Detection of Treponema pallidum sp pallidum DNA in latent syphilis

International Journal of STD and AIDS

Volumn 18, Issue 12, 2007, Pages 842-845

  Castro, Rita ^a   Prieto, E ^a   Aguas M J ^b   Manata, M J ^b   Botas, J ^b   Santo, I ^c   Azevedo, J ^c   Pereira, F L H ^a  

^a INSTITUTO DE HIGIENE E MEDICINA TROPICAL   (Portugal)

^b HOSPITAL GARCIA DE ORTA   (Portugal)

^c Ministerio da Saude Portuguese   (Portugal)

Author keywords

Latent syphilis; PCR; Syphilis; T. pallidum

Indexed keywords

BACTERIAL DNA;

ARTICLE; BACTERIUM DETECTION; BLOOD SAMPLING; CONTROLLED STUDY; EAR; HUMAN; MAJOR CLINICAL STUDY; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SEROLOGY; SYPHILIS; TREPONEMA PALLIDUM; BLOOD; CLASSIFICATION; EVALUATION; GENETICS; ISOLATION AND PURIFICATION; LABORATORY DIAGNOSIS; METHODOLOGY; MICROBIOLOGY; PLASMA; SERUM;

BLOOD; DNA, BACTERIAL; EAR; HUMANS; PLASMA; POLYMERASE CHAIN REACTION; SERUM; SPECIMEN HANDLING; SYPHILIS, LATENT; TREPONEMA PALLIDUM;

EID: 39549120962     PISSN: 09564624     EISSN: None     Source Type: Journal    
DOI: 10.1258/095646207782716901     Document Type: Article

References (19)

1. Centers for Disease Control and Prevention (CDC). Trends in primary and secondary syphilis and HIV infections in men who have sex with men - San Francisco and Los Angeles, California, 1998-2002. MMWR 2004;53:575-8
2. Fenton KA. A multilevel approach to understanding the resurgence and evolution of infectious syphilis in Western Europe. Euro Surveill 2004;9:3-4
3. Fleming DT, Wasserheit JN. From epidemiological synergy to public health policy and practice: the contribution of other sexually transmitted diseases to sexual transmission of HIV infection. Sex Transm Infect 1999;75:3-17
4. Larsen SA, Johnson RE. Diagnostic tests. In: Larsen S, Pope V, Johnson RE, et al., eds. A Manual of Tests for Syphilis. 9th edn. Washington, DC: American Public Health Association, 1998;1-52
5. Orle KA, Gates CA, Martin DH, Body BA, Weiss JB. Simultaneous PCR detection of Haemophilus ducreyi, Treponema pallidum, and herpes simplex virus types 1 and 2 from genital ulcers. J Clin Microbiol 1996;34:49-54
6. Liu H, Rodes B, Chen CY, Steiner B. New tests for syphilis: rational design of a PCR method for detection of Treponema pallidum in clinical specimens using unique regions of the DNA polymerase I gene. J Clin Microbiol 2001;39:1941-6
7. Palmer HM, Higgins SP, Herring AJ, Kingston MA. Use of PCR in the diagnosis of early syphilis in the United Kingdom. Sex Transm Infect 2003;79:479-83
8. Marfin AA, Liu H, Sutton MY, Steiner B, Pillay A, Markowitz LE. Amplification of the DNA polymerase I gene of Treponema pallidum from whole blood of persons with syphilis. Diagn Microbiol Infect Dis 2001;40:163-6
9. Sutton MY, Liu H, Steiner B, et al. Molecular subtyping of Treponema pallidum in Arizona County with increasing syphilis morbidity: use of specimens from ulcers and blood. J Infect Dis 2001;183: 1601-6
10. Marra CM, Gary DW, Kuypers J, Jacobson MA. Diagnosis of neurosyphilis in patients infected with human immuno-deficiency virus type 1. J Infect Dis 1996;174:219-21
11. Chung KY, Lee MG, Lee JB. Detection of Treponema pallidum by polymerase chain reaction in the cerebrospinal fluid of syphilis patients. Yonsei Med J 1994;35:190-7
12. Workowski KA, Berman SM, Centers for Disease Control and Prevention. Sexually transmitted diseases treatment guidelines, 2006. MMWR Recomm Rep 2006;55(RR-11):1-94; erratum in: MMWR Recomm Rep 2006;55:997
13. Goh BT, van Voorst Vade PC, European Branch of the International Union against Sexually Transmitted Infection and the European Office of the World. European guideline for the management of syphilis. Int J STD AIDS 2001;12:14-26
14. Larsen SA, Steiner BM, Rudolph AH. Laboratory diagnosis and interpretation of tests for syphilis. Clin Microbiol Rev 1995;8:1-21
15. Grimprel E, Sanchez PJ, Wendel GD, et al. Use of polymerase chain reaction and rabbit infectivity testing to detect Treponema pallidum in amniotic fluid, fetal and neonatal sera, and cerebrospinal fluid. J Clin Microbiol 1991;29:1711-18
16. Wicher K, Abbruscato F, Wicher V, Collins DN, Auger I, Horowitz HW. Identification of persistent infection in experimental syphilis by PCR. Infect Immun 1998;66:2509-13
17. Pietravalle M, Pimpinelli F, Maini A, et al. Diagnostic relevance of polymerase chain reaction technology for T. pallidum in subjects with syphilis in different phases of infection. New Microbiol 1999;22:99-104
18. Tramont EC. Spirochetes. Treponema pallidum (syphilis). In: Mandell GL, Bennett JE, Dalin R, eds. Principles and Practice of Infectious Diseases. 4th edn. New York: Churchill Livingstone, 1995;2117-33
19. Orton SH, Liu H, Dodd RY, Williams AE. Prevalence of circulating Treponema pallidum DNA and RNA in blood donors with confirmed-positive syphilis tests. Transfusion 2002;42:94-9