Trypanosoma cruzi SHSP16: Characterization of an α-crystallin small heat shock protein

Experimental Parasitology

Volumn 123, Issue 2, 2009, Pages 182-189

  Pérez Morales, Deyanira ^a   Ostoa Saloma, Pedro ^a   Espinoza, Bertha ^a  

^a INSTITUTO DE INVESTIGACIONES BIOMÉDICAS   (Mexico)

Author keywords

Crystallin small heat shock protein; Heat shock; SHSP16; Trypanosoma cruzi

Indexed keywords

HEAT SHOCK PROTEIN; MALATE DEHYDROGENASE; SMALL HEAT SHOCK PROTEIN 16; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; CONTROLLED STUDY; GENE EXPRESSION; GENE IDENTIFICATION; GENETIC TRANSCRIPTION; HEAT STRESS; IN VITRO STUDY; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PRIORITY JOURNAL; PROTEIN AGGREGATION; PROTEIN DOMAIN; PROTEIN ISOLATION; PROTEOMICS; STRAIN DIFFERENCE; TRYPANOSOMA CRUZI;

ALPHA-CRYSTALLINS; AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; CLONING, MOLECULAR; CONSERVED SEQUENCE; DNA, PROTOZOAN; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GENE EXPRESSION; HEAT-SHOCK PROTEINS, SMALL; HOT TEMPERATURE; HUMANS; MOLECULAR SEQUENCE DATA; POLYMORPHISM, GENETIC; PROTEIN STRUCTURE, SECONDARY; PROTOZOAN PROTEINS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; RNA, PROTOZOAN; SEQUENCE ALIGNMENT; TRYPANOSOMA CRUZI;

TRYPANOSOMA CRUZI; TRYPANOSOMATIDAE;

EID: 68949169092     PISSN: 00144894     EISSN: 10902449     Source Type: Journal    
DOI: 10.1016/j.exppara.2009.06.019     Document Type: Article

References (52)

