Synergic effects of α-tocopherol and β-carotene on tert-butylhydroperoxide-induced HepG2 cell injury

Toxicology and Industrial Health

Volumn 25, Issue 5, 2009, Pages 311-320

  Park, S ^a   Kim, Aj ^b   Lee, M ^c  

^a Semyung University   (South Korea)

^b Hyejeon University   (South Korea)

^c Sungshin Women's University   (South Korea)

Author keywords

CYP2E1; DNA adduction; HepG2; tocopherol; carotene

Indexed keywords

AFLATOXIN B1; ALCOHOL; ALPHA TOCOPHEROL; BETA CAROTENE; BIOLOGICAL MARKER; CYTOCHROME P450 2E1; DNA; LACTATE DEHYDROGENASE; MALONALDEHYDE; TERT BUTYL HYDROPEROXIDE; THIOBARBITURIC ACID REACTIVE SUBSTANCE;

ANTIOXIDANT ACTIVITY; CELL DAMAGE; CELL DEATH; CELL DISRUPTION; CELL PROLIFERATION; CELL PROTECTION; CELL STRESS; CONCENTRATION RESPONSE; CONFERENCE PAPER; CONTROLLED STUDY; DNA ADDUCT; DNA DAMAGE; DRUG CYTOTOXICITY; DRUG EFFECT; DRUG MECHANISM; DRUG POTENTIATION; ENZYME REGULATION; HEPATOMA CELL; HUMAN; HUMAN CELL; LIPID PEROXIDATION; LIVER PROTECTION; OXIDATIVE STRESS;

AFLATOXIN B1; ALPHA-TOCOPHEROL; ANTIOXIDANTS; BETA CAROTENE; CELL LINE, TUMOR; CELL PROLIFERATION; CYTOCHROME P-450 CYP2E1; CYTOPROTECTION; DNA ADDUCTS; DOSE-RESPONSE RELATIONSHIP, DRUG; DRUG SYNERGISM; HUMANS; L-LACTATE DEHYDROGENASE; LIPID PEROXIDATION; OXIDATIVE STRESS; TERT-BUTYLHYDROPEROXIDE; VITAMINS;

EID: 68849110158     PISSN: 07482337     EISSN: None     Source Type: Journal    
DOI: 10.1177/0748233709106443     Document Type: Article

References (37)

