Assessment of a real-time PCR test to diagnose syphilis from diverse biological samples

Sexually Transmitted Infections

Volumn 85, Issue 4, 2009, Pages 264-269

  Gayet Ageron, A ^a   Ninet, B ^a   Toutous Trellu, L ^a   Lautenschlager, S ^b   Furrer, H ^c   Piguet, V ^a   Schrenzel, J ^a   Hirschel, B ^a  

^a Geneva UniversityHospitals   (Switzerland)

^b TRIEMLI HOSPITAL   (Switzerland)

^c UNIVERSITY OF BERN   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIRETROVIRUS AGENT; AZITHROMYCIN; BENZATHINE PENICILLIN; CEFTRIAXONE; DOXYCYCLINE; PENICILLIN G;

ADULT; ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL GENE; BACTERIUM DETECTION; BLOOD SAMPLING; CEREBROSPINAL FLUID; DIAGNOSTIC ACCURACY; DRUG SUBSTITUTION; DRUG WITHDRAWAL; FEMALE; GENE SEQUENCE; HIGHLY ACTIVE ANTIRETROVIRAL THERAPY; HUMAN; HUMAN IMMUNODEFICIENCY VIRUS; HUMAN IMMUNODEFICIENCY VIRUS INFECTION; MAJOR CLINICAL STUDY; MALE; NUCLEOTIDE SEQUENCE; OUTPATIENT DEPARTMENT; PRIORITY JOURNAL; PROSPECTIVE STUDY; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SYPHILIS; SYPHILIS SEROLOGY; TREPONEMA PALLIDUM; URINALYSIS;

ADULT; ANTI-BACTERIAL AGENTS; AZITHROMYCIN; DNA, BACTERIAL; DRUG RESISTANCE, BACTERIAL; EPIDEMIOLOGIC METHODS; FEMALE; HIV SEROPOSITIVITY; HUMANS; MALE; POLYMERASE CHAIN REACTION; PREGNANCY; SEQUENCE ANALYSIS, RNA; SYPHILIS; SYPHILIS SERODIAGNOSIS; TREPONEMA PALLIDUM; UNSAFE SEX;

EID: 68249132761     PISSN: 13684973     EISSN: 14723263     Source Type: Journal    
DOI: 10.1136/sti.2008.034314     Document Type: Article

References (30)

