Mitochondrial membrane potential and apoptosis of blood mononuclear cells in untreated HIV-1 infected patients

HIV Medicine

Volumn 10, Issue 8, 2009, Pages 512-519

  Sternfeld, Thomas ^a,b   Tischleder, A ^b   Schuster, M ^b   Bogner, J R ^b  

^a TECHNICAL UNIVERSITY OF MUNICH   (Germany)

^b LUDWIG MAXIMILIANS UNIVERSITY   (Germany)

Author keywords

AIDS; Apoptosis; CD4; HIV; Mitochondrial membrane potential; PBMC; Viral load

Indexed keywords

5,5',6,6' TETRAETHYLBENZIMIDAZOLOCARBOCYANINE IODIDE; DYE; LIPOCORTIN 5; UNCLASSIFIED DRUG;

ADULT; APOPTOSIS; ARTICLE; CD4 LYMPHOCYTE COUNT; CELL ACTIVITY; CELL ASSAY; CELL COUNT; CLINICAL ARTICLE; CONTROLLED STUDY; CORRELATION ANALYSIS; DISEASE DURATION; FEMALE; FLOW CYTOMETRY; HUMAN; HUMAN CELL; HUMAN IMMUNODEFICIENCY VIRUS 1 INFECTION; HUMAN IMMUNODEFICIENCY VIRUS INFECTED PATIENT; IN VIVO STUDY; MALE; MITOCHONDRIAL MEMBRANE POTENTIAL; PERIPHERAL BLOOD MONONUCLEAR CELL; PRIORITY JOURNAL; SENSITIVITY ANALYSIS; VIRUS LOAD;

ACQUIRED IMMUNODEFICIENCY SYNDROME; ADULT; APOPTOSIS; CD4 LYMPHOCYTE COUNT; CD4-POSITIVE T-LYMPHOCYTES; CROSS-SECTIONAL STUDIES; DISEASE PROGRESSION; FEMALE; FLOW CYTOMETRY; HIV INFECTIONS; HUMANS; LEUKOCYTES, MONONUCLEAR; MALE; MEMBRANE POTENTIAL, MITOCHONDRIAL; MIDDLE AGED; MITOCHONDRIAL DISEASES; PROSPECTIVE STUDIES; STAINING AND LABELING; VIRAL LOAD;

EID: 68449089436     PISSN: 14642662     EISSN: 14681293     Source Type: Journal    
DOI: 10.1111/j.1468-1293.2009.00723.x     Document Type: Article

References (43)

