22
4. An S, Park S, Jeong DH, Lee DY, Kang HG, Yu JH, Hur J, Kim SR, Kim YH, Lee M, Han S, Kim SJ, Yang J, Kim E, Wi SJ, Chung HS, Hong JP, Choe V, Lee HK, Choi JH, Nam J, Park PB, Park KY, Kim WT, Choe S, Lee CB, An G (2003) Generation and analysis of end sequence database for T-DNA tagging lines in rice. Plant Physiol 133: 2040-2047
5. Anderson AR, Moore LW (1979) Host specificity in the genus Agrobacterium. Phytopathology 69: 320-323
6. Bailey MA, Boerma HR, Parrott WA (1994) Inheritance of Agrobacterium tume-faciens-induced tumorigenesis of soybean. Crop Sci 34: 514-519
7. Bakó L, Umeda M, Tiburcio AF, Schell J, Koncz C (2003) The VirD2 pilot protein of Agrobacterium-transferred DNA interacts with the TATA box-binding protein and a nuclear protein kinase in plants. Proc Natl Acad Sci USA 100: 10108-10113
8. Ballas N, Citovsky V (1997) Nuclear localization signal binding protein from Arabidopsis mediates nuclear import of Agrobacterium VirD2 protein. Proc Natl Acad Sci USA 94: 10723-10728
9. Baron C, Zambryski PC (1996) Plant transformation: a pilus in Agrobacterium T-DNA transfer. Curr Biol 6: 1567-1569
10. Bechtold N, Ellis J, Pelletier G (1993) In planta Agrobacterium mediated gene transfer by infiltration of adult Arabidopsis thaliana plants. C R Acad Sci 316: 1194-1199
11. Bechtold N, Jaudeau B, Jolivet S, Maba B, Vezon D, Voisin R, Pelletier G (2000) The maternal chromosome set is the target of the T-DNA in the in planta transformation of Arabidopsis thaliana. Genetics 155: 1875-1887
12. Bechtold N, Jolivet S, Voisin R, Pelletier G (2003) The endosperm and the embryo of Arabidopsis thaliana are independently transformed through infiltration by Agrobacterium tumefaciens. Transgenic Res 12: 509-517
13. Bent AF (2000) Arabidopsis in planta transformation. Uses, mechanisms, and prospects for transformation of other species. Plant Physiol 124: 1540-1547
14. Binns AN, Beaupre CE, Dale EM (1995) Inhibition of VirB-mediated transfer of diverse substrates from Agrobacterium tumefaciens by the IncQ plasmid RSF1010. J Bacteriol 177: 4890-4899
15. Bliss FA, Almehdi AA, Dandekar AM, Schuerman PL, Bellaloui N (1999) Crown gall resistance in accessions of 20 Prunus species. Hort Sci 34: 326-330
16. Brencic A, Angert ER, Winans SC (2005) Unwounded plants elicit Agrobacterium vir gene induction and T-DNA transfer: transformed plant cells produce opines yet are tumour free. Mol Microbiol 57:1522-1531
17. Brencic A, Winans SC (2005) Detection of and response to signals involved in host-microbe interactions by plant-associated bacteria. Microbiol Mol Biol Rev 69: 155-194
18. Bundock P, den Dulk-Ras A, Beijersbergen A, Hooykaas PJJ (1995) Trans-kingdom T-DNA transfer from Agrobacterium tumefaciens to Saccharomyces cerevisiae. EMBO J 14: 3206-3214
19. Bundock P, Hooykaas PJJ (1996) Integration of Agrobacterium tumefaciens T-DNA in the Saccharomyces cerevisiae genome by illegitimate recombination. Proc Natl Acad Sci USA 93: 15272-15275
20. Bundock P, van Attikum H, den Dulk-Ras A, Hooykaas PJJ (2002) Insertional mutagenesis in yeasts using T-DNA from Agrobacterium tumefaciens. Yeast 19: 529-536
21. Bytebier B, Deboeck F, De Greve H, Van Montagu M, Hernalsteens JP (1987) T-DNA organization in tumor cultures and transgenic plants of the monocotyledon Asparagus officinalis. Proc Natl Acad Sci USA 84: 5345-5349
22. Cangelosi GA, Hung L, Puvanesarajah V, Stacey G, Ozga DA, Leigh JA, Nester EW (1987) Common loci for Agrobacterium tumefaciens and Rhizobium meliloti exopolysaccharide synthesis and their roles in plant interactions. J Bacteriol 169: 2086-2091
23. Cangelosi GA, Martinetti G, Leigh JA, Lee CC, Theines C, Nester EW (1989) Role for [corrected] Agrobacterium tumefaciens ChvA protein in export of beta-1, 2-glucan. J Bacteriol 171: 1609-1615
24. Cascales E, Christie PJ (2004) Definition of a bacterial type IV secretion pathway for a DNA substrate. Science 304: 1170-1173
25. Chan M-T, Lee T-M, Chang H-H (1992) Transformation of Indica rice (Oryza sa-tiva L.) mediated by Agrobacterium tumefaciens. Plant Cell Physiol 33: 577
26. Chen S, Jin W, Wang M, Zhang F, Zhou J, Jia Q, Wu Y, Liu F, Wu P (2003) Distribution and characterization of over 1000 T-DNA tags in rice genome. Plant J 36: 105-113
27. Cheng M, Fry JE, Pang S, Zhou H, Hironaka CM, Duncan DR, Conner TW, Wan Y (1997) Genetic transformation of wheat mediated by Agrobacterium tume-faciens. Plant Physiol 115: 971-980