1. Abramoff M.D., Magelhaes P.J., and Ram S.J. Image processing with ImageJ. Biophoto. Int. 11 (2004) 36-42
2. Basha E., Lee G.J., Demeler B., and Vierling E. Chaperone activity of cytosolic small heat shock proteins from wheat. Eur. J. Biochem. 271 (2004) 1426-1436
3. Berriman M., Ghedin E., Hertz-Fowler C., et al. The genome of the African trypanosome Trypanosoma brucei. Science 309 (2005) 416-422
4. Bosseno M.F., Barnabé C., Magallón Gastélum E., Lozano Kasten F., Ramsey J., Espinoza B., and Brenière S.F. Predominance of Trypanosoma cruzi Lineage I in Mexico. J. Clin. Microbiol. 40 (2002) 627-632
5. Cevallos A.M., Pérez-Escobar M., Espinosa N., Herrera J., López-Villaseñor I., and Hernández R. The stabilization of housekeeping transcripts in Trypanosoma cruzi epimastigotes evidences a global regulation of RNA decay during stationary phase. FEMS Microbiol. Lett. 246 (2005) 259-264
6. Clayton C., Adams M., Almeida R., et al. Genetic nomenclature for Trypanosoma and Leishmania. Mol. Biochem. Parasitol. 97 (1998) 221-224
7. de Jong W.W., Caspers G.J., and Leunissen J.A.M. Genealogy of the alpha-crystallin-small heat-shock protein superfamily. Int. J. Biol. Macromol. 22 (1998) 151-162
8. de Miguel N., Echeverria P.C., and Angel S.O. Differential subcellular localization of members of the Toxoplasma gondii small heat shock protein family. Eukaryot. Cell 4 (2005) 1990-1997
9. De Souza W. Basic cell biology of Trypanosoma cruzi. Curr. Pharm. Des. 8 (2002) 269-285
10. Dos Santos W.G., and Buck G.A. Polymorphisms at the topoisomerase II gene locus provide more evidence for the partition of Trypanosoma cruzi into two major groups. J. Eukaryot. Microbiol. 46 (1999) 17-23
11. Dragon E.A., Sias S.R., Kato E.A., and Gabe J.D. The genome of Trypanosoma cruzi contains a constitutively expressed, tandemly arranged multicopy gene homologous to a major heat shock protein. Mol. Cell. Biol. 7 (1987) 1271-1275
12. El-Sayed N.M., Myler P.J., Bartholomeu D.C., et al. The genome sequence of Trypanosoma cruzi, etiologic agent of Chagas disease. Science 309 (2005) 409-415
13. Espinoza B., Vera-Cruz J.M., González H., Ortega E., and Hernández R. Genotype and virulence correlation within Mexican stocks of Trypanosoma cruzi isolated from patients. Acta Trop. 15 (1998) 63-72
14. Fernandes M., Silva R., Rössle S.C., Bisch P.M., Rondinelli E., and Ürményi T.P. Gene characterization and predicted protein structure of the mitochondrial chaperonin HSP10 of Trypanosoma cruzi. Gene 349 (2005) 135-142
15. Ferrer E., González L.M., Foster-Cuevas M., Cortéz M.M., Dávila I., Rodríguez M., Sciutto E., Harrison L.J.S., Parkhouse R.M.E., and Gárate T. Taenia solium: characterization of a small heat shock protein (Tsol-sHSP35.6) and its possible relevance to the diagnosis and pathogenesis of neurocysticercosis. Exp. Parasitol. 110 (2005) 1-11
16. Fu X., and Chang Z. Identification of a highly conserved Pro-Gly doublet in non-animal small heat shock proteins and characterization of its structural and functional roles in Mycobacterium tuberculosis Hsp16.3. Biochemistry (Moscow) 71 Suppl. 1 (2006) S83-S90
17. Giambiagi-de Marval M., Gottesdiener K., Rondinelli E., and Van Der Ploeg L.H.T. Predicted amino acid sequence and genomic organization of Trypanosoma cruzi hsp 60 genes. Mol. Biochem. Parasitol. 58 (1993) 25-32
18. Giambiagi-de Marval M., Souto-Padrón T., and Rondinelli E. Characterization and cellular distribution of heat-shock proteins HSP70 and HSP60 in Trypanosoma cruzi. Exp. Parasitol. 83 (1996) 335-345
19. González-Márquez H., Perrin C., Bracquart P., Guimont C., and Linden G. A 16 kDa protein family overexpressed by Streptococcus thermophilus PB18 in acid environments. Microbiology 143 (1997) 1587-1594
20. Graefe S.E.B., Wiesgigl M., Gaworski I., Macdonald A., and Clos J. Inhibition of HSP90 in Trypanosoma cruzi induces a stress response but no stage differentiation. Eukaryot. Cell 1 (2002) 936-943
21. Haslbeck M. sHsps and their role in the chaperone network. Cell. Mol. Life Sci. 59 (2002) 1649-1657
22. Horwitz J. Α-crystallin can function as a molecular chaperone. Proc. Natl. Acad. Sci. USA 89 (1992) 10449-10453
23. Ivens A.C., Peacock C.S., Worthey E.A., et al. The genome of the kinetoplastid parasite, Leishmania major. Science 309 (2005) 436-442
24. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227 (1970) 680-685
25. Lee G.J., Roseman A.M., Saibil H.R., and Vierling E. A small heat shock protein stably binds heat-denatured model substrates and can maintain a substrate in a folding-competent state. EMBO J. 16 (1997) 659-671
26. Lindquist S., and Craig E.A. The heat-shock proteins. Annu. Rev. Genet. 22 (1988) 631-677
27. López-Olmos V., Pérez-Nasser N., Piñero D., Ortega E., Hernández R., and Espinoza B. Biological characterization and genetic diversity of Mexican isolates of Trypanosoma cruzi. Acta Trop. 69 (1998) 239-254
