Simultaneous PCR detection of Haemophilus ducreyi, treponema pallidum, and herpes simplex virus types 1 and 2 from genital ulcers

Journal of Clinical Microbiology

Volumn 34, Issue 1, 1996, Pages 49-54

  Orle, Karina A ^a   Gates, Carol A ^a   Martin, David H ^b   Body, Barbara A ^c   Weiss, Judith B ^a  

^a ROCHE MOLECULAR SYSTEMS   (United States)

^b LOUISIANA STATE UNIV MEDICAL CENTER   (United States)

^c LABORATORY CORPORATION OF AMERICA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

HAEMOPHILUS DUCREYI; HERPES; SIMPLEXVIRUS; TREPONEMA PALLIDUM;

ARTICLE; BACTERIUM CULTURE; COLORIMETRY; DIAGNOSTIC TEST; GENE AMPLIFICATION; GENE SEQUENCE; GENITAL ULCER; HAEMOPHILUS DUCREYI; HERPES SIMPLEX VIRUS 1; HUMAN; HUMAN TISSUE; MAJOR CLINICAL STUDY; MALE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; TREPONEMA PALLIDUM;

BACTERIOLOGICAL TECHNIQUES; BASE SEQUENCE; CHANCROID; COLORIMETRY; COMPARATIVE STUDY; DNA PRIMERS; DNA PROBES; DNA, BACTERIAL; DNA, VIRAL; GENITAL DISEASES, MALE; HAEMOPHILUS DUCREYI; HERPES GENITALIS; HERPESVIRUS 1, HUMAN; HERPESVIRUS 2, HUMAN; HUMAN; MALE; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SYPHILIS; TREPONEMA PALLIDUM; ULCER; VIROLOGY;

EID: 0029656079     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/jcm.34.1.49-54.1996     Document Type: Article

References (37)