28. Chilton M-D, Que Q (2003) Targeted integration of T-DNA into the tobacco genome at double-stranded breaks: new insights on the mechanism of T-DNA integration. Plant Physiol 133: 956-965
29. Christie PJ, Vogel JP (2000) Bacterial type IV secretion: conjugation systems adapted to deliver effector molecules to host cells. Trends Microbiol 8: 354-360
30. Christie PJ, Ward JE, Winans SC, Nester EW (1988) The Agrobacterium tumefa-ciens virE2 gene product is a single-stranded-DNA-binding protein that associates with T-DNA. J Bacteriol 170: 2659-2667
31. Citovsky V, De Vos G, Zambryski P (1988) Single-stranded DNA binding protein encoded by the virE locus of Agrobacterium tumefaciens. Science 240: 501-504
32. Citovsky V, Guralnick B, Simon MN, Wall JS (1997) The molecular structure of Agrobacterium VirE2-single stranded DNA complexes involved in nuclear import. J Mol Biol 271: 718-727
33. Citovsky V, Warnick D, Zambryski P (1994) Nuclear import of Agrobacterium VirD2 and VirE2 proteins in maize and tobacco. Proc Natl Acad Sci USA 91: 3210-3214
34. Citovsky V, Wong ML, Zambryski P (1989) Cooperative interaction of Agrobac-terium VirE2 protein with single stranded DNA: implications for the T-DNA transfer process. Proc Natl Acad Sci USA 86: 1193-1197
35. Citovsky V, Zupan J, Warnick D, Zambryski P (1992) Nuclear localization of Agrobacterium VirE2 protein in plant cells. Science 256: 1802-1805
36. Clough SJ, Bent AF (1998) Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J 16: 735-743
37. Conner AJ, Dommisse EM (1992) Monocotyledonous plants as hosts for Agro-bacterium. Int J Plant Sci 153: 550-555
38. Crews JL, Colby S, Matthysse AG (1990) Agrobacterium rhizogenes mutants that fail to bind to plant cells. J Bacteriol 172: 6182-6188
39. Das A (1988) Agrobacterium tumefaciens virE operon encodes a single-stranded DNA-binding protein. Proc Natl Acad Sci USA 85: 2909-2913
40. De Block M, Debrouwer D (1991) Two T-DNA's co-transformed into Brassica napus by a double Agrobacterium tumefaciens infection are mainly integrated at the same locus. Theor Appl Genet 82: 257-263
41. De Buck S, Jacobs A, Van Montagu M, Depicker A (1999) The DNA sequences of T-DNA junctions suggest that complex T-DNA loci are formed by a recombination process resembling T-DNA integration. Plant J 20: 295-304
42. De Cleene M, De Ley J (1976) The host range of crown gall. Bot Rev 42: 389-466
43. de Groot MJ, Bundock P, Hooykaas PJJ, Beijersbergen AG (1998) Agrobacterium tumefaciens-mediated transformation of filamentous fungi. Nat Biotechnol 16: 839-842
44. de Iannino NI, Ugalde RA (1989) Biochemical characterization of avirulent Agrobacterium tumefaciens chvA mutants: synthesis and excretion of P-(1-2)glucan. J Bacteriol 171: 2842-2849
45. De Neve M, De Buck S, Jacobs A, Van Montagu M, Depicker A (1997) T-DNA integration patterns in co-transformed plant cells suggest that T-DNA repeats originate from co-integration of separate T-DNAs. Plant J 11: 15-29
46. Deasey MC, Matthysse AG (1984) Interactions of wild-type and a cellulose-minus mutant of Agrobacterium tumefaciens with tobacco mesophyll and tobacco tissue culture cells. Phytopathol 74: 991-994
47. Deng W, Chen L, Wood DW, Metcalfe T, Liang X, Gordon MP, Comai L, Nester EW (1998) Agrobacterium VirD2 protein interacts with plant host cyclophil-ins. Proc Natl Acad Sci USA 95: 7040-7045
48. Desfeux C, Clough SJ, Bent AF (2000) Female reproductive tissues are the primary target of Agrobacterium-mediated transformation by the Arabidopsis floral-dip method. Plant Physiol 123: 895-904
49. Ditt RF, Nester E, Comai L (2005) The plant cell defense and Agrobacterium tumefaciens. FEMS Microbiol Lett 247: 207-213
50. Ditt RF, Nester EW, Comai L (2001) Plant gene expression response to Agrobacterium tumefaciens. Proc Natl Acad Sci USA: 10954-10959
51. Dommisse EM, Leung DWM, Shaw ML, Conner AJ (1990) Onion is a monocoty-ledonous host for Agrobacterium. Plant Sci 69: 249-257
52. Dong J, Teng W, Buchholz WG, Hall TC (1996) Agrobacterium-mediated transformation of Javanica rice. Mol Breed 2: 267-276
53. Douglas CJ, Halperin W, Nester EW (1982) Agrobacterium tumefaciens mutants affected in attachment to plant cell. J Bacteriol 152: 1265-1275
54. Douglas CJ, Staneloni RJ, Rubin RA, Nester EW (1985) Identification and genetic analysis of an Agrobacterium tumefaciens chromsomal virulence region. J Bacteriol 161: 850-860
55. Duckely M, Hohn B (2003) The VirE2 protein of Agrobacterium tumefaciens: the Yin and Yang of T-DNA transfer. FEMS Microbiol Lett 223: 1-6
