Determination of absolute protein numbers in single synapses by a GFP-based calibration technique

Nature Methods

Volumn 2, Issue 9, 2005, Pages 677-684

  Sugiyama, Yoshiko ^a,b   Kawabata, Izumi ^a   Sobue, Kenji ^c   Okabe, Shigeo ^a,b,d,e  

^a TOKYO MEDICAL AND DENTAL UNIVERSITY   (Japan)

^b TOKYO MEDICAL AND DENTAL UNIVERSITY   (Japan)

^c OSAKA UNIVERSITY GRADUATE SCHOOL OF MEDICINE   (Japan)

^d JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

^e NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY AIST   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; MICROSPHERE; POSTSYNAPTIC DENSITY PROTEIN 95; SCAFFOLD PROTEIN;

ANIMAL CELL; ARTICLE; CALIBRATION; CELL MATURATION; COMPLEX FORMATION; CONTROLLED STUDY; EMBRYO; EXCITATORY POSTSYNAPTIC POTENTIAL; FLUORESCENCE ANALYSIS; HIPPOCAMPUS; MACROMOLECULE; MATHEMATICAL COMPUTING; MOLECULAR MODEL; MOUSE; NERVE CELL CULTURE; NERVE CELL DIFFERENTIATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN CONTENT; PROTEIN STABILITY; QUANTITATIVE ANALYSIS; STOICHIOMETRY; SYNAPSE;

ANIMALS; CELLS, CULTURED; GENE EXPRESSION PROFILING; GREEN FLUORESCENT PROTEINS; HIPPOCAMPUS; MICE; MICROSCOPY, FLUORESCENCE; NERVE TISSUE PROTEINS; NEURONS; SYNAPSES; SYNAPTIC TRANSMISSION;

ANIMALIA;

EID: 24044487619     PISSN: 15487091     EISSN: None     Source Type: Journal    
DOI: 10.1038/nmeth783     Document Type: Article

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