The chlorocatechol degradative genes, tfdT-CDEF, of Burkholderia sp. strain NK8 are involved in chlorobenzoate degradation and induced by chlorobenzoates and chlorocatechols

Gene

Volumn 268, Issue 1-2, 2001, Pages 207-214

  Liu, Shenghao ^a   Ogawa, Naoto ^a   Miyashita, Kiyotaka ^b  

^a NATIONAL INSTITUTE FOR AGRO ENVIRONMENTAL SCIENCES   (Japan)

^b Forestry Agency   (Japan)

Author keywords

Chlorocatechol 1,2 dioxygenase; Inducer of gene expression; lacZ transcriptional fusion; LysR type transcriptional regulator; Modified ortho pathway

Indexed keywords

2 CHLOROBENZOIC ACID; 2,4 DICHLOROPHENOXYACETIC ACID; 3 CHLOROBENZOIC ACID; 3 CHLOROCATECHOL; 3,4 DICHLOROCATECHOL; 4 CHLOROBENZOIC ACID; 4 CHLOROCATECHOL; BENZOIC ACID DERIVATIVE; CARBOXYMETHYLENEBUTENOLIDASE; CATECHOL DERIVATIVE; CHLOROCATECHOL 1,2 DIOXYGENASE; CHLOROMUCONATE CYCLOISOMERASE; KETONE DERIVATIVE; MALEYLACETATE REDUCTASE; UNCLASSIFIED DRUG;

ARTICLE; BURKHOLDERIA; ENZYME DEGRADATION; GENE CLUSTER; GENETIC ANALYSIS; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID; PRIORITY JOURNAL; REPORTER GENE; TRANSCRIPTION REGULATION; WAUTERSIA EUTROPHA;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; BURKHOLDERIA; CARBOXYLIC ESTER HYDROLASES; CATECHOLS; CHLOROBENZOATES; CLONING, MOLECULAR; DIOXYGENASES; GENE EXPRESSION REGULATION, BACTERIAL; INTRAMOLECULAR LYASES; MOLECULAR SEQUENCE DATA; OXIDOREDUCTASES; OXIDOREDUCTASES ACTING ON CH-CH GROUP DONORS; OXYGENASES; PROMOTER REGIONS (GENETICS); SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY, AMINO ACID; SUBSTRATE SPECIFICITY; TRANS-ACTIVATORS;

EID: 0035795590     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1119(01)00435-8     Document Type: Article

References (29)