56. Duckely M, Oomen C, Axthelm F, Van Gelder P, Waksman G, Engel A (2005) The VirE1VirE2 complex of Agrobacterium tumefaciens interacts with single-stranded DNA and forms channels. Mol Microbiol 58: 1130-1142
57. Dumas F, Duckely M, Pelczar P, Van Gelder P, Hohn B (2001) An Agrobacte-rium VirE2 channel for transferred-DNA transport into plant cells. Proc Natl Acad Sci USA 98: 485-490
58. Durrenberger F, Crameri A, Hohn B, Koukolikova-Nicola Z (1989) Covalently bound VirD2 protein of Agrobacterium tumefaciens protects the T-DNA from exonucleolytic degradation. Proc Natl Acad Sci USA 86: 9154-9158
59. Eisenbrandt R, Kalkum M, Lai EM, Lurz R, Kado CI, Lanka E (1999) Conjuga-tive pili of IncP plasmids, and the Ti plasmid T pilus are composed of cyclic subunits. J Biol Chem 274: 22548-22555
60. Escudero J, Hohn B (1997) Transfer and integration of T-DNA without cell injury in the host plant. Plant Cell 9: 2135-2142
61. Friesner J, Britt AB (2003) Ku80-and DNA ligase IV-deficient plants are sensitive to ionizing radiation and defective in T-DNA integration. Plant J 34: 427-440
62. Fullner KJ, Lara JC, Nester EW (1996) Pilus assembly by Agrobacterium T-DNA transfer genes. Science 273: 1107-1109
63. Gallego ME, Bleuyard JY, Daoudal-Cotterell S, Jallut N, White CI (2003) Ku80 plays a role in non-homologous recombination but is not required for T-DNA integration in Arabidopsis. Plant J 35: 557-565
64. Gaspar YM, Nam J, Schultz CJ, Lee LY, Gilson PR, Gelvin SB, Bacic A (2004) Characterization of the Arabidopsis lysine-rich arabinogalactan-protein AtAGP17 mutant (rat1) that results in a decreased efficiency of Agrobacte-rium transformation. Plant Physiol 135: 2162-2171
65. Geier T, Sangwan RS (1996) Histology and chimeral segreation reveal cell-specific differences in the competence for shoot regeneration and Agrobace-rium-mediated transformation in Kohleria internode explants. Plant Cell Rep 15: 386-390
66. Gelvin SB (1998) Agrobacterium VirE2 proteins can form a complex with T strands in the plant cytoplasm. J Bacteriol 180: 4300-4302
67. Gelvin SB (2000) Agrobacterium and plant genes involved in T-DNA transfer and integration. Annu Rev Plant Physiol Plant Mol Biol 51: 223-256
68. Gelvin SB (2003) Agrobacterium-mediated plant transformation: the biology behind the "gene-jockeying" tool. Microbiol Mol Biol Rev 67: 16-37
69. Gheysen G, Villarroel R, Van Montagu M (1991) Illegitimate recombination in plants: a model for T-DNA integration. Genes Dev 5: 287-297
70. Gietl C, Koukolikova-Nicola Z, Hohn B (1987) Mobilization of T-DNA from Agrobacterium to plant cells involves a protein that binds single-stranded DNA. Proc Natl Acad Sci USA 84: 9006-9010
71. Gouin E, Welch MD, Cossart P (2005) Actin-based motility of intracellular pathogens. Curr Opin Microbiol 8: 35-45
72. Grevelding C, Fantes V, Kemper E, Schell J, Masterson R (1993) Single-copy T-DNA insertions in Arabidopsis are the predominant form of integration in root-derived transgenics, whereas multiple insertions are found in leaf discs. Plant Mol Biol 23: 847-860
73. Guralnick B, Thomsen G, Citovsky V (1996) Transport of DNA into the nuclei of Xenopus oocytes by a modified VirE2 protein of Agrobacterium. Plant Cell 8: 363-373
74. Gurlitz RHG, Lamb PW, Matthysse AG (1987) Involvement of carrot cell surface proteins in attachment of Agrobacterium tumefaciens. Plant Physiol 83: 564-568
75. Hawes MC, Pueppke SG (1989) Variation in binding and virulence of Agrobacte-rium tumefaciens chromosomal virulence (chv) mutant bacteria on different plant species. Plant Physiol 91: 113-118
76. Herrera-Estrella A, Chen Z-M, Van Montagu M, Wang K (1988) VirD proteins of Agrobacterium tumefaciens are required for the formation of a covalent DNA protein complex at the 5' terminus of T-strand molecules. EMBO J 7: 4055-4062
77. Herrera-Estrella A, Van Montagu M, Wang K (1990) A bacterial peptide acting as a plant nuclear targeting signal: the amino-terminal portion of Agrobacterium VirD2 protein directs a beta-galactosidase fusion protein into tobacco nuclei. Proc Natl Acad Sci USA 87: 9534-9537
78. Hiei Y, Ohta S, Komari T, Kumashiro T (1994) Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA. Plant J 6: 271-282
79. Hood EE, Fraley RT, Chilton M-D (1987) Virulence of Agrobacterium tumefa-ciens strain A281 on legumes. Plant Physiol 83: 529-534
80. Howard EA, Citovsky V (1990) The emerging structure of the Agrobacterium T-DNA transfer complex. BioEssays 12: 103-108
