Alterations in membrane potential in mitochondria isolated from brain subregions during focal cerebral ischemia and early reperfusion: Evaluation using flow cytometry

Neurochemical Research

Volumn 34, Issue 10, 2009, Pages 1857-1866

  Lee, Diane R ^a   Helps, Stephen C ^a   Macardle, Peter J ^a   Nilsson, Michael ^b   Sims, Neil R ^a  

^a FLINDERS UNIVERSITY   (Australia)

^b UNIVERSITY OF GOTHENBURG   (Sweden)

Author keywords

Flow cytometry; Focal ischemia; Mitochondria; Mitochondrial membrane potential; Reperfusion

Indexed keywords

RHODAMINE 123;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BRAIN CORTEX; BRAIN ISCHEMIA; BRAIN REGION; CELL DEATH; CLINICAL EVALUATION; CONTROLLED STUDY; CORPUS STRIATUM; ENERGY CONSUMPTION; FLOW CYTOMETRY; FLUORESCENCE; IN VITRO STUDY; MALE; MIDDLE CEREBRAL ARTERY OCCLUSION; MITOCHONDRIAL MEMBRANE POTENTIAL; MITOCHONDRION; NONHUMAN; PRIORITY JOURNAL; RAT; REPERFUSION;

ANIMALS; BRAIN; BRAIN ISCHEMIA; CELL SEPARATION; FLOW CYTOMETRY; INTRACELLULAR MEMBRANES; MALE; MEMBRANE POTENTIALS; MITOCHONDRIA; RATS; RATS, SPRAGUE-DAWLEY; REPERFUSION INJURY;

EID: 69049116118     PISSN: 03643190     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11064-009-0001-1     Document Type: Article

References (40)

