Reverse micellar extraction for downstream processing of lipase: Effect of various parameters on extraction

Process Biochemistry

Volumn 44, Issue 10, 2009, Pages 1172-1178

  Nandini, K E ^a   Rastogi, Navin K ^a  

^a CENTRAL FOOD TECHNOLOGICAL RESEARCH INSTITUTE   (India)

Author keywords

Activity recovery; Cationic surfactant; Downstream processing; Extraction efficiency; Lipase; Reverse micellar extraction

Indexed keywords

ACTIVITY RECOVERY; AQUEOUS PHASE; BACK EXTRACTION; BACKWARD EXTRACTION; CATIONIC SURFACTANT CETYLTRIMETHYLAMMONIUM BROMIDES; CRUDE EXTRACT; DOWNSTREAM PROCESSING; EXTRACTION EFFICIENCIES; EXTRACTION EFFICIENCY; FORWARD-EXTRACTION; HEXANOL; MICELLAR EXTRACTION; ORGANIC PHASE; POTASSIUM PHOSPHATE; PROCESS PARAMETERS; PURIFICATION FACTORS; REVERSE MICELLAR EXTRACTION; TRIS BUFFERS;

ALCOHOLS; AMMONIUM COMPOUNDS; BIOPHYSICS; CATIONIC SURFACTANTS; PARAFFINS; POTASSIUM; SODIUM CHLORIDE; SOLVENT EXTRACTION;

DYES;

EID: 69249232046     PISSN: 13595113     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.procbio.2009.06.020     Document Type: Article

References (40)

