Isolation of two cytochrome P450 forms, CYP2A19 and CYP1A, from pig liver microsomes

Journal of Veterinary Pharmacology and Therapeutics

Volumn 32, Issue 5, 2009, Pages 470-476

  Matal, J ^a   Tunkova A ^a   Siller M ^a   Anzenbacherova E ^a   Anzenbacher, P ^a  

^a PALACKÝ UNIVERSITY   (Czech Republic)

Author keywords

[No Author keywords available]

Indexed keywords

COUMARIN; CYTOCHROME P450; CYTOCHROME P450 1A; CYTOCHROME P450 1A2; CYTOCHROME P450 2A19; CYTOCHROME P450 2A6; DRUG RESIDUE; ENZYME; ETHOXYRESORUFIN; HYDROXYAPATITE; PROTEIN; SEPHAROSE; UNCLASSIFIED DRUG; VETERINARY DRUG; XENOBIOTIC AGENT;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BIOTRANSFORMATION; COLUMN CHROMATOGRAPHY; CONTROLLED STUDY; DRUG DETERMINATION; DRUG METABOLISM; ELECTROPHORESIS; ENZYME ACTIVITY; ENZYME ISOLATION; KINETICS; LIVER MICROSOME; MEAT; NONHUMAN; PRIORITY JOURNAL; PROTEIN PURIFICATION; PURIFICATION; SWINE; WESTERN BLOTTING;

ANIMALS; ARYL HYDROCARBON HYDROXYLASES; BLOTTING, WESTERN; CHROMATOGRAPHY, AGAROSE; CYTOCHROME P-450 CYP1A1; HUMANS; MICROSOMES, LIVER; SWINE;

RATTUS; SUIDAE; SUS;

EID: 70349154249     PISSN: 01407783     EISSN: 13652885     Source Type: Journal    
DOI: 10.1111/j.1365-2885.2009.01076.x     Document Type: Article

References (41)

