Measurement of wheat germ agglutinin binding with a fluorescence microscope

Cytometry Part A

Volumn 75, Issue 10, 2009, Pages 874-881

  Model, Michael A ^a   Reese, Jennifer L ^a   Fraizer, Gail C ^a  

^a KENT STATE UNIVERSITY   (United States)

Author keywords

Calibration; Fluorescence microscopy; MESF; Quantum yield; Shading correction; Standards; Wheat germ agglutinin

Indexed keywords

WHEAT GERM AGGLUTININ;

ACCURACY; ARTICLE; CALIBRATION; CELL TYPE; CONTROLLED STUDY; FEMALE; FLUORESCENCE; FLUORESCENCE MICROSCOPE; HELA CELL; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; MICROSCOPY; MOLECULE; PRIORITY JOURNAL; RELIABILITY; REPRODUCIBILITY;

CALIBRATION; ERYTHROCYTES; FLOW CYTOMETRY; FLUORESCENT DYES; HELA CELLS; HUMANS; MICROSCOPY, FLUORESCENCE; PROTEIN BINDING; RHODAMINES; SOLUBILITY; SUBSTRATE SPECIFICITY; WHEAT GERM AGGLUTININS;

EID: 70349495872     PISSN: 15524922     EISSN: 15524930     Source Type: Journal    
DOI: 10.1002/cyto.a.20787     Document Type: Article

References (45)