81. Howard EA, Winsor BA, De Vos G, Zambryski P (1989) Activation of the T-DNA transfer process in Agrobacterium results in the generation of a T-strand protein complex: tight association of VirD2 with the 5' ends of T-strands. Proc Natl Acad Sci USA 86: 4017-4021
82. Howard EA, Zupan JR, Citovsky V, Zambryski P (1992) The VirD2 protein of A. tumefaciens contains a C-terminal bipartite nuclear localization signal: implications for nuclear uptake of DNA in plant cells. Cell 68: 109-118
83. Hwang HH, Gelvin SB (2004) Plant proteins that interact with VirB2, the Agro-bacterium tumefaciens pilin protein, mediate plant transformation. Plant Cell 16: 3148-3167
84. Ishida Y, Saito H, Ohta S, Hiei Y, Komari T, Kumashiro T (1996) High efficiency transformation of maize (Zea mays L.) mediated by Agrobacterium tumefa-ciens. Nat Biotechnol 14: 745-750
85. Jakubowski SJ, Cascales E, Krishnamoorthy V, Christie PJ (2005) Agrobacterium tumefaciens VirB9, an outer-membrane-associated component of a type IV secretion system, regulates substrate selection and T-pilus biogenesis. J Bacte-riol 187: 3486-3495
86. Kado CI (1994) Promiscuous DNA transfer system of Agrobacterium tumefa-ciens: role of the virB operon in sex pilus assembly and synthesis. Mol Mi-crobiol 12: 17-22
87. Kamoun S, Cooley MB, Rogowsky PM, Kado CI (1989) Two chromosomal loci involved in production of exopolysaccharide in Agrobacterium tumefaciens. J Bacteriol 171: 1755-1759
88. Kirik A, Salomon S, Puchta H (2000) Species-specific double-strand break repair and genome evolution in plants. Embo J 19: 5562-5566
89. Koukolikova-Nicola Z, Hohn B (1993) How does the T-DNA of Agrobacterium tumefaciens find its way into the plant cell nucleus? Biochimie 75: 635-638
90. Koukolikova-Nicola Z, Raineri D, Stephens K, Ramos C, Tinland B, Nester EW, Hohn B (1993) Genetic analysis of the virD operon of Agrobacterium tumefa-ciens: a search for functions involved in transport of T-DNA into the plant cell nucleus and in T-DNA integration. J Bacteriol 175: 723-731
91. Kunik T, Tzfira T, Kapulnik Y, Gafni Y, Dingwall C, Citovsky V (2001) Genetic transformation of HeLa cells by Agrobacterium. Proc Natl Acad Sci USA 98: 1871-1876
92. Lacroix B, Tzfira T, Vainstein A, Citovsky V (2006) A case of promiscuity: Agrobacterium's endless hunt for new partners. Trends Genet 22: 29-37
93. Lacroix B, Vaidya M, Tzfira T, Citovsky V (2005) The VirE3 protein of Agrobac-terium mimics a host cell function required for plant genetic transformation. EMBO J 24: 428-437
94. Lai EM, Kado CI (1998) Processed VirB2 is the major subunit of the promiscuous pilus of Agrobacterium tumefaciens. J Bacteriol 180: 2711-2717
95. Lee LY, Gelvin SB, Kado CI (1999) pSa causes oncogenic suppression of Agro-bacterium by inhibiting VirE2 protein export. J Bacteriol 181: 186-196
96. Levee V, Garin E, Klimaszewska K, Seguin A (1999) Stable genetic transformation of white pine (Pinus strobus L.) after cocultivation of embryogenic tisues with Agrobacterium tumefaciens. Mol Breed 5: 429-440
97. Li J, Krichevsky A, Vaidya M, Tzfira T, Citovsky V (2005a) Uncoupling of the functions of the Arabidopsis VIP1 protein in transient and stable plant genetic transformation by Agrobacterium. Proc Natl Acad Sci USA 102: 5733-5738
98. Li J, Vaidya M, White C, Vainstein A, Citovsky V, Tzfira T (2005b) Involvement of KU80 in T-DNA integration in plant cells. Proc Natl Acad Sci USA 102: 19231-19236
99. Loyter A, Rosenbluh J, Zakai N, Li J, Kozlovsky SV, Tzfira T, Citovsky V (2005) The plant VirE2 interacting protein 1. A molecular link between the Agrobac-terium T-complex and the host cell chromatin? Plant Physiol 138: 1318-1321
100. Lu H-J, Zhou X-R, Gong Z-X, Upadhyaya NM (2001) Generation of selectable marker-free transgenic rice using double right-border (DRB) vectors. Aust J Plant Physiol 28: 241-248
101. Macara IG (2001). Transport into and out of the nucleus. Microbiol Mol Biol Rev 65: 570-594
102. Matthysse AG (1987) Characterization of nonattaching mutants of Agrobacterium tumefaciens. J Bacteriol 169: 313-323
103. Matthysse AG, Holmes KV, Gurlitz RH (1981) Elaboration of cellulose fibrils by Agrobacterium tumefaciens during attachment to carrot cells. J Bacteriol 145: 583-595
104. Matthysse AG, Marry M, Krall L, Kaye M, Ramey BE, Fuqua C, White AR (2005) The effect of cellulose overproduction on binding and biofilm formation on roots by Agrobacterium tumefaciens. Mol Plant Microbe Interact 18: 1002-1010