1. Sharma R., Chisti Y., and Banerjee U.C. Production, purification, characterization and applications of lipases. Biotechnol Adv 19 (2001) 627-662
2. Shu C.H., Xu C.J., and Lin G.C. Purification and partial characterization of a lipase from Antrodia cinnamomea. Process Biochem 41 (2006) 734-738
3. Mathew D.S., and Juang R.S. Role of alcohols in the formation of inverse microemulsions and back extraction of proteins/enzymes in a reverse micellar system. Sep Purif Technol 53 (2007) 199-215
4. Shin Y.O., Wahnon D., Weber M.E., and Vera J.H. Selective precipitation and recovery of xylanase using surfactant and organic solvent. Biotechnol Bioeng 86 (2004) 698-705
5. Nandini K.E., and Rastogi N.K. Liquid-liquid extraction of lipase using aqueous two-phase system. Food Bioprocess Technol (2009) 10.1007/s11947-008-0160-0
6. Krishna S.H., Srinivas N.D., Raghavarao K.S.M.S., and Karanth N.G. Reverse micellar extraction for downstream processing of proteins/enzymes. In: Scheper T. (Ed). Advances in biochemical engineering/biotechnology 75 (2002), Springer-Verlag, Berlin 119-184
7. Chen Y.L., Su C.K., and Chiang B.H. Optimization of reversed micellar extraction of chitosanases produced by Bacillus cereus. Process Biochem 41 (2006) 752-758
8. Carvalho C.M.L., Barros M.R.A., and Cabral J.M.S. Cutinase: from molecular level to bioprocess development. Biotechnol Bioeng 66 (1999) 17-34
9. Goto M. Recent advances in protein extraction and chiral separation of biomolecules. Tsinghua Sci Technol 11 (2006) 194-201
10. Hagen A.J., Hatton T.A., and Wang D.I.C. Protein refolding in reversed micelles. Biotechnol Bioeng 95 (2006) 285-294
11. Dovyap Z., Bayraktar E., and Mehmetoglu U. Amino acid extraction and mass transfer rate in the reverse micelle system. Enzyme Microb Technol 38 (2006) 557-562
12. Wu J.C., Chau P.S., Chow Y., Li R., Carpenter K., and Yu H. Extraction of Candida rugosa lipase from aqueous solutions into organic solvents by forming an ion-paired complex wit AOT. J Chem Technol Biotechnol 81 (2006) 1003-1008
13. Yu Y.C., Chu Y., and Ji J.Y. Study of the factors affecting the forward and back extraction of yeast-lipase and its activity by reverse micelles. J Colloid Interf Sci 267 (2003) 60-64
14. Naoe K., Takeuchi C., Kawagoe M., Nagayama K., and Imai M. Higher order structure of Mucor miehei lipase and micelle size in cetyltrimethylammonium bromide reverse micellar system. J Chromatogr B 850 (2007) 277-284
15. Shen R., Liu J.G., Xing J.M., and Liu H.Z. Extraction of industrial lipase with CTAB-based mixed reverse micelles. Chin J Process Eng 5 (2005) 255-259
16. Mahadik N.D., Puntambekar U.S., Bastawde K.B., Khire J.M., and Gokhale D.V. Production of acidic lipase by Aspergillus niger in solid state fermentation. Process Biochem 38 (2002) 715-721
17. Bollang D.M., Rozycki M.D., and Edelstein S.J. Protein concentration determination. Protein methods. 2nd ed. (1996), John Wiley and Sons, New York p. 58-81
18. Macris J.B., Kourentzi E., and Hatzinikiolaou D.G. Studies on localization and regulation of lipase production by Aspergillus niger. Process Biochem 31 (1996) 807-812
19. Manning G.B., and Campbell L.L. Thermostable α-amylase of Bacillus stearothermophilus. J Biol Chem 236 (1961) 2952-2962
20. Greenberg D.M. Plant proteolytic enzyme. In: Colowick S.P., and Kaplan N.O. (Eds). Methods in enzymology (1995), Academic Press, London 54-64
21. Wood T.M., and Bhat K.M. Methods for measuring cellulase activities. In: Wood W.A., and Kellogg S.T. (Eds). Methods in enzymology (1982), Academic Press, London 87-113
22. Deak N.A., Murphy P.A., and Johnson L.A. Effects of NaCl concentration on salting-in and dilution during salting-out on soy protein fractionation. J Food Sci 71 (2006) C247-C254
23. Hebbar H.U., and Raghavarao K.S.M.S. Extraction of bovine serum albumin using nanoparticulate reverse micelles. Process Biochem 42 (2007) 1602-1608
24. Deuscher M. Methods in enzymology guide to protein purification; purification procedures. Electrophoretic Meth 182 (1990) 425-488
25. Hossain M.J., Hayashi T.Y., Kawanishi T., Shimizu N., and Nakamura R. Enzymatic activity of Chromobacterium viscosum lipase in an AOT/Tween 85 mixed reverse micellar system. J Chem Technol Biotechnol 74 (1999) 423-428
26. Tonova K., and Lazarova Z. Reversed micelle solvents as tools of enzyme purification and enzyme-catalyzed conversion. Biotechnol Adv 26 (2008) 516-532
27. Lazarova Z., and Tonova K. Integrated reverse micellar extraction and stripping of α-amylase. Biotechnol Bioeng 63 (1999) 583-592
28. Pessoa J.A., and Vitolo M. Recovery of insulinase using BDBAC reverse micelles. Process Biochem 33 (1998) 291-297
29. Hebbar H.U., Sumana B., and Raghavarao K.S.M.S. Use of reverse micellar systems for the extraction and purification of bromelain from pineapple wastes. Bioresour Technol 99 (2008) 4896-4902
30. Liu J.G., Xing J.M., Shen R., Yang C.L., and Liu H.Z. Reverse micelles extraction of nattokinase from fermentation broth. Biochem Eng J 21 (2004) 273-278
31. Noh K.H., and Imm J.Y. One-step separation of lysozyme by reverse micelles formed by the cationic surfactant, cetyldimethylammonium bromide. Food Chem 93 (2005) 95-101
32. Lesser M.E., Wei G., Luisi P.L., and Maestro M. Application of reverse micelles for the extraction of proteins. Biochem Biophys Res Commun 135 (1986) 629-635
33. Metzler D.E. Determining structures and analysing cells. Biochemistry, the chemical reactions of living cells (2001), Academic press, USA p. 95-149
34. Frutos C.M.E., Sonsoles P.V., Chiquinquira C., and Eduardo C. Reverse micelles in organic solvents: a medium for the biotechnological use of extreme halophilic enzyme at low salt concentration. Archaea 1 (2002) 105-111
35. Regalado C., Asenjo J.A., and Pyle D.L. Studies on the purification of peroxidase from horseradish roots using reverse micelles. Enzyme Microb Technol 18 (1996) 332-339
36. Gerhardt N.I., and Dungan S.R. Time-dependent solubilization of IgG in AOT-Brine isooctane microemulsions: role of cluster formation. Biotechnol Bioeng 78 (2002) 60-72
37. Tonova K., and Lazarova Z. Influence of enzyme aqueous source on RME-based purification of α-amylase. Sep Purif Technol 47 (2005) 43-51
38. Krei G., Meyer U., Borner B., and Hustedt H. Extraction of amylase using BDBAC-reversed micelles. Bioseparation 5 (1995) 175-183
39. Marcozzi G., Correa N., Luisi P.L., and Caselli M. Protein extraction by reverse micelles: a study of the factors affecting the forward and backward transfer of α-chymotrypsin and its activity. Biotechnol Bioeng 38 (1991) 1239-1246
40. Gaikar V.G., and Kulakarni M.S. Selective reverse micellar extraction of Penicillin acylase from E. coli. J Chem Technol Biotechnol 76 (2001) 729-736