MGMT methylation in diffuse large B-cell lymphoma: Validation of quantitative methylation-specific PCR and comparison with MGMT protein expression

Journal of Clinical Pathology

Volumn 62, Issue 8, 2009, Pages 715-723

  Uccella, S ^a   Cerutti, R ^a   Placidi, C ^a   Marchet, S ^a   Carnevali, I ^a   Bernasconi, B ^a   Proserpio, I ^b   Pinotti, G ^b   Tibiletti, M G ^b   Furlan, D ^a   Capella, C ^a  

^a UNIVERSITY OF INSUBRIA   (Italy)

^b OSPEDALE DI CIRCOLO   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

METHYLATED DNA PROTEIN CYSTEINE METHYLTRANSFERASE;

ADULT; AGED; ARTICLE; CANCER SURVIVAL; CONTROLLED STUDY; DNA METHYLATION; FEMALE; HUMAN; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; IMMUNOREACTIVITY; LARGE CELL LYMPHOMA; MAJOR CLINICAL STUDY; MALE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN EXPRESSION;

ADULT; AGED; AGED, 80 AND OVER; CHROMOSOMES, HUMAN, PAIR 10; DNA METHYLATION; DNA MODIFICATION METHYLASES; DNA REPAIR ENZYMES; DNA, NEOPLASM; FEMALE; HUMANS; IN SITU HYBRIDIZATION, FLUORESCENCE; LYMPHOMA, LARGE B-CELL, DIFFUSE; MALE; MIDDLE AGED; NEOPLASM PROTEINS; NEOPLASM STAGING; POLYMERASE CHAIN REACTION; SURVIVAL ANALYSIS; TUMOR MARKERS, BIOLOGICAL; TUMOR SUPPRESSOR PROTEINS;

EID: 68149100834     PISSN: 00219746     EISSN: 14724146     Source Type: Journal    
DOI: 10.1136/jcp.2009.064741     Document Type: Article

References (31)

