Increased expression of the nitric oxide synthase gene and protein in corpus cavernosum by repeated dosing of udenafil in a rat model of chemical diabetogenesis

Asian Journal of Andrology

Volumn 11, Issue 4, 2009, Pages 435-442

  Ahn, G J ^a   Chung, H K ^a   Lee, C H ^a   Kang, K K ^a   Ahn, B O ^a  

^a Dong A Pharmaceutical Company   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

CYCLIC GMP; ENDOTHELIAL NITRIC OXIDE SYNTHASE; GLUCOSE; NEURONAL NITRIC OXIDE SYNTHASE; NITRIC OXIDE SYNTHASE; UDENAFIL;

ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; BODY WEIGHT; CONTROLLED STUDY; CORPUS CAVERNOSUM; DIABETES CONTROL; DIABETES MELLITUS; DIABETOGENESIS; DISEASE ASSOCIATION; DOWN REGULATION; DRUG DOSE COMPARISON; DRUG MEGADOSE; ENZYME DEGRADATION; ERECTILE DYSFUNCTION; GLUCOSE BLOOD LEVEL; IMMUNOBLOTTING; LONG TERM CARE; LOW DRUG DOSE; MALE; NONHUMAN; PENIS ERECTION; PROTEIN EXPRESSION; PROTEIN FUNCTION; RAT; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

ANIMALS; BASE SEQUENCE; BLOOD GLUCOSE; BODY WEIGHT; DIABETES MELLITUS, EXPERIMENTAL; DISEASE MODELS, ANIMAL; DNA PRIMERS; ELECTRIC STIMULATION; ERECTILE DYSFUNCTION; GENE EXPRESSION REGULATION, ENZYMOLOGIC; MALE; NITRIC OXIDE SYNTHASE TYPE I; PENIS; PHOSPHODIESTERASE INHIBITORS; POLYMERASE CHAIN REACTION; PYRIMIDINES; RATS; RATS, SPRAGUE-DAWLEY; RNA, MESSENGER; SULFONAMIDES;

EID: 69749116587     PISSN: 1008682X     EISSN: 17457262     Source Type: Journal    
DOI: 10.1038/aja.2009.27     Document Type: Article

References (36)

