Sequential proteolysis and high-field FTICR MS to determine disulfide connectivity and 4-maleimide TEMPO spin-label location in L126C GM2 activator protein

Analytical Chemistry

Volumn 81, Issue 18, 2009, Pages 7611-7617

  Tipton, Jeremiah D ^a   Carter, Jeffrey D ^b   Mathias, Jordan D ^b   Emmett, Mark R ^a,c   Fanucci, Gail E ^b   Marshall, Alan G ^a,c  

^a FLORIDA STATE UNIVERSITY   (United States)

^b University of Florida ^*  (United States)

^c FLORIDA STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIVATOR PROTEINS; AUTOMATIC GAIN CONTROL; DISULFIDE BONDS; E. COLI; ELECTRON PARAMAGNETIC RESONANCE; FOURIER TRANSFORM ION CYCLOTRON RESONANCE MASS SPECTROMETRY; FT-ICR MS; GENETIC MUTATIONS; HIGH-FIELD; INCLUSION BODIES; LIPID BINDING; LOOP REGIONS; LYSOSOMAL STORAGE DISORDER; MALEIMIDES; MASS ACCURACY; MULTIPLE CONFORMATIONS; NON-ENZYMATIC; PROTEASE DIGESTION; SITE DIRECTED MUTAGENESIS; ULTRA-HIGH; WILD TYPES;

AMINES; CRYSTALLOGRAPHY; ELECTRON RESONANCE; ELECTROSPRAY IONIZATION; ESCHERICHIA COLI; GAIN CONTROL; MAGNETIC FIELD EFFECTS; MASS SPECTROMETRY; MINERALOGY; PARAMAGNETIC RESONANCE; PARAMAGNETISM; SPIN DYNAMICS;

PROTEIN FOLDING;

2,2,6,6 TETRAMETHYL 1 PIPERIDINYLOXY 4 MALEIMIDE; ASPARAGINE; ASPARTIC ACID; CYSTEINE; DISULFIDE; GANGLIOSIDE GM2 ACTIVATOR PROTEIN; GLUTAMIC ACID; MALEIMIDE DERIVATIVE; TRYPSIN; UNCLASSIFIED DRUG;

ARTICLE; CELL INCLUSION; CHEMICAL STRUCTURE; CONTROLLED STUDY; CRYSTALLOGRAPHY; DISULFIDE BOND; ELECTRON SPIN RESONANCE; ESCHERICHIA COLI; ION CYCLOTRON RESONANCE MASS SPECTROMETRY; LIQUID CHROMATOGRAPHY; MAGNETIC FIELD; NONHUMAN; PEPTIDE ANALYSIS; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN FOLDING; SITE DIRECTED MUTAGENESIS; SPIN LABELING;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; CRYSTALLOGRAPHY, X-RAY; CYCLIC N-OXIDES; DISULFIDES; ELECTRON SPIN RESONANCE SPECTROSCOPY; FOURIER ANALYSIS; G(M2) ACTIVATOR PROTEIN; MALEIMIDES; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PEPTIDES; PROTEIN FOLDING; RECOMBINANT PROTEINS; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; SPIN LABELS; TRYPSIN;

EID: 70349286679     PISSN: 00032700     EISSN: None     Source Type: Journal    
DOI: 10.1021/ac9009935     Document Type: Article

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