Minipig cytochrome P450 3A, 2A and 2C enzymes have similar properties to human analogs

BMC Pharmacology

Volumn 1, Issue , 2001, Pages

  Soucek, Pavel ^a   Zuber, Roman ^b   Anzenbacherová, Eva ^b   Anzenbacher, Pavel ^b   Guengerich, F Peter ^c  

^a NATIONAL INSTITUTE OF PUBLIC HEALTH   (Czech Republic)

^b PALACKÝ UNIVERSITY   (Czech Republic)

^c VANDERBILT UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

4 NITROPHENOL; BUFURALOL; COUMARIN; CYTOCHROME P450 2A; CYTOCHROME P450 2A6; CYTOCHROME P450 2C; CYTOCHROME P450 2C9; CYTOCHROME P450 2D6; CYTOCHROME P450 2E1; CYTOCHROME P450 3A; CYTOCHROME P450 3A4; DRUG METABOLIZING ENZYME; ISOENZYME; NIFEDIPINE; POLYCLONAL ANTIBODY; TOLBUTAMIDE;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ANIMAL CELL; ARTICLE; CONTROLLED STUDY; DRUG HYDROXYLATION; DRUG METABOLISM; DRUG OXIDATION; ENZYME ACTIVITY; ENZYME PURIFICATION; ENZYME SUBSTRATE; EXPERIMENTAL MODEL; HUMAN; HUMAN CELL; IMMUNOBLOTTING; IMMUNOHISTOCHEMISTRY; LIVER MICROSOME; NONHUMAN; ORTHOLOGY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN ANALYSIS; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY; SWINE; TOXICOKINETICS; XENOGRAFT;

EID: 2442708468     PISSN: 14712210     EISSN: None     Source Type: Journal    
DOI: 10.1186/1471-2210-1-11     Document Type: Article

References (32)