1. Margison GP, Santibanez-Koref MF. O6-alkylguanine-DNA alkyltransferase: role in carcinogenesis and chemotherapy. Bioessays 2002;24:255-266
2. Gerson SL. MGMT: its role in cancer aetiology and cancer therapeutics. Nat Rev Cancer 2004;4:296-307. (Pubitemid 38525285)
3. Margison GP, Povey AC, Kaina B, et al. Variability and regulation of O6-alkylguanine- DNA alkyltransferase. Carcinogenesis 2003;24:625-635 (Pubitemid 36592396)
4. Becker K, Gregel CM, Kaina B. The DNA repair protein O6-methylguanine-DNA methyltransferase protects against skin tumor formation induced by antineoplastic chloroethylnitrosourea. Cancer Res 1997;57:3335-3338 (Pubitemid 27355469)
5. Sakumi K, Shiraishi A, Shimizu S, et al. Methylnitrosourea-induced tumorigenesis in MGMT gene knockout mice. Cancer Res 1997;57:2415-2418 (Pubitemid 27255900)
6. Danam RP, Qian XC, Howell SR, et al. Methylation of selected CpGs in the human O6-methylguanine-DNA methyltransferase promoter region as a marker of gene silencing. Mol Carcinog 1999;24:85-89
7. Esteller M, Hamilton SR, Burger PC, et al. Inactivation of the DNA repair gene O6- methylguanine-DNA methyltransferase by promoter hypermethylation is a common event in primary human neoplasia. Cancer Res 1999;59:793-797 (Pubitemid 29086728)
8. Hegi ME, Diserens AC, Gorlia T, et al. MGMT gene silencing and benefit from temozolomide in glioblastoma. N Engl J Med 2005;352:997-1003. (Pubitemid 40349502)
9. Everhard S, Kaloshi G, Crinière E, et al. MGMT methylation: a marker of response to temozolomide in low-grade gliomas. Ann Neurol 2006;60:740-743
10. Stein H, Warnke RA, Chan WC, et al. Diffuse large B-cell lymphoma, not otherwise specified. In: Swerdlow SH, Campo E, Harris NL, et al, eds. WHO classification of tumors of haematopoietic and lymphoid tissues. Lyon: IARC Press, 2007:233-237
11. Esteller M, Gaidano G, Goodman SN, et al. Hypermethylation of the DNA repair gene O(6)-methylguanine DNA methyltransferase and survival of patients with diffuse large B-cell lymphoma. J Natl Cancer Inst 2002;94:26-32.
12. Rossi D, Capello D, Gloghini A, et al. Aberrant promoter methylation of multiple genes throughout the clinico-pathologic spectrum of B-cell neoplasia. Haematologica 2004;89:154-164 (Pubitemid 38796489)
13. Pike BL, Greiner TC, Wang X, et al. DNA methylation profiles in diffuse large B-cell lymphoma and their relationship to gene expression status. Leukemia 2008;22:1035-1043 (Pubitemid 351689886)
14. Herman JG, Graff JR, Myöhänen S, et al. Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands. Proc Natl Acad Sci USA 1996;93:9821-9826 (Pubitemid 26296693)
15. Derks S, Lentjes MH, Hellebrekers DM, et al. Methylation-specific PCR unraveled. Cell Oncol 2004;26:291-299
16. Uccella S, Placidi C, Marchet S, et al. BCL-6 protein expression, and not the germinal centre immunophenotype, predicts favourable prognosis in a series of primary nodal diffuse large B-cell lymphomas: a single centre experience. Leukemia Lymphoma 2008;49:1321-1328 (Pubitemid 352015795)
17. Hans CP, Weisenburger DD, Greiner TC, et al. Confirmation of the molecular classification of diffuse large B-cell lymphoma by immunohistochemistry using a tissue microarray. Blood 2004;103:275-282
18. Brent TP, von Wronski M, Pegram CN, et al. Immunoaffinity purification of human O6-alkylguanine-DNA alkyltransferase using newly developed monoclonal antibodies. Cancer Res 1990;50:58-61.
19. Eads CA, Danenberg KD, Kawakami K, et al. MethyLight: a high-throughput assay to measure DNA methylation. Nucleic Acids Res 2000;28:e32.
20. Ogino S, Brahmandam M, Kawasaki T, et al. Epigenetic profiling of synchronous colorectal neoplasias by quantitative DNA methylation analysis. Mod Pathol 2006;19:1083-1090 (Pubitemid 44106723)
21. Furlan D, Carnevali I, Marcomini B, et al. The high frequency of de novo promoter methylation in synchronous primary endometrial and ovarian carcinomas. Clin Cancer Res 2006;12:3329-3336 (Pubitemid 43910876)
22. Paternoster SF, Brockman SR, McClure RF, et al. A new method to extract nuclei from paraffin-embedded tissue to study lymphomas using interphase fluorescence in situ hybridization. Am J Pathol 2002;160:1967-1972
23. Cheson BD, Pfistner BJuweid ME, et al. The international harmonization project on lymphoma. Revised response criteria for malignant lymphoma. J Clin Oncol 2007;25:579-586
24. Vlassenbroeck I, Califice S, Diserens AC, et al. Validation of real-time methylationspecific PCR to determine O6-methylguanine-DNA methyltransferase gene promoter methylation in glioma. J Mol Diagn 2008;10:332-337 (Pubitemid 352019119)
25. Ohno T, Hiraga J, Ohashi H, et al. Loss of O6-methylguanine-DNA methyltransferase protein expression is a favorable prognostic marker in diffuse large B-cell lymphoma. Int J Hematol 2006;83:341-347 (Pubitemid 43807580)
26. Costello JF, Futscher BW, Tano K, et al. Graded methylation in the promoter and body of the O6-methylguanine DNA methyltransferase (MGMT) gene correlates with MGMT expression in human glioma cells. J Biol Chem 1994;269:17228-17237 (Pubitemid 24204047)
27. Cameron EE, Baylin SB, Herman JG. p15(INK4B) CpG island methylation in primary acute leukemia is heterogeneous and suggests density as a critical factor for transcriptional silencing. Blood 1999;94:2445-2451
28. Nakagawachi T, Soejima H, Urano T, et al. Silencing effect of CpG island hypermethylation and histone modifications on O6-methylguanine-DNA methyltransferase (MGMT) gene expression in human cancer. Oncogene 2003;22:8835-8844 (Pubitemid 38021499)
29. Zhao W, Soejima H, Higashimoto K, et al. The essential role of histone H3 Lys9 di-methylation and MeCP2 binding in MGMT silencing with poor DNA methylation of the promoter CpG island. J Biochem 2005;137:431-440 (Pubitemid 40628452)
30. Danam RP, Howell SR, Brent TP, et al. Epigenetic regulation of O6-methylguanine- DNA methyltransferase gene expression by histone acetylation and methyl-CpG binding proteins. Mol Cancer Ther 2005;4:61-69
31. Al-Kuraya K, Narayanappa R, Siraj AK, et al. High frequency and strong prognostic relevance of O6-methylguanine DNA methyltransferase silencing in diffuse large B-cell lymphomas from the Middle East. Human Pathology 2006;37:742-748 (Pubitemid 43956546)