1. Schwartz A, Wang L, Early E, Gaigalas A, Zhang Y, Marti GE, Vogt RF. Quantitating fluorescence intensity from fluorophore: The definition of MESF assignment. J Res Natl Inst Stand Technol 2002;107:83-91. (Pubitemid 34600466)
2. Wang L, Gaigalas AK, Abbasi F, Marti GE, Vogt RF, Schwartz A. Quantitating fluorescence intensity from fluorophores: Practical use of MESF values. J Res Natl Inst Stand Technol 2002;107:339-353. (Pubitemid 35361066)
3. Schwartz A, Gaigalas AK, Wang L, Marti GE, Vogt RF, Fernandez-Repollet E. Formalization of the MESF unit of fluorescence intensity. Cytometry Part B 2004;57B:1-6. (Pubitemid 38297617)
4. Gratama JW, D'hautcourt JL, Mandy F, Rothe G, Barnett D, Janossy G, Papa S, Schmitz G, Lenkei R. Flow cytometric quantitation of immunofluorescence intensity: Problems and perspectives. Cytometry 1998;33:166-178.
5. Gritzapis AD, Baxevanis CN, Missitzis I, Katsanou ES, Alexis MN, Yotis J, Papamichail M. Quantitative fluorescence cytometric measurement of estrogen and progesterone receptors: Correlation with the hormone binding assay. Breast Cancer Res Treat 2003;80:1-13. (Pubitemid 36851280)
6. Schuerwegh AJ, Stevens WJ, Bridts CH, De Clerck LS. Evaluation of monensin and brefeldin A for flow cytometric determination of interleukin-1 beta, interleukin-6, and tumor necrosis factor-alpha in monocytes. Cytometry 2001;46:172-176.
7. Sklar LA, Finney DA. Analysis of ligand-receptor interactins with the fluorescence activated cell sorter. Cytometry 1982;3:161-165. (Pubitemid 13171822)
8. Lauer S, Goldstein B, Nolan RL, Nolan JP. Analysis of cholera toxin-ganglioside interactions by flow cytometry. Biochemistry 2002;41:1742-1751. (Pubitemid 34132248)
9. Chigaev A, Zwartz G, Graves SW, Dwyer DC, Tsuji H, Foutz TD, Edwards BS, Prossnitz ER, Larson RS, Sklar LA. a4b1 Integrin affinity changes govern cell adhesion. J Biol Chem 2003;278:38174-38182.
10. Brümmendorf TH, Maciejewski JP, Mak J, Young NS, Lansdorp PM. Telomere length in leukocyte subpopulations of patients with aplastic anemia. Blood 2001;97: 895-900. (Pubitemid 32154271)
11. Baerlocher GM, Lansdorp PM. Telomere length measurements in leukocyte subsets by automated multicolor flow-FISH. Cytometry Part A 2003;55A:1-6.
12. Marti GE, Gaigalas A, Voigt RE. Recent developments in quantitative fluorescence calibration for analyzing cells and microarrays. Cytometry 2000;42:263.
13. Resch-Genger U, Hoffmann K, Nietfeld W, Engel A, Neukammer J, Nitschke R, Ebert B, Macdonald R. How to improve quality assurance in fluorometry: Fluorescence-inherent sources of error and suited fluorescence standards. J Fluoresc 2005; 15:337-362. (Pubitemid 40963141)
14. Fricker M, Runions J, Moore I. Quantitative fluorescence microscopy: From art to science. Annu Rev Plant Biol 2006;57:79-107. (Pubitemid 44061019)
15. Hoffmann K, Resch-Genger U, Nitschke R. Comparability of fluorescence microscopy data and need for instrument characterization of spectral scanning microscopes. Springer Ser Fluoresc 2008;6:89-116.
16. Sisken JE. Fluorescent standards. Methods Cell Biol 1989;30:113-126.
17. Rost FWD. Quantitative Fluorescence Microscopy. Cambridge: Cambridge University Press; 1991. 236 p.
18. Tatarkova SA, Verma AK, Berk DA, Lloyd CJ. Quantitative fluorescence microscopy of macromolecules in gel and biological tissue. Phys Med Biol 2005;50: 5759-5768.
19. Biju V, Takeuchi M, Umemura K, Gad M, Ishikawa M. Fabrication of standard samples for single-molecule fluorescence imaging. Jpn J Appl Phys 2002;41:1579-1586. (Pubitemid 34750248)
20. Dustin ML, Golan DE, Zhu D-M, Miller JM, Meier W, Davies EA, Van Der Merwe PA. Low affinity interaction of human or rat t cell adhesion molecule cd2 with its ligand aligns adhering membranes to achieve high physiological affinity. J Biol Chem 1997;272:30889-30898. (Pubitemid 27527532)
21. Galush WJ, Nye JA, Groves JT. Quantitative fluorescence microscopy using supported lipid bilayer standards. Biophys J 2008;95:2512-2519.
22. Model MA, Healy KE. Quantification of the surface density of a fluorescent label with the optical microscope. J Biomed Mater Res 2000;50:90-96. (Pubitemid 30054142)
23. Poon SS, Wong JT, Saunders DN, Ma QC, Mckinney S, Fee J, Aparicio SA. Intensity calibration and automated cell cycle gating for high-throughput image-based siRNA screens of mammalian cells. Cytometry Part A 2008;73A:904-917.
24. Lockett SJ, Jacobson K, Herman B. Quantitative precision of an automated, fluorescence-based image cytometer. Anal Quant Cytol Histol 1992;14:187-202.
25. Sugiyama Y, Kawabata I, Sobue K, Okabe S. Determination of absolute protein numbers in single synapses by a GFP-based calibration technique. Nat Methods 2005;2:677-684. (Pubitemid 41223094)
26. Model MA, Khitrin AK, Blank JL. Measurement of the absorption of concentrated dyes and their use for quantitative imaging of surface topography. J Microsc 2008;231:156-167.
27. Mullins JM. Overview of fluorochromes. Methods Mol Biol 1999;115:97-105.
28. Lakowicz JR. Principles of fluorescence spectroscopy, 3rd ed. New York: Springer; 2006. 954 p.
29. Model MA, Burkhardt JK. A standard for calibration and shading correction of a fluorescence microscope. Cytometry 2001;44:309-316.
30. Model MA, Blank JL. Intensity calibration of a laser scanning confocal microscope based on concentrated dyes. Anal Quant Cytol Histol 2006;28:253-261. (Pubitemid 44622501)
31. Sharon N, Liz H. Lectins, 2nd ed. Dordrecht: Springer; 2007. 454 p.
32. Kline D. Quantitative microinjection of mouse oocytes and eggs. In: Carroll DJ, editor. Microinjection: Methods and Applications, Vol.518. New York: Humana Press; 2009. pp 135-156.
33. Bhavanandan VP, Katlic AW. The interaction of wheat germ agglutinin with sialoglycoproteins. J Biol Chem 1979;254:4000-4008.
34. Goldstein IJ, Hammarström S, Sundblad G. Precipitation and carbohydrate-binding specificity studies on wheat germ agglutinin. Biochim Biophys Acta 1975;405:53-61.
35. Ploem JC. Quantitative immunofluorescence. In: Holborow EJ, editor. Standardization in Immunofluorescence. Oxford: Blackwell Scientific Publications; 1970. pp 63-73.
36. Hiraoka Y, Sedat JW, Agard DA. Determination of the three-dimensional imaging properties of a light microscope system. Biophys J 1990;57:325-333.
37. Gordon A, Colman-Lerner A, Chin TE, Benjamin KR, Yu RC, Brent R. Single-cell quantification of molecules and rates using open-source microscope-based cytometry. Nat Methods 2007;4:175-181. (Pubitemid 46454687)
38. Diaspro A, Federici F, Robello M. Influence of refractive-index mismatch in high-resolution three-dimensional confocal microscopy. Appl Opt 2002;41: 685-690. (Pubitemid 34636194)
39. Masoumi Z, Yekta A, Masino J. Standards for Accurate Flat-Field Calibration in Wide-Field Fluorescence Microscopy. Geneva, Switzerland: 11th Annual Conference of the Society for Biomolecular Screening; September 11-15, 2005.
40. Ozinskas AJ. Principles of fluorescence immunoassay. In: Lakowicz JR, editor. Topics in Fluorescent Microscopy, Vol.4. New York: Plenum Press; 1994. pp 449-496.
41. Deka C, Lehnert BE, Lehnert NM, Jones GM, Sklar LA, Steinkamp JA. Analysis of fluorescence lifetime and quenching of FITC-conjugated antibodies on cells by phase-sensitive flow cytometry. Cytometry 1996;25:271-279. (Pubitemid 26386324)
42. Al-Hakiem MHH, Landon J, Smith DS, Nargessi RD. Fluorimetric assays for avidin and biotin based on biotin-induced fluorescence enhancement of fluorescein-labeled avidin. Anal Biochem 1981;116:264-267. (Pubitemid 12221193)
43. Emans N, Biwersi J, Verkman AS. Imaging of endosome fusion in BHK fibroblasts based on a novel fluorimetric avidin-biotin binding assay. Biophys J 1995;69: 716-728.
44. Panchuk-Voloshina N, Haugland RP, Bishop-Stewart J, Bhalgat MK, Millard PJ, Mao F, Leung W-Y, Haugland RP. Alexa dyes, a series of new fluorescent dyes that yield exceptionally bright, photostable conjugates. J Histochem Cytochem 1999;47: 1179-1188. (Pubitemid 29418323)
45. Marmé N, Knemeyer JP, Sauer M, Wolfrum J. Inter- and intramolecular fluorescence quenching of organic dyes by tryptophan. Bioconjug Chem 2003;14:1133-1139. (Pubitemid 37449040)