105. Mauro AO, Pfeiffer TW, Collins GB (1995) Inheritance of soybean susceptibility to Agrobacterium tumefaciens and its relationship to transformation. Crop Sci 35: 1152-1156
106. Mayerhofer R, Koncz-Kalman Z, Nawrath C, Bakkeren G, Crameri A, Angelis K, Redei GP, Schell J, Hohn B, Koncz C (1991) T-DNA integration: a mode of illegitimate recombination in plants. EMBO J 10: 697-704
107. McAfee BJ, White EE, Pelcher LE, Lapp MS (1993) Root induction in pine (Pinus) and larch (Larix) spp. using Agrobacterium rhizogenes. Plant Cell Tissue Organ Cultur 34: 53-62
108. Melchers LS, Maroney MJ, den Dulk-Ras A, Thompson DV, van Vuuren HAJ, Schilperoort RA, Hooykaas PJJ (1990) Octopine and nopaline strains of Agrobacterium tumefaciens differ in virulence; molecular characterization of the virF locus. Plant Mol Biol 14: 249-259
109. Merkle T (2004) Nucleo-cytoplasmic partitioning of proteins in plants: Implications for the regulation of environmental and developmental signaling. Curr Genet 44: 231-260
110. Michielse CB, Ram AFJ, Hooykaas PJJ, van den Hondel CAMJJ (2004) Agrobac-terium-mediated transformation of Aspergillus awamori in the absence of full-length VirD2, VirC2, or VirE2 leads to insertion of aberrant T-DNA structures. J Bacteriol 186: 2038-2045
111. Morris JW, Morris RO (1990) Identification of an Agrobacterium tumefaciens virulence gene inducer from the pinaceous gymnosperm Pseudotsuga menzie-sii. Proc Natl Acad Sci USA 87: 3614-3618
112. Mysore KS, Bassuner B, Deng X-B, Darbinian NS, Motchoulski A, Ream W, Gelvin SB (1998) Role of the Agrobacterium tumefaciens VirD2 protein in T-DNA transfer and integration. Mol Plant-Microbe Interact 11: 668-683
113. Mysore KS, Kumar CTR, Gelvin SB (2000a) Arabidopsis ecotypes and mutants that are recalcitrant to Agrobacterium root transformation are susceptible to germ-line transformation. Plant J 21: 9-16
114. Mysore KS, Nam J, Gelvin SB (2000b) An Arabidopsis histone H2A mutant is deficient in Agrobacterium T-DNA integration. Proc Natl Acad Sci USA 97: 948-953
115. Nam J, Matthysse AG, Gelvin SB (1997) Differences in susceptibility of Arabi-dopsis ecotypes to crown gall disease may result from a deficiency in T-DNA integration. Plant Cell 9: 317-333
116. Nam J, Mysore KS, Gelvin SB (1998) Agrobacterium tumefaciens transformation of the radiation hypersensitive Arabidopsis thaliana mutants uvh1 and rad5. Mol Plant-Microbe Interact 11: 1136-1141
117. Nam J, Mysore KS, Zheng C, Knue MK, Matthysse AG, Gelvin SB (1999) Identification of T-DNA tagged Arabidopsis mutants that are resistant to transformation by Agrobacterium. Mol Gen Genet 261: 429-438
118. Narasimhulu SB, Deng X-B, Sarria R, Gelvin SB (1996) Early transcription of Agrobacterium T-DNA genes in tobacco and maize. Plant Cell 8: 873-886
119. Neff NT, Binns AN (1985) Agrobacterium tumefaciens interaction with suspension-cultured tomato cells. Plant Physiol 77: 35-42
120. O'Connell KP, Handelsman J (1999) chvA locus may be involved in export of neutral cyclic beta-1, 2 linked D-glucan from Agrobacterium tumefaciens. Mol Plant Microbe Interact 2: 11-16
121. Otten L, DeGreve H, Leemans J, Hain R, Hooykass P, Schell J (1984) Restoration of virulence of vir region mutants of Agrobacterium tumefaciens strain B6S3 by coinfection with normal and mutant Agrobacterium strains. Mol Gen Genet 195: 159-163
122. Owens LD, Cress DE (1984) Genotypic variability of soybean response to Agro-bacterium strains harboring the Ti or Ri plasmids. Plant Physiol 77: 87-94
123. Pena L, Seguin A (2001) Recent advances in the genetic transformation of trees. Trends Biotechnol 19: 500-506
124. Piers KL, Heath JD, Liang X, Stephens KM, Nester EW (1996) Agrobacterium tumefaciens-mediated transformation of yeast. Proc Natl Acad Sci USA 93: 1613-1618
125. Puvanesarajah V, Schell FM, Stacey G, Douglas CJ, Nester EW (1985) Role for 2-linked-p-D-glucan in the virulence of Agrobacterium tumefaciens. J Bacte-riol 164: 102-106
126. Ramey BE, Matthysse AG, Fuqua C (2004) The FNR-type transcriptional regulator SinR controls maturation of Agrobacterium tumefaciens biofilms. Mol Microbiol 52: 1495-1511
127. Rao P (2002) Involvement of the plant cytoskeleton in Agrobacterium transformation. PhD Thesis, Purdue University
128. Rashid H, Yokoi S, Toriyama K, Hinata K (1996) Transgenic plant production mediated by Agrobacterium in Indica rice. Plant Cell Rep 15: 727-730
129. Regensburg-Tuink AJ, Hooykaas PJJ (1993) Transgenic N. glauca plants expressing bacterial virulence gene virF are converted into hosts for nopaline strains of A tumefaciens. Nature 363: 69-71