1. Ortiz de Montellano PR, (editor): Cytochrome P450 (2nd Ed.). New York, Plenum Press 1995
2. Guengerich FP: Metabolism of chemical carcinogens. Carcinogenesis 2000, 21:345-351
3. Anzenbacher P, Anzenbacherová E: Cytochromes P450 and metabolism of xenobiotics. CMLS, Cell. Mol. Life Sci 2001, 58:737-747
4. Anzenbacher P, Soucek P, Anzenbacherová E, Gut I, Hrubý K, Svoboda Z, Kvetina J: Presence and activity of cytochrome P450 isoforms in minipig liver microsomes. Comparison with human liver samples. Drug Metab. Dispos 1998, 26:90-93
5. Marini S, Longo V, Mazzaccaro A, Gervasi PG: Xenobiotic-metabolizing enzymes in pig nasal and hepatic tissues. Xenobiotica 1998, 28:923-935
6. Monshouwer M, van't Klooster GAE, Nijmeijer SM, Witkamp RF, van Miert ASJPAM: Characterization of cytochrome P450 isoenzymes in primary cultures of pig hepatocytes. Toxicol. in Vitro 1998, 12:715-723
7. Desille M, Corcos L, L'Helgoualc'h A, Frémond B, Campion J-P, Guillouzo A, Clément B: Detoxifying activity in pig livers and hepatocytes intended for xenotherapy. Transplantation 1999, 10:1437-1443
8. Skaanild M, Friis C: Cytochrome P450 sex differences in minipigs and conventional pigs. Pharm. Toxicol 1999, 85:174-180
9. Horslen SP, Hammel JM, Fristoe LW, Kangas JA, Collier DS, Sudan DL, Langnas AN, Dixon RS, Prentice ED, Shaw BW Jr, Fox IJ: Extracorporeal liver perfusion using human and pig livers for acute liver failure. Transplantation 2000, 70:1472-1478
10. Kvetina J, Svoboda Z, Nobilis M, Pastera J, Anzenbacher P: Experimental Goettingen minipig and Beagle dog as two species used in bioequivalence studies for clinical pharmacology. Gen. Physiol. Biophys 1999, 18:80-85
11. Donato MT, Castell JV, Gomez-Lechon MJ: Characterization of drug metabolizing activities in pig hepatocytes for use in bioartificial liver devices. J. Hepatol 1999, 31:542-549
12. Postlind H, Axén E, Bergman T, Wikvall K: Cloning, structure and expression of cDNA encoding vitamin D3-25 hydroxylase. Biochem. Biophys. Res. Commun 1997, 241:491-497
13. Nissen PH, Wintero AK, Fredholm M: Mapping of porcine genes belonging to two different cytochrome P450 subfamilies. Anim. Genet 1998, 29:7-11
14. Jurima-Romet M, Calsley WL, Leblanc CA, Nowakowska M: Evidence for the catalysis of dextromethorphan O-demethylation by a CYP2D6-like enzyme in pig liver. Toxicol. in Vitro 2000, 14:253-263
15. Skaanild MT, Friis C: Expression changes of CYP2A and CYP3A in microsomes from pig liver and cultured hepatocytes. Pharm. Toxicol 2000, 87:174-178
16. Lampen A, Christians U, Guengerich FP, Watkins PB, Kolars JC, Bader A, Gonschior AK, Dralle H, Hackbarth I, Sewing KF: Metabolism of the immunosuppressant tacrolimus in the small intestine: Cytochrome P450, drug interactions, and interindividual variability. Drug. Metab. Dispos 1995, 12:1315-1324
17. Olsen A, Hansen KT, Friis C: Pig hepatocytes as an in vitro model to study the regulation of human CYP3A4: prediction of drug-drug interactions with 17β-ethynylestradiol. Chem.-Biol. Interact 1997, 107:93-108
18. Bader A, Hansen T, Kirchner G, Allmeling C, Haverich A, Borlak JT: Primary porcine enterocyte and hepatocyte cultures to study drug oxidation reactions. Br. J. Pharmacol 2000, 129:331-342
19. Soucek P, Martin MV, Ueng YF, Guengerich FP: Identification of common cytochrome P450 epitope near the conserved heme-binding peptide with antibodies raised against recombinant cytochrome P450 family 2 proteins. Biochemistry 1995, 34:16013-16021
20. Sandhu P, Baba T, Guengerich FP: Expression of modified cytochrome 450 2C10(2C9) in Escherichia coli, purification, and reconstitution of catalytic activity. Arch. Biochem. Biophys 1993, 306:443-450
21. Gillam EJ, Baba T, Kim BR, Ohmori S, Guengerich FP: Expression of modified human cytochrome P450 3A4 in Escherichia coli and purification and reconstitution of the enzyme. Arch. Biochem. Biophys 1993, 305:123-131
22. Soucek P: Expression of cytochrome P4502A6 in Escherichia coli, purification, spectral, and catalytic characterization and preparation of polyclonal antibodies. Arch. Biochem. Biophys 1999, 370:190-200
23. Lake BG: Preparation and characterisation of microsomal fractions for studies on xenobiotic metabolism. In: Biochemical Toxicology. A practical approach (Edited by Snell K, Mullock B) Oxford, IRL Press 1990183-215
24. Guengerich FP, Dannan GA, Wright ST, Martin MV, Kaminsky LS: Purification and characterization of liver microsomal cytochromes P-450: electrophoretic, spectral, catalytic, and immunochemical properties and inducibility of eight isozymes isolated from rats treated with phenobarbital or beta naphthoflavone. Biochemistry 1982, 21:6019-6030
25. Guengerich FP: Analysis and characterization of enzymes. In: Principles and Methods in Toxicology (edited by Hayes A W, 3rd Ed.) New York, Raven Press 19941259-1313
26. Guengerich FP, Martin MV, Beaune PR, Kremers P, Wolff T, Waxman DJ: Characterization of rat and human liver microsomal cytochrome P-450 forms involved in nifedipine oxidation, prototype for genetic polymorphism in oxidative drug metabolism. J. Biol. Chem 1986, 261:5051-5060
27. Soucek P: Novel sensitive high performance liquid chromatographic method for assay of coumarin 7-hydroxylation. J. Chromatogr. B 1999, 734:23-29
28. Knodell RG, Hall SD, Wilkinson GR, Guengerich FP: Hepatic metabolism of tolbutamide: Characterization of the form of the cytochrome P450 involved in methyl-hydroxylation and relationship to in vivo disposition. J. Pharm. Exp. Ther 1987, 241:1112-1119
29. Shimada T, Yamazaki H: Cytochrome P450 reconstitution systems. In: Cytochrome P450 Protocols (edited by IR Phillips and EA Shephard) Totowa, NJ, Humana Press 199885-93
30. Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970, 227:680-685
31. Fairbanks G, Steck TL, Wallach D: Electrophoretic analysis of the major polypeptides of human erythrocyte membrane. Biochemistry 1971, 10:2606-2617
32. Towbin H, Staehelin T, Gordon J J: Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA 1979, 76:4350-4356