Coexistence of both oleosin isoforms on the surface of seed oil bodies and their individual stabilization to the organelles

Journal of Biochemistry

Volumn 123, Issue 2, 1998, Pages 318-323

  Tzen, Jason T C ^a   Chuang, Rachel L C ^a   Chen, Jeff C F ^a   Wu, Lawrence S H ^a  

^a NATIONAL CHUNG HSING UNIVERSITY   (Taiwan)

Author keywords

Immunofluorescence; Isoform; Oil body; Oleosin; Organelle stability

Indexed keywords

FLUORESCEIN ISOTHIOCYANATE; TRIACYLGLYCEROL; VEGETABLE OIL;

ARTICLE; CELL ORGANELLE; IMMUNOFLUORESCENCE; MOLECULAR WEIGHT; PLANT SEED;

EID: 0031936579     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jbchem.a021939     Document Type: Article

References (19)

1. Yatsu, L.Y. and Jacks, T.J. (1972) Spherosome membranes. Half unit-membranes. Plant Physiol. 49, 937-943
2. Stymne, S. and Stobart, A.K. (1987) Triacylglycerol biosynthesis in The Biochemistry of Plants (Stumpf, P.K. and Conn, E.E., eds.) Vol. 10, pp. 175-214, Academic Press, New York
3. Huang, A.H.C. (1992) Oil bodies and oleosins in seeds. Annu. Rev. Plant Physiol. 43, 177-200
4. Slack, C.R., Bertaud, W.S., Shaw, B.P., Holland, R., Browse, J., and Wright, H. (1980) Some studies on the composition and surface properties of oil bodies from the seed cotyledons of safflower and linseed. Biochem. J. 190, 551-561
5. Tzen, J.T.C., Lie, G.C., and Huang, A.H.C. (1992) Characterization of the charged components and their topology on the surface of plant seed oil bodies. J. Biol. Chem. 267, 15626-15634
6. Tzen, J.T.C. and Huang, A.H.C. (1992) Surface structure and properties of plant seed oil bodies. J. Cell Biol. 117, 327-335
7. Tzen, J.T.C., Cao, Y.Z., Laurent, P., Ratnayake, C., and Huang, A.H.C. (1993) Lipids, proteins, and structure of seed oil bodies from diverse species. Plant Physiol. 101, 267-276
8. Murphy, D.J., Keen, J.N., O'Sullivan, J.N., Au, D.M.Y., Edwards, E.W., Jackson, P.J., Cummins, I., Gibbons, T., Shaw, C.H., and Ryan, A.J. (1991) A class of amphipathic proteins associated with lipid storage bodies plants. Possible similarities with animal serum apolipoproteins. Biochim. Biophys. Acta 1088, 86-94
9. Li, M., Smith, L.J., Clark, D.C., Wilson, R., and Murphy, D.J. (1992) Secondary structures of a new class of lipid body proteins from oilseeds. J. Biol. Chem. 267, 8245-8253
10. Qu, R., Wang, S.M., Lin, Y.H., Vance, V.B., and Huang, A.H.C. (1986) Characteristics and biosynthesis of membrane proteins of lipid bodies in the scutella of maize (Zea mays L.). Biochem. J. 234, 57-65
11. Murphy, D.J. and Au, D.M.Y. (1989) A new class of highly abundant apolipoproteins involved in lipid storage in oilseeds. Biochem. Soc. Trans. 117, 682-683
12. Tzen, J.T.C., Lai, Y.K., Chan, K.L., and Huang, A.H.C. (1990) Oleosin isoforms of high and low molecular weights are present in the oil bodies of diverse seed species. Plant Physiol. 94, 1282-1289
13. Tzen, J.T.C., Peng, C.C., Cheng, D.J., Chen, E.C.F., and Chiu, J.M.H. (1997) A new method for seed oil body purification and examination of oil body integrity following germination. J. Biochem. 121, 762-768
14. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685
15. Chuang, R.L.C., Chen, J.C.F., Chu, J., and Tzen, J.T.C. (1996) Characterization of seed oil bodies and their surface oleosin isoforms from rice embryos. J. Biochem. 120, 74-81
16. Wilson, M.B. and Nakane, P.K. (1978) Recent development in the periodate method of conjugating horseradish peroxidase (HRPO) to antibodies in Immunofluorescence and Related Staining Techniques (Knapp, W., Holubar, K., and Wick, G., eds.) pp. 215-224, Elsevier/North Holland Biomedical Press, Amsterdam
17. Chen, P.W., Chai, Y.J., Wang, L.D., Tzen, J.T.C., Tseng, M.J., and Chen, L.J. (1996) Two embryo-specific cDNAs encoding two oleosin isoforms on surface of oil bodies from rice. Plant Physiol. 110, 714
18. Lee, K. and Huang, A.H.C. (1994) Genes encoding oleosins in maize kernel of inbreds Mo17 and B73. Plant Mol. Biol. 26, 1981-1987
19. Lee, K., Bih, F.Y., Learn, G.M., Ting, J.T.L., Sellers, C., and Huang, A.H.C. (1994) Oleosins in the gametophytes of Pinus and Brassica and their phylogenetic relationship with those in sporophytes of various species. Planta 193, 461-469