1. Bagdasarian M., Lurz R., Rückert B., Franklin F.C.H., Bagdasarian M.M., Frey J., Timmis K.N. Specific-purpose plasmid cloning vectors. II. Broad host range, high copy number, RSF1010-derived vectors, and a host-vector system for gene cloning in Pseudomonas. Gene. 16:1981;237-247.
2. Bhat M.A., Ishida T., Horiike K., Vaidyanathan C.S., Nozaki M. Purification of 3,5-dichlorocatechol 1,2-dioxygenase, a nonheme iron dioxygenase and a key enzyme in the biodegradation of a herbicide, 2,4-dichlorophenoxyacetic acid (2,4-D), from Pseudomonas cepacia CSV90. Arch. Biochem. Biophy. 300:1993;738-746.
3. Bullock W.O., Fernandez J.M., Short J.M. XL1-Blue: a high efficiency plasmid transforming recA Escherichia coli strain with beta-galactosidase selection. BioTechniques. 5:1987;376-379.
4. Coco W.M., Rothmel R.K., Henikoff S., Chakrabarty A.M. Nucleotide sequence and initial functional characterization of the clcR gene encoding a LysR family activator of the clcABD chlorocatechol operon in Pseudomonas putida. J. Bacteriol. 175:1993;417-427.
5. Dorn E., Knackmuss H.-J. Chemical structure and biodegradability of halogenated aromatic compounds. Substitutent effects on 1,2-dioxygenation of catechol. Biochem. J. 174:1978;85-94.
6. Farinha M.A., Kropinski A.M. Construction of broad-host-range plasmid vectors for easy visible selection and analysis of promoters. J. Bacteriol. 172:1990;3496-3499.
7. Filer K., Harker A.R. Identification of the inducing agent of the 2,4-dichlorophenoxyacetic acid pathway encoded by plasmid pJP4. Appl. Environ. Microbiol. 63:1997;317-320.
8. Francisco P.B. Jr, Ogawa N., Suzuki K., Miyashita K. The chlorobenzoate dioxygenase genes of Burkholderia sp. strain NK8 involved in the catabolism of chlorobenzoates. Microbiology. 147:2001;121-133.
9. Frantz B., Chakrabarty A.M. Organization and nucleotide sequence determination of a gene cluster involved in 3-chlorocatechol degradation. Proc. Natl. Acad. Sci. USA. 84:1987;4460-4464.
10. Hanahan D. Techniques for transformation of E. coli. Glover D.M. DNA Cloning. 1985;109-135 IRL Press, Oxford.
11. Leander, M, Fulthorpe, R. 1997. Diversity of chlorocatechol dioxygenases isolated from pristine soil chlorobenzoate degraders. In: Proceedings of the 47th Annual Meeting of the Canadian Society of Microbiology, Univertité Laval, Que. 15-19 June. Abstract Emp10.
12. Leander M., Vallaeys T., Fulthorpe R. Amplification of putative chlorocatechol dioxygenase gene fragments from α- and β-proteobacteria. Can. J. Microbiol. 44:1998;482-486.
13. Leveau J.H.J., van der Meer J.R. The tfdR gene product can successfully take over the role of the insertion element-inactivated TfdT protein as a transcriptional activator of the tfdCDEF gene cluster, which encodes chlorocatechol degradation in Ralstonia eutropha JMP134(pJP4). J. Bacteriol. 178:1996;6824-6832.
14. Matrubutham U., Harker A.R. Analysis of duplicated gene sequences associated with tfdR and tfdS in Alcaligenes eutrophus JMP134. J. Bacteriol. 176:1994;2348-2353.
15. McFall S.M., Parsek M.R., Chakrabarty A.M. 2-Chloromuconate and ClcR-mediated activation of the clcABD operon: in vitro transcriptional and DNase I footprint analyses. J. Bacteriol. 179:1997;3655-3663.
16. McFall S.M., Chugani S.A., Chakrabarty A.M. Transcriptional activation of the catechol and chlorocatechol operons: variations on a theme. Gene. 223:1998;257-267.
17. Ogawa N., Miyashita K. Recombination of a 3-chlorobenzoate catabolic plasmid from Alcaligenes eutrophus NH9 mediated by direct repeat elements. Appl. Environ. Microbiol. 61:1995;3788-3795.
18. Ogawa N., Miyashita K. The chlorocatechol-catabolic transposon Tn5707 of Alcaligenes eutrophus NH9, carrying a gene cluster highly homologous to that in the 1,2,4-trichlorobenzene-degrading bacterium Pseudomonas sp. strain P51, confers the ability to grow on 3-chlorobenzoate. Appl. Environ. Microbiol. 65:1999;724-731.
19. Ogawa N., McFall S.M., Klem T.J., Miyashita K., Chakrabarty A.M. Transcriptional activation of the chlorocatechol degradative genes of Ralstonia eutropha NH9. J. Bacteriol. 181:1999;6697-6705.
20. - bacteria. Gene. 133:1993;23-30.
21. Perkins E.J., Gordon M.P., Caceres O., Lurquin P.F. Organization and sequence analysis of the 2,4-dichlorophenol hydroxylase and dichlorocatechol oxidative operons of plasmid pJP4. J. Bacteriol. 172:1990;2351-2359.
22. Ravatn R., Studer S., Zehnder A.J.B., van der Meer J.R. Int-B13, an unusual site-specific recombinase of the bacteriophage P4 integrase family, is responsible for chromosomal insertion of the 105- kilobase clc element of Pseudomonas sp. strain B13. J. Bacteriol. 180:1998;5505-5514.
23. Sambrook J., Fritsch E.F., Maniatis T. Molecular Cloning, A Laboratory Manual. 2nd Edition :1989;Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
24. Schell M.A. Molecular biology of the LysR family of transcriptional regulators. Annu. Rev. Microbiol. 47:1993;597-626.
25. Simon R., Priefer U., Pühler A. A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in gram-negative bacteria. BioTechnology. 1:1983;784-791.
26. Vallaeys T., Courde L., Mc Gowan C., Wright A.D., Fulthorpe R.R. Phylogenetic analyses indicate independent recruitment of diverse gene cassettes during assemblage of the 2,4-D catabolic pathway. FEMS Microbial. Ecol. 28:1999;373-382.
27. van der Meer J.R., Eggen R.I., Zehnder A.J.B., de Vos W.M. Sequence analysis of the Pseudomonas sp. strain P51 tcb gene cluster, which encodes metabolism of chlorinated catechols: evidence for specialization of catechol 1,2-dioxygenases for chlorinated substrates. J. Bacteriol. 173:1991;2425-2434.
28. van der Meer J.R., Frijters A.C., Leveau J.H.J., Eggen R.I., Zehnder A.J.B., de Vos W.M. Characterization of the Pseudomonas sp. strain P51 gene tcbR, a LysR- type transcriptional activator of the tcbCDEF chlorocatechol oxidative operon, and analysis of the regulatory region. J. Bacteriol. 173:1991;3700-3708.
29. Yanisch-Perron C., Vieira J., Messing J. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene. 33:1985;103-119.