A mechanism for incorporation of galectin-3 into the spliceosome through its association with U1 snRNP

Biochemistry

Volumn 48, Issue 32, 2009, Pages 7705-7712

  Haudek, Kevin C ^a   Voss, Patricia G ^a   Locascio, Lauren E ^a   Wang, John L ^a   Patterson, Ronald J ^b  

^a Michigan State University   (United States)

^b NONE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GALECTIN-1; IMMUNOPRECIPITATIONS; NUCLEAR EXTRACTS; PRE-MRNA; PRE-TREATMENT; SPLICE SITE; SPLICEOSOMES; SPLICING FACTORS;

GLYCEROL; NUCLEIC ACIDS; POLYPEPTIDES; PRECIPITATION (CHEMICAL); RNA; SUGARS;

SUBSTRATES;

GALECTIN 3; GLYCEROL; LACTOSE; LIGAND; MESSENGER RNA PRECURSOR; POLYPEPTIDE; RIBONUCLEASE A; SMALL NUCLEAR RIBONUCLEOPROTEIN;

ARTICLE; CELL NUCLEUS; COMPLEX FORMATION; CONTROLLED STUDY; FRACTIONATION; HELA CELL; HUMAN; HUMAN CELL; IMMUNOPRECIPITATION; MOLECULAR MECHANICS; PRECIPITATION; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN LOCALIZATION; RNA SPLICING; SEDIMENTATION; SPLICEOSOME;

ANIMALS; GALECTIN 3; HELA CELLS; HUMANS; MACROMOLECULAR SUBSTANCES; RIBONUCLEOPROTEIN, U1 SMALL NUCLEAR; RNA PRECURSORS; RNA SPLICING; SPLICEOSOMES;

EID: 68849093295     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi900071b     Document Type: Article

References (31)