1. Anzenbacher, P. Anzenbacherová, E. (2001) Cytochromes P450 and metabolism of xenobiotics. Cellular and Molecular Life Sciences, 58, 737 747.
2. Anzenbacher, P., Souček, P., Anzenbacherová, E., Gut, I., Hrubý, K., Svoboda, Z. Kvě tina, J. (1998) Presence and activity of cytochrome P450 isoforms in minipig liver microsomes. Comparison with human liver samples. Drug Metabolism and Disposition, 26, 56 59.
3. Anzenbacherová, E., Baranová, J., Zuber, R., Pechová, A., Anzenbacher, P., Souček, P. Martinková, J. (2005) Model systems based on experimental animals for studies on drug metabolism in man: (Mini) pig cytochromes P450 3A29 and 2E1. Basic & Clinical Pharmacology & Toxicology, 96, 244 245.
4. 3 25-hydroxylase from pig liver microsomes. The Biochemical Journal, 287, 725 731.
5. Baranová, J., Anzenbacherová, E., Anzenbacher, P. Souček, P. (2005) Minipig cytochrome P450 2E1: comparison with human enzyme. Drug Metabolism and Disposition, 33, 862 865.
6. Chamuleau, R.A., Poyck, P.P. van de Kerkhove, M.P. (2006) Bioartificial liver: its pros and cons. Therapeutic Apheresis and Dialysis, 10, 168 174.
7. Chang, T.K.H. Waxman, D.J. (1998) Enzymatic analysis of cDNA-expressed human CYP1A1, CYP1A2 and CYP1B1 with 7-ethoxyresorufin as a substrate. In Cytochrome P450 Protocols. Eds Phillips, I.R. Shephard, E.A., pp. 103 122. Humana Press, Totowa, New Jersey.
8. Chen, G., Cue, R.A., Lundstrom, K., Wood, J.D. Doran, O. (2008) Regulation of CYP2A6 protein expression by skatole, indole, and testicular steroids in primary cultured pig hepatocytes. Drug Metabolism and Disposition, 36, 56 60.
9. Chirulli, V., Marvasi, L., Zaghini, A., Fiorio, R., Longo, V. Gervasi, P.G. (2007) Inducibilityof AhR-regulated CYP genes by β-naphtoflavone in the liver, lung, kidney and heart of the pig. Toxicology, 240, 25 37.
10. Clement, B., Lomb, R. Möller, W. (1997) Isolation and characterization of the protein components of the liver microsomal O2-insensitive NADH-benzamidoxime reductase. The Journal of Biological Chemistry, 272, 19615 19620.
11. Diaz, G.J. Squires, E.J. (2000) Metabolism of 3-methylindole by porcine liver microsomes: responsible cytochrome P450 enzymes. Toxicological Sciences, 55, 284 292.
12. Distlerath, L.M., Reilly, P.E.B., Martin, M.V., Davis, G.G., Wilkinson, G.R. Guengerich, F.P. (1985) Purification and characterization of human liver cytochromes P-450 involved in debrisoquine 4-hydroxylation and phenacetin O-deethylation, two prototypes for genetic polymorphism in oxidative drug metabolism. Journal of Biological Chemistry, 260, 9057 9067.
13. Guengerich, F.P. (1997) Comparisons of catalytic selectivity of cytochrome P450 subfamily enzymes from different species. Chemico-Biological Interactions, 106, 161 182.
14. Guengerich, F.P. (2005) Human cytochrome P450 enzymes. In Cytochrome P450: Structure, Mechanism, and Biochemistry. Ed Ortiz de Montellano, P.R., pp. 377 530. Kluwer Academic/Plenum, New York.
15. Guengerich, F.P. Martin, M.V. (1998) Purification of cytochromes P450. In Cytochrome P450 Protocols. Eds Phillips, I.R. Shepard, E.A., pp. 35 53. Humana Press, Totowa, New Jersey.
16. Guengerich, F.P., Dannan, G.A., Wright, S.T., Martin, M.V. Kaminsky, L.S. (1982) Purification and characterization of liver microsomal cytochromes P-450: electrophoretic, spectral, catalytic and imunochemical properties and inducibility of eight isozymes isolated from rats treated with phenobarbital or β-naphtoflavone. Biochemistry, 21, 6019 6030.
17. Hansen, L.L., Larsen, A.E. Hansen-Møller, J. (1995) Influence of keeping pigs heavily fouled with faeces plus urine on skatole and indole concentration (boar taint) in subcutaneous fat. Acta agriculturæ Scandinavica, 45, 178 185.
18. Hara, H., Gridelli, B., Lin, Y.J., Marcos, A. Cooper, D.K. (2008) Liver xenografts for the treatment of acute liver failure: clinical and experimental experience and remaining immunologic barriers. Liver Transplantation, 14, 425 434.
19. Higashi, E., Nakajima, M., Katoh, M., Tokudome, S. Yokoi, T. (2007) Inhibitory effects of neurotransmitters and steroids on human CYP2A6. Drug Metabolism and Disposition, 35, 508 514.
