Detecting protein-protein interactions in living cells: Development of a bioluminescence resonance energy transfer assay to evaluate the PSD-95/NMDA receptor interaction

Neurochemical Research

Volumn 34, Issue 10, 2009, Pages 1729-1737

  Gottschalk, Marie ^a   Bach, Anders ^a   Hansen, Jakob Lerche ^b   Krogsgaard Larsen, Povl ^a   Kristensen, Anders S ^a   Strømgaard, Kristian ^a  

^a UNIVERSITY OF COPENHAGEN   (Denmark)

^b UNIVERSITY OF COPENHAGEN   (Denmark)

Author keywords

BRET assay; BRET2; NMDA receptor; PDZ domain; Protein protein interactions; PSD 95

Indexed keywords

GREEN FLUORESCENT PROTEIN; N METHYL DEXTRO ASPARTIC ACID RECEPTOR; PDZ PROTEIN; POSTSYNAPTIC DENSITY PROTEIN 95; RENILLA LUCIFERIN 2 MONOOXYGENASE; TRANSACTIVATOR PROTEIN;

AMINO ACID SEQUENCE; ANIMAL CELL; ANIMAL CELL CULTURE; ARTICLE; BIOLUMINESCENCE RESONANCE ENERGY TRANSFER; CARBOXY TERMINAL SEQUENCE; CELL STRAIN COS7; CLINICAL EVALUATION; CONTROLLED STUDY; MEMBRANE PERMEABILITY; NONHUMAN; PDZ DOMAIN; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION;

AMINO ACID SEQUENCE; ANIMALS; CERCOPITHECUS AETHIOPS; COS CELLS; ENERGY TRANSFER; GENE PRODUCTS, TAT; GREEN FLUORESCENT PROTEINS; HUMANS; INTRACELLULAR SIGNALING PEPTIDES AND PROTEINS; LUCIFERASES, RENILLA; LUMINESCENT MEASUREMENTS; MEMBRANE PROTEINS; MOLECULAR SEQUENCE DATA; PROTEIN BINDING; PROTEIN INTERACTION DOMAINS AND MOTIFS; RECEPTORS, N-METHYL-D-ASPARTATE;

RENILLA LUCIFERASE;

EID: 69049086559     PISSN: 03643190     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11064-009-9998-4     Document Type: Article

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