1. Fernandez Larrosa PN, Croci DO, Riva DA et al. Apoptosis resistance in HIV-1 persistently-infected cells is independent of active viral replication and involves modulation of the apoptotic mitochondrial pathway. Retrovirology 2008; 5: 19.
2. Terai C, Kornbluth RS, Pauza CD, Richman DD, Carson DA. Apoptosis as a mechanism of cell death in cultured T lymphoblasts acutely infected with HIV-1. J Clin Invest 1991; 87: 1710-1715.
3. Gougeon ML, Lecoeur H, Dulioust A et al. Programmed cell death in peripheral lymphocytes from HIV-infected persons: Increased susceptibility to apoptosis of CD4 and CD8T cells correlates with lymphocyte activation and with disease progression. J Immunol 1996; 156: 3509-3520.
4. Badley AD, Dockrell DH, Algeciras A et al. In vivo analysis of Fas/FasL interactions in HIV-infected patients. J Clin Invest 1998; 102: 79-87.
5. Badley AD, Parato K, Cameron DW et al. Dynamic correlation of apoptosis and immune activation during treatment of HIV infection. Cell Death Differ 1999; 6: 420-432.
6. Bohler T, Walcher J, Holzl-Wenig G et al. Early effects of antiretroviral combination therapy on activation, apoptosis and regeneration of T cells in HIV-1-infected children and adolescents. AIDS 1999; 13: 779-789.
7. Varbanov M, Espert L, Biard-Piechaczyk M. Mechanisms of CD4 T-cell depletion triggered by HIV-1 viral proteins. AIDS Rev 2006; 8: 221-236.
8. Deeks SG, Walker BD. The immune response to AIDS virus infection: Good, bad, or both? J Clin Invest 2004; 113: 808-810.
9. Grossman Z, Meier-Schellersheim M, Sousa AE, Victorino RM, Paul WE. CD4 T-cell depletion in HIV infection: Are we closer to understanding the cause? Nat Med 2002; 8: 319-323.
10. Alimonti JB, Shi L, Baijal PK, Greenberg AH. Granzyme B induces BID-mediated cytochrome c release and mitochondrial permeability transition. J Biol Chem 2001; 276: 6974-6982.
11. Korsmeyer SJ, Wei MC, Saito M et al. Pro-apoptotic cascade activates BID, which oligomerizes BAK or BAX into pores that result in the release of cytochrome c. Cell Death Differ 2000; 7: 1166-1173.
12. Cossarizza A. Tests for mitochondrial function and DNA: Potentials and pitfalls. Curr Opin Infect Dis 2003; 16: 5-10.
13. Macho A, Castedo M, Marchetti P et al. Mitochondrial dysfunctions in circulating T lymphocytes from human immunodeficiency virus-1 carriers. Blood 1995; 86: 2481-2487.
14. Cossarizza A, Baccarani-Contri M, Kalashnikova G, Franceschi C. A new method for the cytofluorimetric analysis of mitochondrial membrane potential using the J-aggregate forming lipophilic cation 5,5′,6,6′-tetrachloro-1,1′,3,3′ -tetraethylbenzimidazolcarbocyanine iodide (JC-1). Biochem Biophys Res Commun 1993; 197: 40-45.
15. Cossarizza A, Mussini C, Mongiardo N et al. Mitochondria alterations and dramatic tendency to undergo apoptosis in peripheral blood lymphocytes during acute HIV syndrome. AIDS 1997; 11: 19-26.
16. Cossarizza A, Mussini C, Borghi V et al. Apoptotic features of peripheral blood granulocytes and monocytes during primary, acute HIV infection. Exp Cell Res 1999; 247: 304-311.
17. Ferraresi R, Troiano L, Rossi D et al. Mitochondrial membrane potential and nucleosidic inhibitors of HIV reverse transcriptase: A cytometric approach. Mitochondrion 2004; 4: 271-278.
18. Lugli E, Troiano L, Ferraresi R et al. Characterization of cells with different mitochondrial membrane potential during apoptosis. Cytometry A 2005; 68: 28-35.
19. Rottenberg H, Wu S. Quantitative assay by flow cytometry of the mitochondrial membrane potential in intact cells. Biochim Biophys Acta 1998; 1404: 393-404.
20. Vayssier-Taussat M, Kreps SE, Adrie C et al. Mitochondrial membrane potential: A novel biomarker of oxidative environmental stress. Environ Health Perspect 2002; 110: 301-305.
21. Verhoeven AJ, Verhaar R, Gouwerok EG, de Korte D. The mitochondrial membrane potential in human platelets: A sensitive parameter for platelet quality. Transfusion 2005; 45: 82-89.
22. Wan B, Doumen C, Duszynski J et al. Effects of cardiac work on electrical potential gradient across mitochondrial membrane in perfused rat hearts. Am J Physiol 1993; 265: H453-H460.