130. Relic B, Andjelkovic M, Rossi L, Nagamine Y, Hohn B (1998) Interaction of the DNA modifying proteins VirD1 and VirD2 of Agrobacterium tumefaciens: analysis by subcellular localization in mammalian cells. Proc Natl Acad Sci USA 95: 9105-9110
131. Reuhs BL, Kim JS, Matthysse AG (1997) Attachment of Agrobacterium tumefaciens to carrot cells and Arabidopsis wound sites is correlated with the presence of a cell-associated, acidic polysaccharide. J Bacteriol 179: 5372-5379
132. Rhee Y, Gurel F, Gafni Y, Dingwall C, Citovsky V (2000) A genetic system for detection of protein nuclear import and export. Nat Biotechnol 18: 433-437
133. Robbs SL, Hawes MC, Lin H-J, Pueppke SG, Smith LY (1991) Inheritance of resistance to crown gall in Pisum sativum. Plant Physiol 95: 52-57
134. Robertson JL, Holliday T, Matthysse AG (1988) Mapping of Agrobacterium tumefaciens chromosomal genes affecting cellulose synthesis and bacterial attachment to host cells. J Bacteriol 170: 1408-1411
135. Rossi L, Hohn B, Tinland B (1993) The VirD2 protein of Agrobacterium tumefaciens carries nuclear localization signals important for transfer of T-DNA to plant. Mol Gen Genet 239: 345-353
136. Sagulenko V, Sagulenko E, Jakubowski S, Spudich E, Christie PJ (2001) VirB7 lipoprotein is exocellular and associates with the Agrobacterium tumefaciens T pilus. J Bacteriol 183: 3642-3651
137. Sallaud C, Gay C, Larmande P, Bes M, Piffanelli P, Piegu B, Droc G, Regad F, Bourgeois E, Meynard D, Perin C, Sabau X, Ghesquiere A, Glaszmann JC, Delseny M, Guiderdoni E (2004) High throughput T-DNA insertion mutagenesis in rice: a first step towards in silico reverse genetics. Plant J 39: 450-464
138. Salman H, Abu-Arish A, Oliel S, Loyter A, Klafter J, Granek R, Elbaum M (2005) Nuclear localization signal peptides induce molecular delivery along micro-tubules. Biophys J 89: 2134-2145
139. Salomon S, Puchta H (1998) Capture of genomic and T-DNA sequences during double-strand break repair in somatic plant cells. EMBO J 17: 6086-6095
140. Sangwan RS, Bourgeois Y, Brown S, Vasseur G, Sangwan-Norreel B (1992) Characterization of competent cells and early events of Agrobacterium-mediated genetic transformation in Arabidopsis thaliana. Planta 188: 439-456
141. Schafer W, Gorz A, Kahl G (1987) T-DNA integration and expression in a mono-cot crop plant after induction of Agrobacterium. Nature 327: 529-532
142. Schmidt-Eisenlohr H, Domke N, Angerer C, Wanner G, Zambryski P, Baron C (1999) Vir proteins stabilize VirB5 and mediate its association with the T pilus of Agrobacterium tumefaciens. J Bacteriol 181: 7485-7492
143. Schrammeijer B, den Dulk-Ras A, Vergunst AC, Jácome JE, Hooykaas PJJ (2003) Analysis of Vir protein translocation from Agrobacterium tumefaciens using Saccharomyces cerevisiae as a model: evidence for transport of a novel effector protein VirE3. Nucleic Acids Res 31: 860-868
144. Schrammeijer B, Risseeuw E, Pansegrau W, Regensburg-Tuïnk TJG, Crosby WL, Hooykaas PJJ (2001) Interaction of the virulence protein VirF of Agrobacte-rium tumefaciens with plant homologs of the yeast Skp1 protein. Curr Biol 11: 258-262
145. Sen P, Pazour GJ, Anderson D, Das A (1989) Cooperative binding of Agrobacte-rium tumefaciens VirE2 protein to single-stranded DNA. J Bacteriol 171: 2573-2580
146. Shibasaki F, Kondo E, Akagi T, McKeon F (1997) Suppression of signaling through transcription factor NF-AT by interaction between calcineurin and Bcl-2. Nature 386: 728-731
147. Shibasaki F, Price ER, Milan D, McKeon F (1996) Role of kinases and the phos-phatase calcineurin in the nuclear shuttling of transcription factor NF-AT4. Nature 382: 370-373
148. Silva H, Yoshioka K, Dooner HK, Klessig DF (1999) Characterization of a new Arabidopsis mutant exhibiting enhanced disease resistance. Mol Plant-Microbe Interact 12: 1053-1063
149. Smarrelli J, Watters MT, Diba LH (1986) Response of various cucurbits to infection by plasmid-harboring strains of Agrobacterium. Plant Physiol 82: 622-624
150. Smith HMS, Raikhel NV (1998) Nuclear localization signal receptor importin alpha associates with the cytoskeleton. Plant Cell 10: 1791-1799
151. Sparrow PAC, Townsend TM, Arthur AE, Dale PJ, Irwin JA (2004) Genetic analysis of Agrobacterium tumefaciens susceptibility in Brassica oleracea. Theor Appl Genet 108: 644-650
152. Stomp A-M, Loopstra C, Chilton WS, Sederoff RR, Moore LW (1990) Extended host range of Agrobacterium tumefaciens in the genus Pinus. Plant Physiol 92: 1226-1232.
