A gram-negative bacterium producing a heat-stable nitrilase highly active on aliphatic dinitriles

Applied Microbiology and Biotechnology

Volumn 52, Issue 5, 1999, Pages 654-659

  Gavagan, J E ^a   DiCosimo, R ^a   Eisenberg, A ^a   Fager, S K ^a   Folsom, P W ^a   Hann, E C ^a   Schneider, K J ^b   Fallon, R D ^a  

^a DUPONT COMPANY   (United States)

^b Bio Technical Resources   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

NITRILASE; NITRILE;

ARTICLE; BACTERIAL GROWTH; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME STABILITY; GRAM NEGATIVE BACTERIUM; HEATING; NONHUMAN; THERMOSTABILITY;

ACIDOVORAX FACILIS;

EID: 0032699020     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002530051574     Document Type: Article

References (28)

1. Bandyopadhyay AK, Nagasawa T, Asano Y, Fujishiro K, Yoshiki T, Yamada H (1986) Purification and characterization of benzonitrilases from Arthrobacter sp. strain J1. Appl Environ Microbiol 51: 302-306
2. Bhalla TC, Aoshima M, Misawa S, Muramatsu R, Furuhashi K (1995) The molecular cloning and sequencing of the nitrilase gene of Rhodococcus rhodochrous PA-34. Acta Biotechnol 15: 297-306
3. Cramp R, Gilmour M, Cowan DA (1997) Novel thermophilic bacteria producing nitrile degrading enzymes. Microbiology 143: 2313-2320
4. Fallon RD, Stieglitz B, Turner I Jr (1997) A Pseudomonas putida capable of stereoselective hydrolysis of nitriles. Appl Microbiol Biotechnol 47: 156-161
5. Freyssinet G, Pelissier B, Freyssinet M, Delon R (1996) Crops resistant to oxynils: from the laboratory to the market. Field Crops Res 45: 125-133
6. Gavagan JE, Fager SK, Fallon RD, Folsom PW, Herkes FE, Eisenberg A, Hann EC, DiCosimo R (1998) Chemoenzymic production of lactams from aliphatic α,ω-dinitriles. J Org Chem 63: 4792-4801
7. Harper DB (1977a) Microbial metabolism of aromatic nitriles. Biochem J 165: 309-319
8. Harper DB (1977b) Fungal degradation of aromatic nitriles: enzymology of C-N cleavage by Fusarium solani. Biochem J 167: 685-692
9. Kobayashi M, Shimizu S (1994) Versatile nitrilase: Nitrile-hydrolyzing enzymes. FEMS Microbiol Lett 120: 217-224
10. Kobayashi M, Nagasawa T, Yamada H (1988) Regiospecific hydrolysis of dinitrile compounds by nitrilase from Rhodococcus rhodochrous J1. Appl Microbiol Biotechnol 29: 231-233
11. Kobayashi M, Yanaka N, Nagasawa T, Yamada H (1990a) Nitrilase catalyzed production of pyrazinoic acid, an antimycobacterial agent, from cyanopyrazine by resting cells of Rhodococcus rhodochrous J1. J Antibiot 43: 1316-1320
12. Kobayashi M, Yanaka N, Nagasawa T, Yamada H (1990b) Monohydrolysis of an aliphatic dinitrile compound by nitrilase from Rhodococcus rhodochrous K22. Tetrahedron 46: 5587-5590
13. Kobayashi M, Yanaka N, Nagasawa T, Yamada H (1991) Hyperinduction of an aliphatic nitrilase by Rhodococcus rhodochrous K22. FEMS Microbiol Lett 77: 121-124
14. Kobayashi M, Nagasawa T, Yamada H (1992) Enzymatic synthesis of acrylamide: a success story not yet over. Trends Biotechnol 10: 402-408
15. Levy-Schil S, Soubrier F, Crutz-Le Coq AM, Faucher D, Crouzet J, Petre D (1995) Aliphatic nitrilase from a soil-isolated Comamonas testosteroni sp.: gene cloning and overexpression, purification and primary structure. Gene 161: 15-20
16. Liu Z, Wang Y, Kounaves SP, Brush EJ (1993) Determination of organonitriles using enzyme-based selectivity mechanisms 1. An ammonia gas sensing electrode-based sensor for benzonitrile. Anal Chem 65: 3134-3136
17. McBride KE, Kenny JW, Stalker DM (1986) Metabolism of bromoxynil by Klebsiella pneumoniae subsp. ozaenae. Appl Environ Microbiol 52: 325-330
18. Nagasawa T, Kobayashi M, Yamada H (1988) Optimum culture conditions for the production of benzonitrilase by Rhodococcus rhodochrous J1. Arch Microbiol 150: 89-94
19. Nagasawa T, Nakamura T, Yamada H (1990) ε-caprolactam, a new powerful inducer for the formation of Rhodococcus rhodochrous J1 nitrilase. Arch Microbiol 155: 13-17
20. Nagasawa T, Shimizu S, Yamada H (1993) The superiority of the third generation catalyst Rhodococcus rhodochrous J1 nitrile hydratase for industrial production of acrylamide. Appl Microbiol Biotechnol 40: 189-195
21. Novo C, Tata R, Clemente A, Brown PR (1995) Pseudomonas aeruginosa aliphatic amidase is related to the nitrilase/cyanide hydratase enzyme family and Cys166 is predicted to be the active site nucleophile of the catalytic mechanism. FEBS Lett 367: 275-279
22. Payne MS, Wu S, Fallon RD, Tudor G, Stieglitz B, Turner IM Jr, Nelson MJ (1997) A stereoselective cobalt-containing nitrile hydratase. Biochemistry 36: 5447-5454
23. Sasser M, Smith DH (1987) Parallels between ribosomal RNA and DNA homologies and fatty acid composition in Pseudomonas. Abstr Am Soc Microbiol, p 135
24. Strickland JDH, Parsons TR (1972) A practical handbook of seawater analysis. Bull. 167, 2nd Edn. Fisheries Research Board of Canada, Ottawa
25. Veldkamp H (1970) Enrichment cultures of prokaryotic organisms. In: Norris, JR, Ribbons, DW (eds) Methods in microbiology, vol 3A. Academic Press, London, pp 305-361
26. Willems A, Falsen E, Pot B, Jantzen E, Hoste B, Vandamme P, Gillis M, Kersters K, De Ley J (1990) Acidovorax, a new genus for Pseudomonas facilis, Pseudomonas delafieldii, E. Falsen (EF) Group 13, EF Group 16, and several clinical isolates, with species Acidovorax facilis comb. nov., Acidovorax delafieldii comb. nov., and Acidovorax temperans sp. nov. Int J Syst Bacteriol 40: 384-398
27. Yamamoto K, Komatsu K (1991) Purification and characterization of nitrilase responsible for the enantioselective hydrolysis from Acinetobacter sp. AK 226. Agric Biol Chem 55: 1459-1466
28. Yamamoto K, Fujimatsu I, Komatsu K-I (1992) Purification and characterization of the nitrilase from Alcaligenesfaecalis ATCC 8750 responsible for enantioselective hydrolysis of mandelonitrile. J Ferm Bioeng 73: 425-430