New tests for syphilis: Rational design of a PCR method for detection of Treponema pallidum in clinical specimens using unique regions of the DNA polymerase I gene

Journal of Clinical Microbiology

Volumn 39, Issue 5, 2001, Pages 1941-1946

  Liu, H ^a   Rodes, B ^a   Chen, C Y ^a   Steiner, B ^a  

^a CENTERS FOR DISEASE CONTROL AND PREVENTION   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA DIRECTED DNA POLYMERASE BETA;

ARTICLE; BACTERIUM DETECTION; DIAGNOSTIC ACCURACY; DIAGNOSTIC VALUE; GENE INSERTION; GENITAL ULCER; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY; SPECIES DIFFERENCE; SYPHILIS; TREPONEMA PALLIDUM;

AMINO ACID SEQUENCE; DNA POLYMERASE I; DNA PRIMERS; GENES, BACTERIAL; HUMANS; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SEQUENCE ANALYSIS, DNA; SYPHILIS; TREPONEMA PALLIDUM;

EID: 0035002989     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.39.5.1941-1946.2001     Document Type: Article

References (22)

1. Identification and characterization of immunoglobulin G in blood as a major inhibitor of diagnostic PCR
2. Sensitive detection of Treponema pallidum by using the polymerase chain reaction
3. Detection of Treponema pallidum by a sensitive reverse transcriptase PCR
4. Multiple alleles of Treponema pallidum repeat gene D in Treponema pallidum isolates
5. Human immunodeficiency virus infection and genital ulcer disease in South Africa. The herpetic connection
6. Use of polymerase chain reaction and rabbit infectivity testing to detect Treponema pallidum in amniofic fluid
7. Use of polymerase chain reaction to detect DNA sequences specific to pathogenic treponemes in cerebrospinal fluid
8. Detection of treponemal DNA in the CSF of patients with syphilis and HIV infection using polymerase chain reaction
9. Inhibition of human immunodeficiency virus gene amplification by heparin
10. Brief report: Cerebral syphilitic gumma confirmed by the polymerase chain reaction in a man with human immunodeficiency virus infection
11. Comparison of molecular and microscopic techniques for detection of Treponema pallidum in genital ulcers
12. Laboratory diagnosis and interpretation of tests for syphilis
13. Treponema and other host-associated spirochetes
14. Detection by polymerase chain reaction of Treponema pallidum DNA in cerebrospinal fluid from neurosyphilis patients before and after antibiotic treatment
15. Simultaneous PCR detection of Haemophilus ducreyi, Treponema pallidum, and herpes simplex virus types 1 and 2 from genital ulcers
16. Molecular subtyping of Treponema pallidum subspecies pallidum
17. Cloning and characterization of a gene (polA) coding for an unusual DNA polymerase 1 from the obligate parasite Treponema pallidum
18. Evaluation of molecular methodologies and rabbit infectivity testing for the diagnosis of congenital syphilis and neonatal central nervous system invasion by Treponema pallidum
19. Strategies for syphilis prevention in the 1990s
20. Infectivity tests in syphilis
21. The 47-kDa major lipoprotein immunogen of Treponema pallidum is a penicillin-binding protein with carboxypeptidase activity
22. Detection of Treponema pallidum in early syphilis by DNA amplification