1. Dunsmuir WD, Holmes SAV. The aetiology and management of erectile, ejaculatory and fertility problems in men with diabetes mellitus. Diabet Med 1996; 13: 700-8.
2. Burnett AL, Lowenstein CJ, Bredt DS, Chang TS, Snyper SH. Nitric oxide: a physiologic mediator of penile erection. Science 1992; 257: 401-3.
3. Jung HC, Mun KH, Park TC, Lee YC, Park JM, et al. Role of nitric oxide in penile erection. Yonsei Med J 1997; 38: 261-9.
4. Andersson KE. Pharmacology of penile erection. Pharmacol Rev 2001; 53: 417-50.
5. Hedlund P, Ny L, Alm P, Andersson KE. Cholinergic nerves in human corpus cavernosum and spongiosum contain nitric oxide synthase and heme oxygenase. J Urol 2000; 164: 868-75.
6. Gonzalez CM, Brannigan RE, Bervig T, Zelner D, Podlasek CA, et al. Protein and gene expression of nitric oxide synthase isoforms I and III in the rat penile shaft. J Androl 2001; 22: 54-61.
7. Vernet D, Cai L, Garban H, Babbitt ML, Murray FT, et al. Reduction of penile nitric oxide synthase in diabetic BB/WORdp (type I) and BBZ/ WORdp (type II) rats with erectile dysfunction. Endocrinology 1995; 136: 5709-17.
8. Cellek S, Rodrigo J, Lobos E, Fernandez P, Serrano J, et al. Selective nitrergic neurodegeneration in diabetes mellitus - a nitric oxide-dependent phenomenon. Br J Pharmacol 1999; 128: 1804-12.
9. El-Sakka AI, Lin CS, Chui RM, Dahiya R, Lue TF. Effects of diabetes on nitric oxide synthase and growth factor genes and protein expression in an animal model. Int J Impot Res 1999; 11: 123-32.
10. Escrig A, Marin R, Abreu P, Gonzalez-Mora JL, Mas M. Changes in mating behavior, erectile function, and nitric oxide levels in penile corpora cavernosa in streptozotocin-diabetic rats. Biol Reprod 2002; 66: 185-9.
11. Akingba AG, Burnett AL. Endothelial nitric oxide synthase protein expression, localization, and activity in the penis of the alloxan-induced diabetic rat. Mol Urol 2001; 5: 189-97.
12. Christian B. Nitric oxide and the immune response. Nat Immunol 2001; 2: 907-16.
13. Kawano K, Hirashima T, Mori S, Saitoh Y, Kurosumi M, et al. Spontaneous long-term hyperglycemic rat with diabetic complications. Otsuka Long-Evans Tokushima Fatty (OLETF) strain. Diabetes 1992; 41: 1422-8.
14. Jesmin S, Sakuma I, Salah-Eldin A, Nonomura K, Hattori Y, et al. Diminished penile expression of vascular endothelial growth factor and its receptors at the insulin-resistant stage of a type II diabetic rat model: A possible cause for erectile dysfunction in diabetes. J Mol Endocrinol 2003; 31: 401-18.
15. Zheng H, Bidasee KR, Mayhan WG, Patel KP. Lack of central nitric oxide triggers erectile dysfunction in diabetes. Am J Physiol Regul Integr Comp Physiol 2007; 292: R1158-64.
16. Ahn GJ, Sohn YS, Kang KK, Ahn BO, Kwon JW, et al. The effect of PDE5 inhibition on the erectile function in streptozotocin-induced diabetic rats. Int J Impot Res 2005; 17: 134-41.
17. Yu JY, Kang KK, Yoo M. Erectile potentials of a new phosphodiesterase type 5 inhibitor, DA-8159, in diet-induced obese rats. Asian J Androl 2006; 8: 325-9.
18. Bivalacqua TJ, Hellstrom WJ, Kadowitz PJ, Champion HC. Increased expression of arginase II in human diabetic corpus cavernosum: In diabetic-associated erectile dysfunction. Biochem Biophys Res Commun 2001; 283: 923-7.
19. Mori M, Gotoh T. Regulation of nitric oxide production by arginine metabolic enzymes. Biochem Biophys Res Commun 2000; 275: 715-9.
20. Cox MB, Kim NN, Traish AM, Christianson DW. Arginaseboronic acid complex highlights a physiological role in erectile function. Nat Struct Biol 1999; 6: 1043-7.
21. Ganz MB, Seftel A. Glucose-induced changes in protein kinase C and nitric oxide are prevented by vitamin E. Am J Physiol Endocrinol Metab 2000; 278: 146-52.
22. Ming XF, Viswambharan H, Barandier C, Ruffieux J, Kaibuchi K, et al. Rho GTPase/Rho kinase negatively regulates endothelial nitric oxide synthase phosphorylation through the inhibition of protein kinase B/Akt in human endothelial cells. Mol Cell Biol 2002; 24: 8467-77.
23. Bivalacqua TJ, Champion HC, Usta MF, Cellek S, Chitaley K, et al. RhoA/Rho-kinase suppresses endothelial nitric oxide synthase in the penis: A mechanism for diabetes-associated erectile dysfunction. Proc Natl Acad Sci USA 2004; 101: 9121-6.
24. Yamanaka M, Shirai M, Shiina H, Tanaka Y, Tsujimura A, et al. Diabetes induced erectile dysfunction and apoptosis in penile crura are recovered by insulin treatment in rats. J Urol 2003; 170: 291-7.
25. Cartiledge JJ, Eardley I, Morrison JFB. Impairment of corpus cavernosal smooth muscle relaxation by glycosylated human hemoglobin. BJU Int 2000; 85: 735-41.
26. Usta MF, Bivalacqua TJ, Yang DY, Ramamitharan A, Sell DR, et al. The protective effect of aminoguanidine on erectile function in streptozotocin diabetic rats, J Urol 2003; 170: 1437-42.
27. Khan MA, Thompson CS, Jeremy JY, Mumtaz FH, Mikhailidis P, et al. The effect of superoxide dismutase on nitric oxide mediated and electrical field stimulated diabetic rabbit cavernosal smooth muscle relaxation. BJU Int 2001; 87: 98-103.
28. Dimitriadis F, Giannakis D, Pardalidis N, Zikopoulos K, Paraskevaidis E, et al. Effects of phosphodiesterase-5 inhibitors on sperm parameters and fertilizing capacity. Asian J Androl 2008; 10: 115-33.
29. Milani E, Nikfar S, Khorasani R, Zamani MJ, Abdollahi M. Reduction of diabetes-induced oxidative stress by phosphodiesterase inhibitors in rats. Comp Biochem Physiol C Toxicol Pharmacol 2005; 140: 251-5.
30. Preston IR, Klinger JR, Houtches J, Nelson D, Farber HW, et al. Acute and chronic effects of sildenafil in patients with pulmonary arterial hypertension. Respir Med 2005; 99: 1501-10.
31. Takimoto E, Champion HC, Li M, Belardi D, Ren S, et al. Chronic inhibition of cyclic GMP phosphodiesterase 5A prevents and reverses cardiac hypertrophy. Nat Med 2005; 2: 214-22.
32. Musicki B, Champion HC, Becker RE, Liu T, Kramer MF, et al. Erection capability is potentiated by long-term sildenafil treatment: role of blood flow-induced endothelial nitric- oxide synthase phosphorylation. Mol Pharmacol 2005; 68: 226-32.
33. Behr-Roussel D, Gorny D, Mevel K, Caisey S, Bernabe J, et al. Chronic sildenafil improves erectile function and endothelium-dependent cavernosal relaxations in rats: Lack of tachyphylaxis. Eur Urol 2005; 47: 87-91.
34. Aziz MT, Al-Asmar MF, Mostafa T, Atta H, Rashed L, et al. Assessment of heme oxygenase-1 (HO-1) activity in the cavernous tissues of sildenafil citrate-treated rats. Asian J Androl 2007; 9: 377-81.
35. Rosano GM, Aversa A, Vitale C, Fabbri A, Fini M, et al. Chronic treatment with tadalafil improves endothelial function in men with increased cardiovascular risk. Eur Urol 2005; 47: 220-2.
36. Choi SM, Kim JE, Kang KK. Chronic treatment of DA-8159, a new phosphodiesterase type V inhibitor, attenuates endothelial dysfunction in stroke-prone spontaneously hypertensive rat. Life Sci 2006; 78: 1211-6.