A continuous fluorigenic assay for the measurement of the activity of endoplasmic reticulum aminopeptidase 1: Competition kinetics as a tool for enzyme specificity investigation

Analytical Biochemistry

Volumn 395, Issue 1, 2009, Pages 33-40

  Evnouchidou, Irini ^a   Berardi, Marcelo J ^b   Stratikos, Efstratios ^a  

^a INSTITUTE OF NANOSCIENCE AND NANOTECHNOLOGY   (Greece)

^b HARVARD MEDICAL SCHOOL   (United States)

Author keywords

Aminopeptidase; Assay; Competition; Enzyme; Fluorescence; Immunity; Kinetics; Peptide; Presentation; Processing; Specificity

Indexed keywords

AMINO ACIDS; ASSAYS; COMPETITION; ENZYME KINETICS; ENZYMES; FLUORESCENCE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; KINETICS; PEPTIDES; PROCESSING;

AMINO PEPTIDASE; IMMUNITY; N-TERMINAL TRYPTOPHAN; PRESENTATION; SMALL MOLECULE INHIBITOR; SPECIFICITY; SUBSTRATE SPECIFICITY; TRYPTOPHAN FLUORESCENCE;

SUBSTRATES;

AMINOPEPTIDASE I; AMINOPEPTIDASE I INHIBITOR; ENZYME INHIBITOR; PEPTIDE; TRYPTOPHAN; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ARTICLE; CONTINUOUS FLUORIGENIC ASSAY; ENDOPLASMIC RETICULUM; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME ASSAY; ENZYME KINETICS; ENZYME SPECIFICITY; FLUORESCENCE ANALYSIS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; PRIORITY JOURNAL;

AMINOPEPTIDASES; BINDING, COMPETITIVE; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; DINITROPHENOLS; ENDOPLASMIC RETICULUM; FLUORESCENT DYES; HUMANS; KINETICS; LEAST-SQUARES ANALYSIS; METALLOPROTEASES; MODELS, BIOLOGICAL; OLIGOPEPTIDES; PROTEASE INHIBITORS; RECOMBINANT PROTEINS; SPECTROMETRY, FLUORESCENCE; SUBSTRATE SPECIFICITY; TRYPTOPHAN;

EID: 70349155824     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2009.07.032     Document Type: Article

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