1. Rappsilber, J., Ryder, U., Lamond, A. I., and Mann, M. (2002) Large-scale proteomic analysis of the human spliceosome. Genome Res. 12, 1231-1245.
2. Zhou, Z., Licklider, L. J., Gygi, S. P., and Reed, R. (2002) Comprehensive proteomic analysis of the human spliceosome. Nature 419, 182-185.
3. Jurica, M. S., Licklider, L. J., Gygi, S. R., Grigorieff, N., and Moore, M. J. (2002) Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis. RNA 8, 426-439.
4. Hartmuth, K., Urlaub, H., Vornlocher, H. P., Will, C. L., Gentzel, M., Wilm, M., and Luhrmann, R. (2002) Protein composition of human prespliceosomes isolated by a tobramycin affinity-selection method. Proc. Natl. Acad. Sci. U.S.A. 99, 16719-16724.
5. Brow, D. A. (2002) Allosteric cascade of spliceosome activation. Annu. Rev. Genet. 36, 333-360.
6. Michaud, S., and Reed, R. (1991) An ATP-independent complex commits pre-mRNA to the mammalian spliceosome assembly pathway. Genes Dev. 5, 2534-2546.
7. Barondes, S. H., Castronovo, V., Cooper, D. N., Cummings, R. D., Drickamer, K., Feizi, T., Gitt, M. A., Hirabayashi, J., Hughes, C., and Kasai, K..; et al. (1994) Galectins: A family of animal β-galactoside-binding lectins. Cell 76, 597-598.
8. Dagher, S. F., Wang, J. L., and Patterson, R. J. (1995) Identification of galectin-3 as a factor in pre-mRNA splicing. Proc. Natl. Acad. Sci. U.S.A. 92, 1213-1217.
9. Vyakarnam, A., Dagher, S. F., Wang, J. L., and Patterson, R. J. (1997) Evidence for a role for galectin-1 in pre-mRNA splicing. Mol. Cell. Biol. 17, 4730-4737.
10. Wang, W., Park, J. W., Wang, J. L., and Patterson, R. J. (2006) Immunoprecipitation of spliceosomal RNAs by antisera to galectin-1 and galectin-3. Nucleic Acids Res. 34, 5166-5174.
11. Dignam, J. D., Lebovitz, R. M., and Roeder, R. G. (1983) Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei. Nucleic Acids Res. 11, 1475-1489.
12. Agrwal, N., Sun, Q., Wang, S. Y., and Wang, J. L. (1993) Carbohydrate-binding protein 35. I. Properties of the recombinant polypeptide and the individuality of the domains. J. Biol. Chem. 268, 14932-14939.
13. Ho, M. K., and Springer, T. A. (1982) Mac-2, a novel 32,000 Mr mouse macrophage subpopulation-specific antigen defined by monoclonal antibodies. J. Immunol. 128, 1221-1228.
14. Lamond, A. I., Sproat, B., Ryder, U., and Hamm, J. (1989) Probing the structure and function of U2 snRNP with antisense oligonucleotides made of 2′-OMe RNA. Cell 58, 383-390.
15. Zillmann, M., Zapp, M. L., and Berget, S. M. (1988) Gel electrophoretic isolation of splicing complexes containing U1 small nuclear ribonucleoprotein particles. Mol. Cell. Biol. 8, 814-821.
16. Zillmann, M., Rose, S. D., and Berget, S. M. (1987) U1 small nuclear ribonucleoproteins are required early during spliceosome assembly. Mol. Cell. Biol. 7, 2877-2883.
17. Melton, D. A., Krieg, P. A., Rebagliati, M. R., Maniatis, T., Zinn, K., and Green, M. R. (1984) Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter. Nucleic Acids Res. 12, 7035-7056.
18. Park, J. W., Voss, P. G., Grabski, S., Wang, J. L., and Patterson, R. J. (2001) Association of galectin-1 and galectin-3 with Gemin4 in complexes containing the SMN protein. Nucleic Acids Res. 29, 3595-3602.
19. Conway, G. C., Krainer, A. R., Spector, D. L., and Roberts, R. J. (1989) Multiple splicing factors are released from endogenous complexes during in vitro pre-mRNA splicing. Mol. Cell. Biol. 9, 5273-5280.
20. Peng, R., Hawkins, I., Link, A. J., and Patton, J. G. (2006) The splicing factor PSF is part of a large complex that assembles in the absence of pre-mRNA and contains all five snRNPs. RNA Biol. 3, 69-76.
21. Voss, P. G., Gray, R. M., Dickey, S. W., Wang, W., Park, J. W., Kasai, K., Hirabayashi, J., Patterson, R. J., and Wang, J. L. (2008) Dissociation of the carbohydrate-binding and splicing activities of galectin-1. Arch. Biochem. Biophys. 478, 18-25.
22. Stevens, S. W., Ryan, D. E., Ge, H. Y., Moore, R. E., Young, M. K., Lee, T. D., and Abelson, J. (2002) Composition and functional characterization of the yeast spliceosomal penta-snRNP. Mol. Cell 9, 31-44.
23. Bottner, C. A., Schmidt, H., Vogel, S., Michele, M., and Kaufer, N. F. (2005) Multiple genetic and biochemical interactions of Brr2, Prp8, Prp31, Prp1 and Prp4 kinase suggest a function in the control of the activation of spliceosomes in Schizosaccharomyces pombe. Curr. Genet. 48, 151-161.
24. Malca, H., Shomron, N., and Ast, G. (2003) The U1 snRNP base pairs with the 5′ splice site within a penta-snRNP complex. Mol. Cell. Biol. 23, 3442-3455.
25. Patton, J. R., Patterson, R. J., and Pederson, T. (1987) Reconstitution of the U1 small nuclear ribonucleoprotein particle. Mol. Cell. Biol. 7, 4030-4037.
26. Bindereif, A., and Green, M. R. (1987) An ordered pathway of snRNP binding during mammalian pre-mRNA splicing complex assembly. EMBO J. 6, 2415-2424.
27. Mount, S. M., Pettersson, I., Hinterberger, M., Karmas, A., and Steitz, J. A. (1983) The U1 small nuclear RNA-protein complex selectively binds a 5′ splice site in vitro. Cell 33, 509-518.
28. Wang, M. J., and Gegenheimer, P. (1990) Substrate masking: Binding of RNA by EGTA-inactivated micrococcal nuclease results in artifactual inhibition of RNA processing reactions. Nucleic Acids Res. 18, 6625-6631.
29. Hinterberger, M., Pettersson, I., and Steitz, J. A. (1983) Isolation of small nuclear ribonucleoproteins containing U1, U2, U4, U5, and U6 RNAs. J. Biol. Chem. 258, 2604-2613.
30. Bringmann, P., and Luhrmann, R. (1986) Purification of the individual snRNPs U1, U2, U5 and U4/U6 from HeLa cells and characterization of their protein constituents. EMBO J. 5, 3509-3516.
31. Pettersson, I., Hinterberger, M., Mimori, T., Gottlieb, E., and Steitz, J. A. (1984) The structure of mammalian small nuclear ribonucleoproteins. Identification of multiple protein components reactive with anti-(U1) ribonucleoprotein and anti-Sm autoantibodies. J. Biol. Chem. 259, 5907-5914.