1. NR Sims MF Anderson 2002 Mitochondrial contributions to tissue damage in stroke Neurochem Int 40 511 526 10.1016/S0197-0186(01)00122-X
2. L Hertz 2008 Bioenergetics of cerebral ischemia: a cellular perspective Neuropharmacology 55 289 309 10.1016/j.neuropharm.2008.05.023
3. J Folbergrova H Memezawa M-L Smith, et al. 1992 Focal and perifocal changes in tissue energy state during middle cerebral artery occlusion in normo- and hyperglycemic rats J Cereb Blood Flow Metab 12 25 33
4. J Folbergrova Q Zhao K Katsura, et al. 1995 N-tert-butyl-alpha- phenylnitrone improves recovery of brain energy state in rats following transient focal ischemia Proc Natl Acad Sci USA 92 5057 5061 10.1073/pnas.92.11.5057
5. S Kuroda K-I Katsura L Hillered, et al. 1996 Delayed treatment with α-phenyl-N-tert-butyl nitrone (PBN) attenuates secondary mitochondrial dysfunction after transient focal cerebral ischemia in the rat Neurobiol Dis 3 149 157 10.1006/nbdi.1996.0015
6. A Nakai S Kuroda A Kristián, et al. 1997 The immunosuppressant drug FK506 ameliorates secondary mitochondrial dysfunction following transient focal cerebral ischemia in the rat Neurobiol Dis 4 288 300 10.1006/nbdi.1997. 0146
7. MF Anderson NR Sims 1999 Mitochondrial respiratory function and cell death in focal cerebral ischemia J Neurochem 73 1189 1199 10.1046/j.1471-4159. 1999.0731189.x
8. JN Keller MS Kindy FW Holtsberg, et al. 1998 Mitochondrial manganese superoxide dismutase prevents neuronal apoptosis and reduces ischemic brain injury: suppression of peroxynitrite production, lipid peroxidation, and mitochondrial dysfunction J Neurosci 18 687 697
9. T Yoshimoto BK Siesjö 1999 Posttreatment with the immunosuppressant cyclosporin A in transient focal ischemia Brain Res 839 283 291 10.1016/S0006-8993(99)01733-3
10. S Matsumoto H Friberg M Ferrand-Drake, et al. 1999 Blockade of the mitochondrial permeability transition pore diminishes infarct size in the rat after transient middle cerebral artery occlusion J Cereb Blood Flow Metab 19 736 741 10.1097/00004647-199907000-00002
11. AS Korde LC Pettigrew SD Craddock, et al. 2007 Protective effects of NIM811 in transient focal cerebral ischemia suggest involvement of the mitochondrial permeability transition J Neurotrauma 24 895 908 10.1089/neu.2006.0122
12. G Kroemer L Galluzzi C Brenner 2007 Mitochondrial membrane permeability and cell death Physiol Rev 87 99 163 10.1152/physrev.00013.2006
13. A Schinzel O Takeuchi Z Huang, et al. 2005 Cyclophilin D is a component of mitochondrial permeability transition and mediates neuronal cell death after focal cerebral ischemia Proc Natl Acad Sci USA 102 12005 12010 10.1073/pnas.0505294102
14. Y Li M Chopp N Jiang, et al. 1995 Temporal profile of in situ DNA fragmentation after transient middle cerebral artery occlusion in the rat J Cereb Blood Flow Metab 15 389 397
15. M Asahi M Hoshimaru Y Uemura, et al. 1997 Expression of interleukin-1β converting enzyme gene family and bcl-2 gene family in the rat brain following permanent occlusion of the middle cerebral artery J Cereb Blood Flow Metab 17 11 18 10.1097/00004647-199701000-00003
16. M Fujimura Y Morita-Fujimura K Murakami 1998 Cytosolic redistribution of cytochrome c after transient focal cerebral ischemia in rats J Cereb Blood Flow Metab 18 1239 1247 10.1097/00004647-199811000-00010
17. S Namura JM Zhu K Fink, et al. 1998 Activation and cleavage of caspase-3 in apoptosis induced by experimental cerebral ischemia J Neurosci 18 3659 3668
18. Nicholls D, Ferguson SJ (2002) Bioenergetics 3. Academic Press, London, pp 3-8 and 57-66
19. J Duszynski K Boguka L Wojtczak 1984 Homeostasis of the protonmotive force in phosphorylating mitochondria Biochim Biophys Acta 767 540 547 10.1016/0005-2728(84)90053-7
20. RP Hafner GC Brown MD Brand 1990 Analysis of the control of the respiration rate, phosphorylation rate, proton leak rate and protonmotive force in isolated mitochondria using the 'top-down' approach of metabolic control theory Eur J Biochem 188 313 319 10.1111/j.1432-1033.1990.tb15405.x
21. L Piccotti C Marcetti G Migliorati, et al. 2002 Exogenous phospholipids specifically affect the transmembrane potential of brain mitochondria and cytochrome c release J Biol Chem 277 12075 12081 10.1074/jbc.M200029200
22. A Carlucci A Adornetto A Scorziello, et al. 2008 Proteolysis of AKAP121 regulates mitochondrial activity during cellular hypoxia and brain ischemia EMBO J 27 1073 1084 10.1038/emboj.2008.33
23. Vega-Nunez AM Alvarez A Menendez-Hurtado, et al. 1997 Neuronal mitochondrial morphology and transmembrane potential are severely altered by hypothyroidism during rat brain development Endocrinology 138 3771 3778 10.1210/en.138.9.3771
24. RK Emaus R Grunwald JJ Lemasters 1986 Rhodamine 123 as a probe of transmembrane potential in isolated rat-liver mitochondria: spectral and metabolic properties Biochim Biophys Acta 850 436 448 10.1016/0005-2728(86) 90112-X
25. PX Petit JE O'Connor D Grunwald SC Brown 1990 Analysis of the membrane potential of rat- and mouse-liver mitochondria by flow cytometry and possible applications Eur J Biochem 194 389 397 10.1111/j.1432-1033.1990.tb15632.x
26. RC Scaduto LW Grotyohann 1999 Measurement of mitochondrial membrane potential using fluorescent rhodamine derivatives Biophys J 76 469 477 10.1016/S0006-3495(99)77214-0
27. G Mattiasson H Friberg M Hansson E Elmer T Wieloch 2003 Flow cytometric analysis of mitochondria from CA1 and CA3 regions of rat hippocampus reveals differences in permeability transition pore activation J Neurochem 87 532 544 10.1046/j.1471-4159.2003.02026.x
28. 14C-acetate metabolism after temporary focal cerebral ischemia in rats J Cereb Blood Flow Metab 25 440 450 10.1038/sj.jcbfm.9600035
29. B Chance GR Williams 1956 The respiratory chain and oxidative phosphorylation Adv Enzymol Relat Subj Biochem 17 65 134
30. EZ Zea Longa PR Weinstein S Carlson, et al. 1989 Reversible middle cerebral artery occlusion without craniectomy in rats Stroke 20 84 91
31. NR Sims 1990 Rapid isolation of metabolically active mitochondria from rat brain and subregions using Percoll density gradient centrifugation J Neurochem 55 698 707 10.1111/j.1471-4159.1990.tb04189.x
32. NR Sims MF Anderson 2008 Isolation of mitochondria from rat brain using Percoll density gradient centrifugation Nat Protoc 3 1228 1239 10.1038/nprot.2008.105
33. A Cossarizza D Ceccarelli A Masini 1996 Functional heterogeneity of an isolated mitochondrial population revealed by cytofluorometric analysis at the single organelle level Exp Cell Res 222 84 94 10.1006/excr.1996.0011
34. OH Lowry NJ Rosebrough AL Farr, et al. 1951 Protein measurement with the Folin phenol reagent J Biol Chem 193 265 275
35. JW Phillis MH O'Regan 2004 A potentially critical role of phospholipases in central nervous system ischemic, traumatic, and neurodegenerative disorders Brain Res Brain Res Rev 44 13 47 10.1016/j.brainresrev.2003.10.002
36. M Ginsberg 2008 Neuroprotection for ischemic stroke Neuropharmacology 55 363 389 10.1016/j.neuropharm.2007.12.007
37. KP Doyle RP Simon MP Stenzel-Poore 2008 Mechanisms of ischemic brain damage Neuropharmacology 55 310 318 10.1016/j.neuropharm.2008.01.005
38. 14C-glucose by neurons and astrocytes in brain subregions following focal cerebral ischemia in rats J Neurochem 97 968 978 10.1111/j.1471-4159.2006. 03778.x
39. HG Bolander L Persson L Hillered R d'Argy U Ponten Y Olsson 1989 Regional cerebral blood flow and histopathologic changes after middle cerebral artery occlusion in rats Stroke 20 930 937
40. L Belayev W Zhao R Busto, et al. 1997 Transient middle cerebral artery occlusion by intraluminal suture: I. Three-dimensional autoradiographic image-analysis of local cerebral glucose metabolism-blood flow interrelationships during ischemia and early recirculation J Cereb Blood Flow Metab 17 1266 1280 10.1097/00004647-199712000-00002