Enhanced recombinant protein synthesis in batch and fed-batch Escherichia coli fermentation based on removal of inhibitory acetate by electrodialysis

Journal of Chemical Technology and Biotechnology

Volumn 84, Issue 9, 2009, Pages 1284-1291

  Wong, Michael ^a   Wright, Michael ^b   Woodley, John M ^c   Lye, Gary J ^a  

^a UNIVERSITY COLLEGE LONDON   (United Kingdom)

^b Isis Innovation   (United Kingdom)

^c TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

Author keywords

Electrodialysis; Escherichia coli; In situ acetate removal; Recombinant protein synthesis

Indexed keywords

ACETATE REMOVAL; BIOPROCESSES; DETRIMENTAL EFFECTS; FED BATCHES; FED-BATCH FERMENTATION; FERMENTATION BROTHS; GENERIC APPROACH; GENETIC APPROACH; GREEN FLUORESCENT PROTEIN; IN SITU ACETATE REMOVAL; IN-CELL; IN-SITU; MAXIMUM SPECIFIC GROWTH RATES; MODE OF OPERATIONS; PROCESS PRODUCTIVITY; PRODUCT YIELDS; RECOMBINANT ENZYMES; RECOMBINANT PROTEIN; RECOMBINANT PROTEIN PRODUCTIONS; RECOMBINANT PROTEIN SYNTHESIS; SINGLE CELLS; TANK BIOREACTORS;

BIOMASS; CELL GROWTH; CELL MEMBRANES; ELECTRODIALYSIS; ESCHERICHIA COLI; FERMENTATION; GROWTH KINETICS; ION EXCHANGE; ION EXCHANGE MEMBRANES; METAL RECOVERY; PRODUCTIVITY;

PROTEINS;

ACETIC ACID; GREEN FLUORESCENT PROTEIN; RECOMBINANT PROTEIN;

ARTICLE; BACTERIAL GROWTH; BATCH FERMENTATION; ELECTRODIALYSIS; ESCHERICHIA COLI; FED BATCH FERMENTATION; GROWTH RATE; MICROBIAL BIOMASS; NONHUMAN; PROTEIN SYNTHESIS; STIRRED REACTOR;

ESCHERICHIA COLI;

EID: 70349481485     PISSN: 02682575     EISSN: 10974660     Source Type: Journal    
DOI: 10.1002/jctb.2174     Document Type: Article

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