23. Buttgereit F, Burmester GR, Brand MD. Bioenergetics of immune functions: fundamental and therapeutic aspects. Immunol Today 2000; 21: 192-199.
24. Green DR, Kroemer G. The pathophysiology of mitochondrial cell death. Science 2004; 305: 626-629.
25. Vermes I, Haanen C, Steffens-Nakken H, Reutelingsperger C. A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V. J Immunol Methods 1995; 184: 39-51.
26. Koopman G, Reutelingsperger CP, Kuijten GA et al. Annexin V for flow cytometric detection of phosphatidylserine expression on B cells undergoing apoptosis. Blood 1994; 84: 1415-1420.
27. Sternfeld T, Schmid M, Tischleder A et al. The influence of HIV infection and antiretroviral therapy on the mitochondrial membrane potential of peripheral mononuclear cells. Antivir Ther 2007; 12: 769-778.
28. Banas B, Kost BP, Goebel FD. Platelets, a typical source of error in real-time PCR quantification of mitochondrial DNA content in human peripheral blood cells. Eur J Med Res 2004; 9: 371-377.
29. Cossarizza A, Ceccarelli D, Masini A. Functional heterogeneity of an isolated mitochondrial population revealed by cytofluorometric analysis at the single organelle level. Exp Cell Res 1996; 222: 84-94.
30. Mathur A, Hong Y, Kemp BK, Barrientos AA, Erusalimsky JD. Evaluation of fluorescent dyes for the detection of mitochondrial membrane potential changes in cultured cardiomyocytes. Cardiovasc Res 2000; 46: 126-138.
31. Smiley ST, Reers M, Mottola-Hartshorn C et al. Intracellular heterogeneity in mitochondrial membrane potentials revealed by a J-aggregate-forming lipophilic cation JC-1. Proc Natl Acad Sci USA 1991; 88: 3671-3675.
32. Salvioli S, Ardizzoni A, Franceschi C, Cossarizza A. JC-1, but not DiOC6(3) or rhodamine 123, is a reliable fluorescent probe to assess delta psi changes in intact cells: Implications for studies on mitochondrial functionality during apoptosis. FEBS Lett 1997; 411: 77-82.
33. Tong V, Teng XW, Chang TK, Abbott FS. Valproic acid II: Effects on oxidative stress, mitochondrial membrane potential, and cytotoxicity in glutathione-depleted rat hepatocytes. Toxicol Sci 2005; 86: 436-443.
34. Risso A, Braidot E, Sordano MC et al. BMAP-28, an antibiotic peptide of innate immunity, induces cell death through opening of the mitochondrial permeability transition pore. Mol Cell Biol 2002; 22: 1926-1935.
35. Almeida A, Almeida J, Bolanos JP, Moncada S. Different responses of astrocytes and neurons to nitric oxide: The role of glycolytically generated ATP in astrocyte protection. Proc Natl Acad Sci USA 2001; 98: 15294-15299.
36. Sarkar S, Kalia V, Montelaro RC. Caspase-mediated apoptosis and cell death of rhesus macaque CD4 T-cells due to cryopreservation of peripheral blood mononuclear cells can be rescued by cytokine treatment after thawing. Cryobiology 2003; 47: 44-58.
37. Polo R, Martinez S, Madrigal P, Gonzalez-Munoz M. Factors associated with mitochondrial dysfunction in circulating peripheral blood lymphocytes from HIV-infected people. J Acquir Immune Defic Syndr 2003; 34: 32-36.
38. Gougeon ML. To kill or be killed: How HIV exhausts the immune system. Cell Death Differ 2005; 12 (Suppl 1): 845-854.
39. Lelievre JD, Mammano F, Arnoult D et al. A novel mechanism for HIV1-mediated bystander CD4 T-cell death: Neighboring dying cells drive the capacity of HIV1 to kill noncycling primary CD4 T cells. Cell Death Differ 2004; 11: 1017-1027.
40. Wasmuth JC, Klein KH, Hackbarth F et al. Prediction of imminent complications in HIV-1-infected patients by markers of lymphocyte apoptosis. J Acquir Immune Defic Syndr 2000; 23: 44-51.
41. Rothen M, Gratzl S, Hirsch HH, Moroni C. Apoptosis in HIV-infected individuals is an early marker occurring independently of high viremia. AIDS Res Hum Retroviruses 1997; 13: 771-779.
42. Alimonti JB, Ball TB, Fowke KR. Mechanisms of CD4 T lymphocyte cell death in human immunodeficiency virus infection and AIDS. J Gen Virol 2003; 84: 1649-1661.
43. Vlahakis SR, Bennett SA, Whitehead SN, Badley AD. HIV protease inhibitors modulate apoptosis signaling in vitro and in vivo. Apoptosis 2007; 12: 969-977.