20. Koenigs, L.L., Peter, R.M., Thompson, S.J., Rettie, A.E. Trager, W.F. (1997) Mechanism-based inactivation of human liver cytochrome P450 2A6 by 8-methoxypsoralen. Drug Metabolism and Disposition, 12, 1407 1415.
21. Kojima, M. Morozumi, T. (2004) Cloning of six full-lenght cDNAs encoding pig cytochrome P450 enzymes and gene expression of these enzymes in the liver and kidney. Journal of Health Science, 50, 518 529.
22. Kunze, K.L. Trager, W.F. (1993) Isoform-selective mechanism-based inhibition of human cytochrome P450 1A2 by furafylline. Chemical research in toxicology, 6, 649 656.
23. Levin, O. (1962) Column chromatography of proteins: calcium phosphate. Methods in Enzymology, 5, 27 32.
24. Ma, Q. Lu, A.Y.H. (2007) CYP1A induction and human risk assessment: an evolving tale of in vitro and in vivo studies. Drug Metabolism and Disposition, 35, 1009 1016.
25. Monshouwer, M., van't Klooster, G.A.E., Nijmeijer, S.M., Witkamp, R.F. van Miert, A.S. (1998) Characterization of cytochrome P450 isoenzymes in primary cultures of pig hepatocytes. Toxicology In Vitro, 12, 715 723.
26. Myers, M.J., Farrell, D.E., Howard, K.D. Kawalek, J.C. (2001) Identification of multiple constitutive and inducible hepatic cytochrome P450 enzymes in market weight swine. Drug Metabolism and Disposition, 29, 908 915.
27. Omura, T. Sato, R. (1964) The carbon-monoxide binding protein of liver microsomes. I. Evidences for its hemoprotein nature. The Journal of Biological Chemistry, 293, 2370 2378.
28. Raunio, H., Rautio, A., Gullstén, H. Pelkonen, O. (2001) Polymorphisms of CYP2A6 and its practical consequences. British Journal of Clinical Pharmacology, 52, 357 363.
29. Reeves, P.T. (2007) Residues of veterinary drugs at injection sites. Journal of Veterinary Pharmacology and Therapeutics, 30, 1 17.
30. Sakuma, T., Igarashi, T., Hieda, M., Ohgiya, S., Isogai, M., Ninomya, S., Nagata, R., Nemoto, N. Kamataki, M. (1997) Marmoset CYP1A2: primary structure and constitutive expression in livers. Carcinogenesis, 18, 1985 1991.
31. Sakuma, T., Hieda, M., Igarashi, T., Ohgiya, S., Nagata, R., Nemoto, N. Kamataki, T. (1998) Molecular cloning and functional analysis of cynomolgus monkey CYP1A2. Biochemical Pharmacology, 56, 131 139.
32. Savlik, M., Fimanova, K., Szotakova, B., Lamka, J. Skalova, L. (2006) Modulation of porcine biotransformation enzymes by anthelmintic therapy with fenbendazole and flubendazole. Research in Veterinary Science, 80, 267 274.
33. Skaanild, M.T. (2006) Porcine cytochrome P450 and metabolism. Current Pharmaceutical Design, 12, 1421 1427.
34. Skaanild, M.T. Friis, C. (1999) Cytochrome P450 sex differences in minipigs and conventional pigs. Pharmacology & Toxicology, 85, 174 180.
35. Skaanild, M.T. Friis, C. (2005) Porcine CYP2A Polymorphism and activity. Basic & Clinical Pharmacology & Toxicology, 97, 115 121.
36. Souček, P., Zuber, R., Anzenbacherová, E., Anzenbacher, P. Guengerich, F.P. (2001) Minipig cytochrome P450 3A, 2A and 2C enzymes have similar properties to human analogs. BMC Pharmacology, 1, 1 11.
37. Terner, M.A., Gilmore, W.J., Lou, Y. Squires, E.J. (2006) The role of CYP2A and CYP2E1 in metabolism of 3-methylindole in primary cultured porcine hepatocytes. Drug Metabolism and Disposition, 34, 848 854.
38. Towbin, H., Staehelin, T. Gordon, J. (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences of the United States of America, 76, 4350 4353.
39. Vaclaviková, R., Souček, P., Svobodová, L., Anzenbacher, P., Šimek, P., Guengerich, F.P. Gut, I. (2004) Different in vitro metabolism of paclitaxel and docetaxel in humans, rats, pigs, and minipigs. Drug Metabolism and Disposition, 32, 666 674.
40. Waxman, D.J. Chang, T.K. (1998) Spectrofluorimetric analysis of CYP2A6-catalyzed coumarin 7-hydroxylation. In Cytochrome P450 Protocols. Eds Phillips, I.R. Shephard, E.A., pp. 111 116. Humana Press, Totowa, New Jersey.
41. Yasukochi, Y. Masters, B.S. (1976) Some properties of a detergent-solubilized NADPH-cytochrome c(cytochrome P-450) reductase purified by biospecific affinity chromatography. The Journal of Biological Chemistry, 251, 5337 5344.