Vir proteins stabilize VirB5 and mediate its association with the T pilus of Agrobacterium tumefaciens

Journal of Bacteriology

Volumn 181, Issue 24, 1999, Pages 7485-7492

  Schmidt Eisenlohr, Heike ^a   Domke, Natalie ^a   Angerer, Christina ^a   Wanner, Gerhard ^b   Zambryski, Patricia C ^c   Baron, Christian ^a  

^a Institut fur Genetik und Mikrobiologie   (Germany)

^b UNIVERSITY OF MUNICH   (Germany)

^c UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BACTERIAL VIRULENCE; CONTROLLED STUDY; FLAGELLUM; GENE DELETION; GENE INDUCTION; NONHUMAN; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN LOCALIZATION; PROTEIN PROTEIN INTERACTION; PROTEIN STABILITY; RHIZOBIUM RADIOBACTER;

AGROBACTERIUM TUMEFACIENS;

EID: 0032762640     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.181.24.7485-7492.1999     Document Type: Article

References (49)

1. Anderson, L. B., A. Vogel Hertzel, and A. Das. 1996. Agrobacterium tumefaciens VirB7 and VirB9 form a disulfide-linked protein complex. Proc. Natl. Acad. Sci. USA 93:8889-8894.
2. Anthony, K. G., C. Sherbourne, R. Sherburne, and L. S. Frost. 1994. The role of the pilus in recipient cell recognition during bacterial conjugation mediated by F-like plasmids. Mol. Microbiol. 13:939-953.
3. Baron, C., M. Llosa, S. Zhou, and P. C. Zambryski. 1997. VirB1, a component of the T-complex transfer machinery of Agrobacterium tumefaciens, is processed to a C-terminal secreted product, VirB1*. J. Bacteriol. 179:1203-1210.
4. Baron, C., Y. R. Thorstenson, and P. C. Zambryski. 1997. Biochemical analysis of the complex between the lipoprotein VirB7 and VirB9 in the membranes of Agrobacterium tumefaciens. J. Bacteriol. 179:1211-1218.
5. Baron, C., and P. C. Zambryski. 1996. Plant transformation: a pilus in Agrobacterium T-DNA transfer. Curr. Biol. 6:1567-1569.
6. Bayer, M., R. Eferl, G. Zellnig, K. Terferle, A. Dijkstra, G. Koraimann, and G. Högenauer. 1995. Gene 19 of plasmid R1 is required for both efficient conjugative DNA transfer and bacteriophage R17 infection. J. Bacteriol. 177:4279-4288.
7. Berger, B. R., and P. J. Christie. 1994. Genetic complementation analysis of the Agrobacterium tumefaciens virB operon: virB2 through virB11 are essential virulence genes. J. Bacteriol. 176:3646-3660.
8. Christie, P. J. 1997. Agrobacterium tumefaciens T-complex transport apparatus: a paradigm for a new family of multifunctional transporters in eubacteria. J. Bacteriol. 179:3085-3094.
9. Chumakov, M. I., and I. V. Kurbanova. 1998. Localization of the protein VirB1 involved in contact formation during conjugation among Agrobacterium cells. FEMS Microbiol. Lett. 168:297-301.
10. Depicker, A., M. DeWilde, G. De Vos, R. De Vos, M. Van Montagu, and J. Schell. 1980. Molecular cloning of overlapping segments of the nopaline Ti plasmid of pTiC58 as a means to restriction endonuclease mapping. Plasmid 31:193-211.
11. Dürrenberger, M. B., W. Villiger, and T. Bächi. 1991. Conjugational junctions: morphology of specific contacts in conjugating Escherichia coli bacteria. J. Struct. Biol. 107:146-156.
12. Eisenbrandt, R., M. Kalkum, E. M. Lai, R. Lurz, C. I. Kado, and E. Lanka. 1999. Conjugative pili of IncP plasmids and the Ti plasmid T pilus are composed of cyclic subunits. J. Biol Chem. 274:22548-22555.
13. Frost, L. S., J. Lee, D. Scraba, and W. Paranchych. 1986. Two monoclonal antibodies specific for different epitopes within the amino-terminal region of F pilin. J. Bacteriol. 168:192-198.
14. Frost, L. S., K. Ippen-Ihler, and R. A. Skurray. 1994. Analysis of the sequence and gene products of the transfer region of the F sex factor. Microbiol. Rev. 58:162-210.
15. Fullner, K. J., J. L. Lara, and E. W. Nester. 1996. Pilus assembly by Agrobacterium T-DNA transfer genes. Science 273:1107-1109.
16. Fullner, K. J., and E. W. Nester. 1996. Temperature affects the T-DNA transfer machinery of Agrobacterium tumefaciens. J. Bacteriol. 178:1498-1504.
17. Haase, J., and E. Lanka. 1997. A specific protease encoded by the conjugative DNA transfer systems of IncP and Ti plasmids is essential for pilus synthesis. J. Bacteriol. 179:5728-5735.
18. Harlow, E., and D. Lane (ed.). 1988. Antibodies: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
19. Hooykaas, P. J. J., and A. G. M. Beiersbergen. 1994. The virulence system of Agrobacterium tumefaciens. Annu. Rev. Phytopathol. 32:157-179.
20. Hultgren, S. J., S. Abraham, M. Caparon, P. Falk, J. W. St. Geme III, and S. Normark. 1993. Pilus and nonpilus bacterial adhesins: assembly and function in cell recognition. Cell 73:887-901.
21. Jones, A. L., E.-M. Lai, K. Shirasu, and C. I. Kado. 1996. VirB2 is a processed pili-like protein encoded by the Agrobacterium tumefaciens Ti plasmid. J. Bacteriol. 178:5706-5711.