1. Boulos, R., A. J. Ruff, A. Nahmias, E. Holt, L. Harrison, L. Madger, S. Z. Wiktor, T. C. Quinn, H. Margolis, N. A. Halsey, and the Johns Hopkins University/Center pour le Developpement et la Santé HIV Study Group. 1992. Herpes simplex virus type 2 infection, syphilis, and hepatitis B virus infection in Haitian women with human immunodeficiency virus type 1 and human T lymphotropic virus type I infections. J. Infect. Dis. 166:418-420.
2. Burstain, J. M., E. Gimprel, S. A. Lukehart, M. V. Norgard, and J. D. Radolf. 1991. Sensitive detection of Treponema pallidum by using the polymerase chain reaction. J. Clin. Microbiol. 29:62-69.
3. Bzik, D. J., C. Debroy, B. A. Fox, N. E. Pederson, and S. Person. 1980. The nucleotide sequence of the gB glycoprotein of HSV-2 and comparison with the corresponding gene of HSV-1. Virology 155:322-333.
4. Chui, L., W. Albritton, B. Paster, I. Maclean, and R. Marusyk. 1993. Development of the polymerase chain reaction assay for the detection of Haemophilus ducreyi. J. Clin. Microbiol. 31:659-664.
5. Cohen, B. A., A. H. Rowley, and C. M. Long. 1994 Herpes simplex type 2 in a patient with Mollaret's meningitis: demonstration by polymerase chain reaction. Ann. Neurol. 35:112-116.
6. Cone, R. W., A. C. Hobson, Z. Brown, R. A. Ashley, S. Berry, C. Winter, and L. Corey. 1994. Frequent detection of genital herpes simplex virus by polymerase chain reaction among pregnant women JAMA 272:792-796.
7. Cone, R. W., A. C. Hobson, J. Palmer, M. Remington, and L. Corey. 1991. Extended duration of herpes simplex virus DNA in genital lesions detected by the polymerase chain reaction. J. Infect. Dis. 164:757-760.
8. Dallas, W. S., P. H. Ray, J. Leong, C. D. Benedict, L. V. Stamm, and P. J. Bassford, Jr. 1987. Identification and purification of a recombinant Treponema pallidum basic membrane protein antigen expressed in Escherichia coli. Infect. Immun 55:1106-1115.
9. Dewhirst, F. E., B. J. Paster, I. Olsen, and G. J. Fraser. 1992. Phylogeny of 54 representative strains of species in the family Pasteurellaceae as determined by comparison of 16S rRNA sequences. J. Bacteriol 174:2002-2013.
10. Greisen, K., M. Loeffelholz, A. Purohit, and D. Leong. 1994 PCR primers and probes for the 16S rRNA gene of most species of pathogenic bacteria, including bacteria found in cerebrospinal fluid. J. Clin. Microbiol. 32:335-351.
11. Grimprel, E., P. J. Sanchez, G. D. Wendel, J. M. Burstain, G. H. McCracken, Jr., J. D. Radolf, and M. V. Norgard. 1991. Use of polymerase chain reaction and rabbit infectivity testing to detect Treponema pallidum in amniotic fluid, fetal and neonatal sera, and cerebrospinal fluid. J. Clin Microbiol 29:1711-1718
12. Hardy, D. A., A. M. Arvin, L. L. Yasukawa, R. N. Bronzan, D. M. Lewinsohn, P. A. Hensleigh, and C. G. Prober. 1990. Use of polymerase chain reaction for successful identification of asymptomatic genital infection with herpes simplex virus in pregnant women at delivery. J. Infect. Dis. 162:1031-1035.
13. Hay, P. E., J. R. Clarke, R. A. Strugnell, D. Taylor-Robinson, and D. Goldmeier. 1990. Use of the polymerase chain reaction to detect DNA sequences specific to pathogenic treponemes in cerebrospinal fluid. FEMS Microbiol Lett. 68:233-238.
14. Jessamine, P. G., F. A. Plummer, J. O. Ndinya-Achola, M. A. Wainberg, I. Wamola, L. J. D'Costa, D. W. Cameron, J. N. Simonsen, P. Plourde, and A. R. Ronald. 1990. Human immunodeficiency virus, genital ulcers and the male foreskin: synergism in HIV-1 transmission. Scand. J. Infect. Dis. Suppl. 69:181-186.
15. Jethwa, H. S., J. L. Schmitz, G. Dallabetta, F. Behets, I. Hoffman, H. Hamilton, G. Lule, M. Cohen, and J. D. Folds. 1995. Comparison of molecular and microscopic techniques for detection of Treponema pallidum in genital ulcers. J. Clin. Microbiol. 33:180-183.
16. Johnson, S. R., D. H. Martin, C. Cammarata, and S. A. Morse. 1995. Alterations in sample preparation increase sensitivity of PCR assay for diagnosis of chancroid. J. Clin. Microbiol. 33:1036-1038.
17. Kimura, H., M. Shibata, K. Kuzushima, Y. Nishiyama, and T. Morishima. 1990. Detection and direct typing of herpes simplex virus by polymerase chain reaction. Med Microbiol. Immunol. 179:177-184.