153. Swart S, Logman TJ, Smit G, Lugtenberg BJJ, Kijne JW (1994) Purification and partial characterization of a glycoprotein from pea (Pisum sativum) with receptor activity for rhicadhesin, an attachment protein of Rhizobiaceae. Plant Mol Biol 24: 171-183
154. Szegedi E, Kozma P (1984) Studies on the inheritance of resistance to crown gall disease of grapevine. Vitis 23: 121-126
155. Tao Y (1998) Isolation and characterization of a cDNA encoding a plant phos-phatase implicated in nuclear import of the Agrobacterium VirD2 protein. PhD Thesis, Purdue University
156. Tao Y, Rao PK, Bhattacharjee S, Gelvin SB (2004) Expression of plant protein phosphatase 2C interferes with nuclear import of the Agrobacterium T-complex protein VirD2. Proc Natl Acad Sci USA 101: 5164-5169
157. Thomashow MF, Karlinsey JE, Marks JR, Hurlbert RE (1987) Identification of a new virulence locus in Agrobacterium tumefaciens that affects polysaccharide composition and plant cell attachment. J Bacteriol 169: 3209-3216
158. Tian L, Wang J, Fong MP, Chen M, Cao H, Gelvin SB, Chen ZJ (2003) Genetic control of developmental changes induced by disruption of Arabidopsis his-tone deacetylase 1 (AtHD1) expression. Genetics 165: 399-409
159. Tingay S, McElroy D, Kalla R, Fieg S, Wang M, Thornton S, Brettell R (1997) Agrobacterium tumefaciens-mediated barley transformation. Plant J 11: 1369-1376
160. Tinland B (1996) The integration of T-DNA into plant genomes. Trends Plant Sci 1: 178-184
161. Tinland B, Hohn B (1995) Recombination between prokaryotic and eukaryotic DNA: integration of Agrobacterium tumefaciens T-DNA into the plant genome. In JK Settoe, ed, Genetic Engineering, Plenum Press, New York pp. 209-229
162. Tinland B, Koukolikova-Nicola Z, Hall MN, Hohn B (1992) The T-DNA-linked VirD2 protein contains two distinct nuclear localization signals. Proc Natl Acad Sci USA 89: 7442-7446
163. Toki S (1997) Rapid and efficient Agrobacterium-mediated transformation in rice. Plant Mol Biol Rep 15: 16-21
164. Tzfira T, Citovsky V (2002) Partners-in-infection: host proteins involved in the transformation of plant cells by Agrobacterium. Trends Cell Biol 12: 121-129
165. Tzfira T, Citovsky V (2003) The Agrobacterium-plant cell interaction. Taking biology lessons from a bug. Plant Physiol 133: 943-947
166. Tzfira T, Frankman LR, Vaidya M, Citovsky V (2003) Site-specific integration of Agrobacterium tumefaciens T-DNA via double-stranded intermediates. Plant Physiol 133: 1011-1023
167. Tzfira T, Li J, Lacroix B, Citovsky V (2004a) Agrobacterium T-DNA integration: molecules and models. Trends Genet 20: 375-383
168. Tzfira T, Rhee Y, Chen M-H, Kunik T, Citovsky V (2000) Nucleic acid transport in plant-microbe interactions: the molecules that walk through the walls. Annu Rev Microbiol 54: 187-219
169. Tzfira T, Vaidya M, Citovsky V (2001) VIP1, an Arabidopsis protein that interacts with Agrobacterium VirE2, is involved in VirE2 nuclear import and Agrobacterium infectivity. EMBO J 20: 3596-3607
170. Tzfira T, Vaidya M, Citovsky V (2002) Increasing plant susceptibility to Agrobac-terium infection by overexpression of the Arabidopsis nuclear protein VIP1. Proc Natl Acad Sci USA 99: 10435-10440
171. Tzfira T, Vaidya M, Citovsky V (2004b) Involvement of targeted proteolysis in plant genetic transformation by Agrobacterium. Nature 431: 87-92
172. Valentine L (2003) Agrobacterium tumefaciens and the plant: the David and Goliath of modern genetics. Plant Physiol 133: 948-955
173. van Attikum H, Bundock P, Hooykaas PJJ (2001) Non-homologous end-joining proteins are required for Agrobacterium T-DNA integration. Embo J 20: 6550-6558
174. van Attikum H, Bundock P, Overmeer RM, Lee LY, Gelvin SB, Hooykaas PJJ (2003) The Arabidopsis AtLIG4 gene is required for the repair of DNA damage, but not for the integration of Agrobacterium T-DNA. Nucleic Acids Res 31: 4247-4255