1. Chesson HW, Dee TS, Aral SO. AIDS mortality may have contributed to the decline in syphilis rates in the United States in the 1990s. Sex Transm Dis 2003;30:419-424
2. Stolte IG, Dukers NH, Geskus RB, et al. Homosexual men change to risky sex when perceiving less threat of HIV/AIDS since availability of highly active antiretroviral therapy: a longitudinal study. AIDS 2004;18:303-309
3. Wong W, Chaw JK, Kent CK, et al. Risk factors for early syphilis among gay and bisexual men seen in an STD clinic: San Francisco, 2002-2003. Sex Transm Dis 2005;32:458-463
4. MMWR. Primary and secondary syphilis-United States, 2002. MMWR Morb Mortal Wkly Rep 2003;52:1117-1120
5. Peeling RW, Mabey D, Herring A, et al. Why do we need quality-assured diagnostic tests for sexually transmitted infections? Nat Rev Microbiol 2006;4:909-921
6. Augenbraun MH, DeHovitz JA, Feldman J, et al. Biological false-positive syphilis test results for women infected with human immunodeficiency virus. Clin Infect Dis 1994;19:1040-1044 (Pubitemid 24380015)
7. Hutchinson CM, Rompalo AM, Reichart CA, et al. Characteristics of patients with syphilis attending Baltimore STD clinics. Multiple high-risk subgroups and interactions with human immunodeficiency virus infection. Arch Intern Med 1991;151:511-516
8. Buffet M, Grange PA, Gerhardt P, et al. Diagnosing Treponema pallidum in secondary syphilis by PCR and immunohistochemistry. J Invest Dermatol 2007;127:2345-2350 (Pubitemid 47619760)
9. Michelow IC, Wendel GD Jr, Norgard MV, et al. Central nervous system infection in congenital syphilis. New Engl J Med 2002;346:1792-1798 (Pubitemid 34586915)
10. Kouznetsov AV, Weisenseel P, Trommler P, et al. Detection of the 47-kilodalton membrane immunogen gene of Treponema pallidum in various tissue sources of patients with syphilis. Diagn Microbiol Infect Dis 2005;51:143-145 (Pubitemid 40215426)
11. Kuznetsov AV, Burgdorf WH, Prinz JC. Latent syphilis confirmed by polymerase chain reaction in 2 HIV-positive patients with inconclusive serologic test results. Arch Dermatol 2005;141:1169-1170
12. Orle KA, Gates CA, Martin DH, et al. Simultaneous PCR detection of Haemophilus ducreyi, Treponema pallidum, and herpes simplex virus types 1 and 2 from genital ulcers. J Clin Microbiol 1996;34:49-54.
13. Palmer HM, Higgins SP, Herring AJ, et al. Use of PCR in the diagnosis of early syphilis in the United Kingdom. Sex Transm Infect 2003;79:479-483
14. Rodionova EN, Gushchin AE, Shipulin GA, et al. [Detection of Treponema pallidum DNA and RNA in clinical material from patients with syphilis at different stages]. Zh Mikrobiol Epidemiol Immunobiol 2003;3:43-50.
15. Wicher K, Noordhoek GT, Abbruscato F, et al. Detection of Treponema pallidum in early syphilis by DNA amplification. J Clin Microbiol 1992;30:497-500.
16. Liu H, Rodes B, Chen CY, et al. New tests for syphilis: rational design of a PCR method for detection of Treponema pallidum in clinical specimens using unique regions of the DNA polymerase I gene. J Clin Microbiol 2001;39:1941-1946 (Pubitemid 32417048)
17. Burstain JM, Grimprel E, Lukehart SA, et al. Sensitive detection of Treponema pallidum by using the polymerase chain reaction. J Clin Microbiol 1991;29:62-69
18. Yinnon AM, Coury-Doniger P, Polito R, et al. Serologic response to treatment of syphilis in patients with HIV infection. Arch Intern Med 1996;156:321-325 (Pubitemid 26058484)
19. Centers for Disease Control and Prevention. Case definitions for infectious conditions under public health surveillance. MMWR Recomm Rep 1997;46:1-55.
20. Hsu PL, Chamberlain NR, Nr C, et al. Sequence analysis of the 47-kilodalton major integral membrane immunogen of Treponema pallidum. Infect Immun 1989;57:196-203. (Pubitemid 19023201)
21. Mitchell SJ, Engelman J, Kent CK, et al. Azithromycin-resistant syphilis infection: San Francisco, California, 2000-2004. Clin Infect Dis 2006;42:337-345
22. Turner TB, Hardy PH, Newman B. Infectivity tests in syphilis. Br J Vener Dis 1969;45:183-195
23. Bruisten SM, Cairo I, Fennema H, et al. Diagnosing genital ulcer disease in a clinic for sexually transmitted diseases in Amsterdam, The Netherlands. J Clin Microbiol 2001;39:601-605 (Pubitemid 32150825)
24. Leslie DE, Azzato F, Karapanagiotidis T, et al. Development of a real-time PCR assay to detect Treponema pallidum in clinical specimens and assessment of the assay's performance by comparison with serological testing. J Clin Microbiol 2007;45:93-96 (Pubitemid 46106412)
25. Castro R, Prieto E, Aguas MJ, et al. Detection of Treponema pallidum sp pallidum DNA in latent syphilis. Int J STD AIDS 2007;18:842-845 (Pubitemid 351738866)
26. Deka RK, Machius M, Norgard MV, et al. Crystal structure of the 47-kDa lipoprotein of Treponema pallidum reveals a novel penicillin-binding protein. J Biol Chem 2002;277:41857-41864 (Pubitemid 35257497)
27. Mackay IM, Harnett G, Jeoffreys N, et al. Detection and discrimination of herpes simplex viruses, Haemophilus ducreyi, Treponema pallidum, and Calymmatobacterium (Klebsiella) granulomatis from genital ulcers. Clin Infect Dis 2006;42:1431-1438
28. Salazar JC, Rathi A, Michael NL, et al. Assessment of the kinetics of Treponema pallidum dissemination into blood and tissues in experimental syphilis by real-time quantitative PCR. Infect Immun 2007;75:2954-2958 (Pubitemid 46870177)
29. Engelkens HJ, ten Kate FJ, Judanarso J, et al. The localisation of treponemes and characterisation of the inflammatory infiltrate in skin biopsies from patients with primary or secondary syphilis, or early infectious yaws. Genitourin Med 1993;69:102-107
30. Engelkens HJ, ten Kate FJ, Vuzevski VD, et al. Primary and secondary syphilis: a histopathological study. Int J STD AIDS 1991;2:280-284