28. Macedo A.M., Martins M.S., Chiari E., and Pena S.D. DNA fingerprinting of Trypanosoma cruzi: a new tool for characterization of strains and clones. Mol. Biochem. Parasitol. 55 (1992) 147-153
29. Machado C.A., and Ayala F.J. Sequence variation in the dihydrofolate reductase-thymidylate synthase (DHFR-TS) and trypanothione reductase (TR) genes of Trypanosoma cruzi. Mol. Biochem. Parasitol. 121 (2002) 33-47
30. MacRae T.H. Structure and function of small heat shock/alpha-crystallin proteins: established concepts and emerging ideas. Cell. Mol. Life Sci. 57 (2000) 899-913
31. Marchler-Bauer A., and Bryant S.H. CD-Search: protein domain annotations on the fly. Nucleic Acids Res. 32 W (2004) 327-331
32. Montalvo-Álvarez, A.M., Folguiera, C., Carrión, J., Monzote-Fidalgo, L., Cañavate, C., Requena, J.M. 2008. The Leishmania HSP20 is antigenic during natural infections, but, as DNA vaccine, it does protect BALB/c mice against experimental L. amazonensis infection. J. Biomed. Biotechnol. 2008, 1-9.
33. Narberhaus F. Alpha-crystallin-type heat shock proteins: socializing minichaperones in the context of a multichaperone network. Microbiol. Mol. Biol. Rev. 66 (2002) 64-93
34. Olson C.L., Nadeau K.C., Sullivan M.A., Winquist A.G., Donelson J.E., Walsh C.T., and Engman D.M. Molecular and biochemical comparison of the 70-kDa heat shock proteins of Trypanosoma cruzi. J. Biol. Chem. 269 (1994) 3868-3874
35. Ovali M., and King R.D. Cascaded multiple classifiers for secondary structure prediction. Prot. Sci. 9 (2000) 1162-1176
36. Pérez-Escobar M., Cevallos A.M., Espinoza B., Espinosa N., Martínez I., and Hernández R. Trypanosoma cruzi ribosomal protein S4: characterization of its coding locus, analysis of transcripts, and antigenicity of the protein. Mem. Inst. Oswaldo Cruz 102 (2007) 473-479
37. Rasband, W.S., 1997-2008. ImageJ. US National Institutes of Health, Bethesda, Maryland, USA. Available from: .
38. Reineke A. Identification and expression of a small heat shock protein in two lines of the endoparasitic wasp Venturia canescens. Comp. Biochem. Physiol. A Mol. Integr. Physiol. 141 (2005) 60-69
39. Requena J.M., López M.C., Jimenez-Ruiz A., de la Torre J.C., and Alonso C. A head-to-tail tandem organization of hsp70 genes in Trypanosoma cruzi. Nucleic Acids Res. 16 (1988) 1393-1406
40. Rogalla T., Ehrnsperger M., Preville X., Kotlyarov A., Lutsch G., Ducasse C., Paul C., Wieske M., Arrigo A., Buchner J., and Gaestel M. Regulation of hsp27 oligomerization, chaperone function, and protective activity against oxidative stress/tumor necrosis factor α by phosphorylation. J. Biol. Chem. 274 (1999) 18947-18956
41. Sambrook J., Fritsch E.F., and Maniatis T. Molecular Cloning: A Laboratory Manual. second ed. (1989), Cold Spring Harbor Laboratory Press, Cold Spring Harbor
42. Schofield C.J., Jannin J., and Salvatella R. The future of Chagas disease control. Trends Parasitol. 22 (2006) 583-588
43. Souto R.P., Fernandes O., Macedo A.M., Campbell D.A., and Zingales B. DNA markers define two major phylogenetic lineages of Trypanosoma cruzi. Mol. Biochem. Parasitol. 83 (1996) 141-152
44. Spano G., Beneduce L., Perrota C., and Massa S. Cloning and characterization of the hsp 18.55 gene, a new member of the small heat shock gene family isolated from wine Lactobacillus plantarum. Res. Microbiol. 156 (2005) 219-224
45. Studer S., Obrist M., Lentze N., and Narberhaus F. A critical motif for oligomerization and chaperone activity of bacterial alpha-heat shock proteins. Eur. J. Biochem. 269 (2002) 3578-3586
46. Sturm N.R., Vargas N.S., Westenberger S.J., Zingales B., and Campbell D.A. Evidence for multiple hybrid groups in Trypanosoma cruzi. Int. J. Parasitol. 33 (2003) 269-279
47. Tibayrenc M. Population genetics of parasitic protozoa and other microorganisms. Adv. Parasitol. 36 (1995) 47-115
48. Tibbetts R.S., Jensen J.L., Olson C.L., Wang F.D., and Engman D.M. The DnaJ family of protein chaperones in Trypanosoma cruzi. Mol. Biochem. Parasitol. 91 (1998) 319-326
49. van Montfort R.L.M., Basha E., Friedrich K.L., Slingsby C., and Vierling E. Crystal structure and assembly of a eukaryotic small heat shock protein. Nat. Struct. Biol. 8 (2001) 1025-1030
50. Westenberger S.J., Barnabé C., Campbell D.A., and Sturm N.R. Two hybridization events define the population structure of Trypanosoma cruzi. Genetics 171 (2005) 527-543
51. WHO - World Health Organization, 2002. Control of Chagas Disease. WHO Technical Report Series, vol. 905, pp. 82-83.
52. Wu Z., Nagano I., Boonmars T., and Takahashi Y. Thermally induced and developmentally regulated expression of a small heat shock protein in Trichinella spiralis. Parasitol. Res. 101 (2007) 201-212