175. van Attikum H, Hooykaas PJJ (2003) Genetic requirements for the targeted integration of Agrobacterium T-DNA in Saccharomyces cerevisiae. Nucleic Acids Res 31: 826-832
176. Vandromme M, Gauthier-Rouviere C, Lamb N, Fernandez A (1996) Regulation of transcription factor localization: Fine-tuning of gene expression. Trends Bio-chem Sci 21: 59-64
177. van Wordragen MF, Dons HJM (1992) Agrobacterium tumefaciens-mediated transformation of recalcitrant crops. Plant Mol Biol Rep 10: 12-36
178. Veena, Jiang H, Doerge RW, Gelvin SB (2003) Transfer of T-DNA and Vir proteins to plant cells by Agrobacterium tumefaciens induces expression of host genes involved in mediating transformation and suppresses host defense gene expression. Plant J 35: 219-236
179. Vogel AM, Das A (1992) Mutational analysis of Agrobacterium tumefaciens virD2: tyrosine 29 is essential for endonuclease activity. J Bacteriol 174: 303-308
180. Wagner VT, Matthysse AG (1992) Involvement of vitronectin-like protein in attachment of Agrobacterium tumefaciens to carrot suspension culture cells. J Bacteriol 174: 5999-6003
181. Ward ER, Barnes WM (1988) VirD2 protein of Agrobacterium tumefaciens very
182. Wenck AR, Quinn M, Whetten RW, Pullman G, Sederoff R (1999) High-efficiency Agrobacterium-mediated transformation of Norway spruce (Picea abies) and loblolly pine (Pinus taeda). Plant Mol Biol 39: 407-416
183. Windels P, De Buck S, Van Bockstaele E, De Loose M, Depicker A (2003) T-DNA integration in Arabidopsis chromosomes. Presence and origin of filler DNA sequences. Plant Physiol 133: 2061-2068
184. Ye GN, Stone D, Pang SZ, Creely W, Gonzalez K, Hinchee M (1999) Arabidopsis ovule is the target for Agrobacterium in planta vacuum infiltration transformation. Plant J 19: 249-257
185. Yi H, Mysore KS, Gelvin SB (2002) Expression of the Arabidopsis histone H2A-1 gene correlates with susceptibility to Agrobacterium transformation. Plant J 32: 285-298
186. Yi H, Sardesai N, Fujinuma T, Chan C-W, Veena, Gelvin SB (2006) Constitutive expression exposes functional redundancy between the Arabidopsis histone H2A gene HTA1 and other H2A gene family members. Plant Cell 18: 1575-1589
187. Young C, Nester EW (1988) Association of the VirD2 protein with the 5' end of T-strands in Agrobacterium tumefaciens. J Bacteriol 170: 3367-3374
188. Zhu Y, Nam J, Carpita NC, Matthysse AG, Gelvin SB (2003a) Agrobacterium-mediated root transformation is inhibited by mutation of an Arabidopsis cellulose synthase-like gene. Plant Physiol 133: 1000-1010
189. Zhu Y, Nam J, Humara JM, Mysore KS, Lee LY, Cao H, Valentine L, Li J, Kaiser AD, Kopecky AL, Hwang HH, Bhattacharjee S, Rao PK, Tzfira T, Rajagopal J, Yi H, Veena, Yadav BS, Crane YM, Lin K, Larcher Y, Gelvin MJ, Knue M, Ramos C, Zhao X, Davis SJ, Kim SI, Ranjith-Kumar CT, Choi YJ, Hallan VK, Chattopadhyay S, Sui X, Ziemienowicz A, Matthysse AG, Citovsky V, Hohn B, Gelvin SB (2003b) Identification of Arabidopsis rat mutants. Plant Physiol 132: 494-505
190. Ziemienowicz A, Görlich D, Lanka E, Hohn B, Rossi L (1999) Import of DNA into mammalian nuclei by proteins originating from a plant pathogenic bacterium. Proc Natl Acad Sci USA 96: 3729-3733
191. Ziemienowicz A, Merkle T, Schoumacher F, Hohn B, Rossi L (2001) Import of Agrobacterium T-DNA into plant nuclei: Two distinct functions of VirD2 and VirE2 proteins. Plant Cell 13: 369-383
192. Zorreguieta A, Ugalde RA (1986) Formation in Rhizobium and Agrobacterium spp. of a 235-kilodalton protein intermediate in b-D(1-2) glucan synthesis. J Bacteriol 167: 947-951
193. Zupan J, Citovsky V, Zambryski PC (1996) Agrobacterium VirE2 protein mediates nuclear uptake of single-stranded DNA in plant cells. Proc Natl Acad Sci USA 93: 2392-2397
194. Zupan J, Muth TR, Draper O, Zambryski PC (2000) The transfer of DNA from Agrobacterium tumefaciens into plants: a feast of fundamental insights. Plant J 23: 11-28