Intensity calibration and automated cell cycle gating for high-throughput image-based siRNA screens of mammalian cells

Cytometry Part A

Volumn 73, Issue 10, 2008, Pages 904-917

  Poon, Steven S S ^a   Wong, Jason T ^a   Saunders, Darren N ^a   Ma, Qianli C ^a   McKinney, Steven ^a   Fee, John ^a   Aparicio, Samuel A J R ^a,b  

^a BRITISH COLUMBIA CANCER RESEARCH CENTRE   (Canada)

^b UNIVERSITY OF BRITISH COLUMBIA   (Canada)

Author keywords

Automatic cell cycle gating; Background correction; Cell cycle gating; Fluorescence intensity calibration; High throughput cell cycle analysis; High throughput imaging; Image preprocessing

Indexed keywords

DNA; SMALL INTERFERING RNA;

ARTICLE; AUTOANALYSIS; CALIBRATION; CELL CYCLE REGULATION; CONTROLLED STUDY; FLUORESCENCE; HIGH THROUGHPUT SCREENING; HUMAN; HUMAN CELL; ILLUMINATION; IMAGE PROCESSING; IMAGING SYSTEM; PARAMETER; PRIORITY JOURNAL; SIGNAL NOISE RATIO;

ALGORITHMS; BROMODEOXYURIDINE; CALIBRATION; CELL CYCLE; CELL LINE, TUMOR; CELL SEPARATION; FLOW CYTOMETRY; GENE SILENCING; HUMANS; IMAGE PROCESSING, COMPUTER-ASSISTED; INDOLES; PROTEIN KINASES; RNA, SMALL INTERFERING; STAINING AND LABELING;

EID: 53049089846     PISSN: 15524922     EISSN: 15524930     Source Type: Journal    
DOI: 10.1002/cyto.a.20624     Document Type: Article

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