1. Alia M, Sonia Ramos S, Mateos R, Bravo L, Goya L (2005) Response of the antioxidant defense system to tert-butyl hydroperoxide and hydrogen peroxide in a human hepatoma cell line (HepG2). J Biochem Mol Toxicol 19: 119–128.
2. Blomhoff R (2005) Dietary antioxidants and cardiovascular disease. Curr Opin Lipidol 16: 47–54.
3. Chen HW, Chiang T, Wang CY, Lii CK (2000) Inhibition of tert-butyl hydroperoxide-induced cell membrane bleb formation by alpha-tocopherol and glutathione. Food Chem Toxicol 38: 1089–1096.
4. Frei B (1994) Reactive oxygen species and antioxidant vitamins: mechanisms of action. Am J Med 97: 5S–13S.
5. Glascott PA, Gilfor E, Farber JL (1992) Effects of vitamin E on the killing of cultured hepatocytes by tert-butyl hydroperoxide. Mol Pharmacol 41: 1155–1162.
6. Glascott PA, Gilfor E, Farber JL (1995) Relationship of the metabolism of vitamins C and E in cultured hepatocytes treated with tert-butyl hydroperoxide. Mol Pharmacol 48: 80–88.
7. Guengerich FP, Kim DH, Iwasaki M (1991) Role of human cytochrome P-450 IIE1 in the oxidation of many low molecular weight cancer suspects. Chem Res Toxicol 4: 168–179.
8. Guiardelli A, Cattabeni F, Cantoni A (1997) Alternative mechanisms for hydroperoxide-induced DNA single strand breakage. Free Radic Res 26: 537–547.
9. Gumpricht E, Dahl R, Devereaux MW, Sokol RJ (2004) Beta-carotene prevents bile acid-induced cytotoxicity in the rat hepatocyte: Evidence for an antioxidant and anti-apoptotic role of beta-carotene in vitro. Pediatr Res 55: 814–821.
10. Hosotani K, Kawahata A, Koyama K, Murakami C, Yoshida H, Yamaji R (2004) Effect of carotenoids and ascorbic acid of Japanese persimmons on cellular lipid peroxidation in HepG2 cells. Biofactors 21: 241–245.
11. Imlay JM, Linn S (1988) DNA damage and oxygen radical toxicity. Science 240: 1302–1309.
12. Jimenez-Lopez JM, Cederbaum AI (2004) Green tea polyphenol epigallocatechin-3-gallate protects HepG2 cells against CYP2E1-dependent toxicity. Free Radic Biol Med 36: 359–370.
13. Kawanishi S, Oikawa S, Murata M (2005) Evaluation for safety of antioxidant chemopreventive agents. Antioxid Redox Signal 7: 1728–1739.
14. Koop DR (1992) Oxidative and reductive metabolism by cytochrome P450 2E1. FASEB J 6: 724–730.
15. Lee M, Bae MA (2007) Docosahexaenoic acid induces apoptosis in CYP2E1-containing HepG2 cells by activating the c-Jun N-terminal protein kinase related mitochondrial damage. J Nutr Biochem 18: 348–354.
16. Liu RH (2004) Potential synergy of phytochemicals in cancer prevention: mechanism of action. J Nutr 134: 3479S–3485S.
17. Mace K, Aguilar F, Wang JS, Vautravers P, Gómez-Lechón M, Gonzalez FJ (1997) Aflatoxin B1-induced DNA adduct formation and p53 mutation in CYP450-expressing human liver cell lines. Carcinogenesis 18: 1291–1297.
18. Mari M, Cederbaum AI (2001) Induction of catalase, alpha and microsomal glutathione S-transferase in CYP2E1-overexpressing HepG2 cells and protection against short-term oxidative stress. Hepatology 33: 652–661.
19. Martin C, Martinez R, Navarro R, Ruiz-Sanz JI, Lacort M, Ruiz-Larrea MB (2001) tert-Butyl hydroperoxide-induced lipid signaling in hepatocytes: involvement of glutathione and free radicals. Biochem Pharmacol 62: 705–712.
20. Martin KR, Failla ML, Smith JC (1996) Beta-carotene and lutein protect HepG2 human liver cells against oxidant-induced damage. J Nutr 126: 2098–2106.
21. Meydani M (2004) Vitamin E modulation of cardiovascular disease. Ann N Y Acad Sci 1031: 271–279.
22. Mihara M, Uchiyama M (1983) Effects of antioxidants on the TBA reaction of various rat liver homogenates. Biochem Med 30: 131–134.
23. Naito Y, Shimozawa M, Kuroda M, Nakabe N, Manabe H, Katada K (2005) Tocotrienols reduce 25-hydroxycholesterol-induced monocyte-endothelial cell interaction by inhibiting the surface expression of adhesion molecules. Atherosclerosis 180: 19–25.
24. Nordmann R, Ribiere C, Rouach H (1992) Implication of free radical mechanisms in ethanol-induced cellular injury. Free Radic Biol Med 12: 219–240.
25. Paterson E, Gordon MH, Niwat C, George TW, Parr L, Waroonphan S, Lovegrove JA (2006) Supplementation with fruit and vegetable soups and beverages increases plasma carotenoid concentrations but does not alter markers of oxidative stress or cardiovascular risk factors. J Nutr 136: 2849–2855.
26. Robertson G, Leclercq I, Farrell GC (2001) Nonalcoholic steatosis and steatohepatitis II. Cytochrome P-450 enzymes and oxidative stress. Am J Physiol Gastrointest Liver Physiol 281: 1135–1139.
27. Sharma S, Stutzman JD, Kelloff GJ, Steele VE (1994) Screening of potential chemopreventive agents using biochemical markers of carcinogenesis. Cancer Res 54: 5848–5855.
28. Sies H (1997) Antioxidant in Disease Mechanisms and Therapy Advances in Pharmacology. Academic press: SanDiego, USA. p. 320.
29. Silvers KJ, Couch LH, Rorke EA, Howanrd PC (1997) Role of nitroreductases but not cytochrome P450 in the metabolic activation of 1-Nitropyrene in the HepG2 human hepatoblastoma cell line. Biochem Pharmacol 54: 927–936.
30. Thurman RG (1973) Induction of hepatic microsomal reduced nicotinamide adenine dinucleotide phosphate-dependent production of hydrogen peroxide by chronic prior treatment with ethanol. Mol Pharmacol 9: 670–675.
31. Tirmenstein MA, Gie X, Elkins CA, Fariss MW (1999) Administration of the tris salt of tocopheryl hemisuccinate inactivates CYP2E1, enhances microsomal tocopherol levels and protects against carbon tetrachiloride-induced hepatoxicity. Free Radic Biol Med 26: 825–835.
32. Trombino S, Serini S, Di Nicuolo F, Celleno L, Andò S, Picci N (2004) Antioxidant effect of ferulic acid in isolated membranes and intact cells: synergistic interactions with alpha-tocopherol, beta-carotene, and ascorbic acid. J Agric Food Chem 52: 2411–2420.
33. Woods JA, Bilton RF, Young AJ (1999) β-Carotene enhances hydrogen peroxide-induced DNA damage in human hepatocellular HepG2 cells. FEBS Lett 449: 255–258.
34. Wu D, Cederbaum AI (2005) Oxidative stress mediated toxicity exerted by ethanol-inducible CYP2E1. Toxicol Appl Pharmacol 207: 70–76.
35. Xu Y, Leo MA, Lieber CS (2003) Lycopene attenuates arachidonic acid toxicity in HepG2 cells overexpressing CYP2E1. Biochem Biophys Res Commun 303: 745–750.
36. Yagi K, Ohtsuka K, Ohishi N (1985) Lipid peroxidation caused by chinoform-ferric chelate in cultured neural retinal cells. Experientia 41: 1561–1563.
37. Zima T, Kalousová M (2005) Oxidative stress and signal transduction pathways in alcoholic liver disease. Alcohol Clin Exp Res 29: 110S–115S.