18. Koutsky, L. A., C. E. Stevens, K. K. Holmes, R. L. Ashly, N. B. Kiviat, C. W. Critchlow, and L. Corey. 1992. Underdiagnosis of genital herpes by current clinical and viral-isolation procedures. N. Engl. J Med. 326:1533-1539.
19. Kreis; J. K. R. Coombs, F. Plummer, K. K. Holmes, B. Nikora, W. Cameron, E. Ngugi, J. O. Ndinya-Achola, and L. Corey. 1989 Isolation of human immunodeficiency virus from genital ulcers in Nairobi prostitutes. J Infect. Dis. 160:380-384.
20. Lakeman, F. D., R. J. Whitley, and the National Institute of Allergy and Infectious Diseases Collaborative Antiviral Study Group. 1995. Diagnosis of herpes simplex encephalitis: application of polymerase chain reaction to cerebrospinal fluid from brain-biopsied patients and correlation with disease. J Infect Dis. 171:857-863
21. Larsen, S. A., B. M. Steiner, and A. H. Rudolf. 1995. Laboratory diagnosis and interpretation of tests for syphilis. Clin. Microbiol. Rev. 8:1-21.
22. Loeffelholz, M. J., C. A. Lewinski, S. R. Silver, A. P. Purohit, S. A. Herman, D. A. Buonagurio, and E. A. Dragon. 1992. Detection of Chlamydia trachomatis in endocervical specimens by polymerase chain reaction. J. Clin. Microbiol. 30:2847-2851.
23. Maniatis, T., E. F. Fritsch, and J. Sambrook. 1982 Molecular cloning: a laboratory manual, p. 89-96. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
24. Morse, S. A. 1989. Chancroid and Haemophilus ducreyi. Clin Microbiol. Rev. 2:137-157
25. Noordhoek, G. T., E. C. Wolters, M. E. J. de Jonge, and J. D. A. van Embden. 1991. Detection by polymerase chain reaction of Treponema pallidum DNA in cerebrospinal fluid from neurosyphilis patients before and after antibiotic treatment. J. Clin. Microbiol. 29:1976-1984.
26. Orle, K. A., D. H. Martin, C. A. Gates, and J. B. Weiss. 1995 A sensitive and rapid multiplex PCR assay detects Haemophilus ducreyi, Treponema pallidum, and herpes simplex viruses from genital ulcers, abstr C-247, p. 43. In Abstracts, of the 95th General Meeting of the American Society for Microbiology, 1995. American Society for Microbiology, Washington, D.C
27. Parsons, L. M., A. L. Waring, and M. Shayegani. 1992. Molecular analysis of the Haemophilus ducreyi groE heat shock operon. Infect Immun. 60:4111-4118.
28. Romanowski, B., E. Forsey, E. Prasad, S. Lukehart, M. Tam, and E. W. Hook. 1986. Detection of Treponema pallidum by a fluorescent antibody test. Sex. Transm. Dis. 14:156-159.
29. Schulte, J. M., F. A. Martich, and G. P. Schmid. 1992. Chancroid in the United States, 1981-1990: evidence for underreporting of cases. Morbid. Mortal. Weekly Rep. 41:57-61.
30. Sturm, A. W., G. J. Stolting, R. H. Cormane, and H. C. Zanen. 1987. Clinical and microbiological evaluation of 46 episodes of genital ulceration. Genitourin. Med. 63:98-101.
31. Telzak, E. E., M. A. Chiasson, P. J. Bevier, R. L. Stoneburner, K. G. Castro, and H. W. Jaffe. 1993, HIV-1 seroconversion in patients with and without genital ulcer disease A prospective study. Ann. Intern. Med 199:1181-1186.
32. Tsurumi, T., K. Maeno, and Y. Nishiyama. 1987. Nucleotide sequence of the DNA polymerase gene of herpes simplex virus type 2 and comparison with the type I counterpart. Gene 52:129-137
33. Tung, C. H., M. J. Rudolf, and S. Stein. 1991. Preparation of oligonucleotide-peptide conjugates Bioconjugate Chem 2:464-465.
34. Wasserheit, J. N. 1992. Epidemiological synergy interrelationships between human immunodeficiency virus infection and other sexually transmitted diseases. Sex. Transm. Dis. 19:61-77.
35. Weigel, L. M., M. E. Brandt, and M. V. Norgard. 1992. Analysis of the N-termmal region of the 47-kilodalton integral membrane lipoprotein of Treponema pallidum. Infect. Immun. 60:1568-1576
36. White, T. J., T. Burns, S. Lee, and J. Taylor. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenentics, p. 315-322. In M. A. Innis, D. H. Gelfand, J J. Sninsky, and T J. White (ed.), PCR protocols: a guide to methods and applications. Academic Press, San Diego, Calif.
37. Wicher, K., G. T. Noordhoek, F. Abbruscato, and V. Wicher. 1992 Detection of Treponema pallidum in early syphilis by DNA amplification J. Clin. Microbiol. 30:497-500