22. Jones, A. L., K. Shirasu, and C. I. Kado. 1994. The product of the virB4 gene of Agrobacterium tumefaciens promotes accumulation of VirB3 protein. J. Bacteriol. 176:5255-5261.
23. Kado, C. I. 1994. Promiscuous DNA transfer system of Agrobacterium tumefaciens: role of the virB operon in sex pilus assembly and synthesis. Mol. Microbiol. 12:17-22.
24. Kao, J. C., K. L. Perry, and C. I. Kado. 1982. Indoleacetic add complementation and its relation to host range specifying genes on the Ti plasmid of Agrobacterium tumefaciens. Mol. Gen. Genet. 188:425-432.
25. Krall, L., and C. Baron. Unpublished data.
26. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.
27. Lai, E.-M., and C. I. Kado. 1998. Processed VirB2 is the major subunit of the promiscuous pilus of Agrobacterium tumefaciens. J. Bacteriol. 180:2711-2717.
28. Lanka, E., and B. M. Wilkins. 1995. DNA processing reactions in bacterial conjugation. Annu. Rev. Biochem. 64:141-169.
29. Lennherr, H., A.-M. Hansen, and T. Ilyina. 1998. Penetration of the bacterial cell wall: a family of lytic transglycosylases in bacteriophages and conjugative plasmids. Mol. Microbiol. 30:454-457.
30. Lessl, M., D. Balzer, W. Pansegrau, and E. Lanka. 1992. Sequence similarities between the RP4 Tra2 and the Ti VirB region strongly support the conjugation model for T-DNA transfer. J. Biol. Chem. 267:20471-20480.
31. Llosa, M., and P. C. Zambryski. 1998. On the origin and function of pili. Trends Microbiol. 6:98-99.
32. Llosa, M., J. Zupan, and P. C. Zambryski. Unpublished data.
33. Maniatis, T. A., E. F. Fritsch, and J. Sambrook. 1982. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
34. Mushegian, A. R., K. J. Fullner, E. V. Koonin, and E. W. Nester. 1996. A family of lysozyme-like virulence factors in bacterial pathogens. Proc. Natl. Acad. Sci. USA 93:7321-7326.
35. Roine, E., W. Wei, J. Yuan, E. L. Nurmiaho-Lassila, N. Kalkkinen, M. Romantschuk, and S. Y. He. 1997. Hrp pilus: an hrp-dependent bacterial surface appendage produced by Pseudomonas syringae pv. tomato DC3000. Proc. Natl. Acad. Sci. USA 94:3459-3464.
36. Sakellaris, H., D. P. Balding, and J. R. Scott. 1996. Assembly proteins of CS1 pili of enterotoxigenic Escherichia coli. Mol. Microbiol. 21:529-541.
37. Schägger, H., and G. von Jagow. 1987. Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range of 1 to 100 kDa. Anal. Biochem. 166:368-379.
38. Schmidt-Eisenlohr, H., N. Domke, and C. Baron. 1999. TraC of IncN plasmid pKM101 associates with membranes and extracellular high-molecular-weight structures in Escherichia coli. J. Bacteriol. 181:5563-5571.
39. Sheng, J., and V. Citovsky. 1996. Agrobacterium-plant cell DNA transport: have virulence proteins, will travel. Plant Cell 8:1699-1710.
40. Shirasu, K., and C. I. Kado. 1993. Membrane localization of the Ti plasmid VirB proteins involved in the biosynthesis of a pilin-like structure in Agrobacterium tumefaciens. FEMS Microbiol. Lett. 111:287-294.
41. Soto, G. E., and S. J. Hultgren. 1999. Bacterial adhesins: common themes and variations in architecture and assembly. J. Bacteriol. 181:1059-1071.
42. Spudich, G. M., D. Fernandez, X.-R. Zhou, and P. J. Christie. 1996. Intermolecular disulfide bonds stabilize VirB7 homodimers and VirB7/VirB9 heterodimers during biogenesis of the Agrobacterium tumefaciens T-complex transport apparatus. Proc. Natl. Acad. Sci. USA 93:7512-7515.
43. Stachel, S. E., B. Timmermann, and P. C. Zambryski. 1986. Generation of single-stranded T-DNA molecules during the initial stages of T-DNA transfer from Agrobacterium tumefaciens to plant cells. Nature 322:706-712.
44. van Larebeke, N., G. Engler, M. Holsters, S. van den Elsacker, I. Zaenen, R. A. Schilperoort, and J. Schell. 1974. Large plasmids in Agrobacterium tumefaciens essential for crown gall-inducing ability. Nature 252:169-170.
45. Winans, S. C., D. L. Burns, and P. J. Christie. 1996. Adaptation of a conjugal system for the export of pathogenic macromolecules. Trends Microbiol. 4: 64-68.
46. Winans, S. C., and G. C. Walker. 1985. Conjugal transfer system of the N incompatibility plasmid pKM101. J. Bacteriol. 161:402-410.
47. Yanisch-Perron, C., J. Vieira, and J. Messing. 1985. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC18 vectors. Gene 33:103-119.
48. Zambryski, P. C. 1992. Chronicles from the Agrobacterium-plant cell DNA transfer story. Annu. Rev. Plant Physiol. Plant Mol. Biol. 43:465-490.
49. Zupan, J. R., D. Ward, and P. C. Zambryski. 1998. Assembly of the VirB transport complex for DNA transfer from Agrobacterium tumefaciens to plant cells. Curr. Opin. Microbiol. 1:649-655.