Quantitative isotopic 13C nuclear magnetic resonance at natural abundance to probe enzyme reaction mechanisms via site-specific isotope fractionation: The case of the chain-shortening reaction for the bioconversion of ferulic acid to vanillin

Analytical Biochemistry

Volumn 393, Issue 2, 2009, Pages 182-188

  Botosoa, Eliot P ^a   Blumenstein, Christine ^a   MacKenzie, Donald A ^b   Silvestre, Virginie ^a   Remaud, Gérald S ^a   Kwiecień, Renata A ^a   Robins, Richard J ^a  

^a UNIVERSITÉ DE NANTES   (France)

^b NORWICH RESEARCH PARK   (United Kingdom)

Author keywords

Biotransformation; Chain shortening reaction; Ferulic acid; Isotopic 13C NMR spectrometry; Reaction mechanisms; Streptomyces; Vanillin

Indexed keywords

BACTERIA; BIOCONVERSION; ENZYMES; ISOTOPES; QUINONE; REACTION INTERMEDIATES; SPECTROMETRY;

13C NMR; BIOTRANSFORMATION; CHAIN SHORTENING; FERULIC ACIDS; REACTION MECHANISM; STREPTOMYCES; VANILLIN;

NUCLEAR MAGNETIC RESONANCE;

FERULIC ACID; HYDROLYASE; LYASE; VANILLIN; 4 HYDROXYCINNAMOYL COA HYDRATASE LYASE; 4-HYDROXYCINNAMOYL-COA HYDRATASE-LYASE; BENZALDEHYDE DERIVATIVE; CARBON; COUMARIC ACID;

ACCURACY; ARTICLE; CARBON NUCLEAR MAGNETIC RESONANCE; CATABOLISM; CONTROLLED STUDY; ENZYME MECHANISM; FRACTIONATION; MOLECULAR PROBE; NONHUMAN; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; SENSITIVITY ANALYSIS; STREPTOMYCES; ANALYSIS; CHEMISTRY; ENZYMOLOGY; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY;

STREPTOMYCES; STREPTOMYCES SETONII;

ANALYTIC SAMPLE PREPARATION METHODS; BENZALDEHYDES; CARBON ISOTOPES; COUMARIC ACIDS; HYDRO-LYASES; MAGNETIC RESONANCE SPECTROSCOPY; STREPTOMYCES;

EID: 68549083341     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2009.06.031     Document Type: Article

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